Evolution and some functions of the NprR–NprRB quorum-sensing system in the Bacillus cereus group

Rocha, Jorge; Flores, Victor Martín Quintana; Cabrera, Rosina; Soto-Guzman, Adriana; Granados, G.; Juaristi, Eusebio; Guarneros, Gabriel; Torre, Mayra de la

doi:10.1007/s00253-011-3775-4

Cited by 28 publications

(28 citation statements)

References 37 publications

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“…We also observed sequence duplications (either intragenic or full-gene) events in rap-phr loci of other Bacilli (S11A–S11C Fig). A similar analysis of the related NprR-NprX quorum-sensing family [58,59] showed duplications in some nprX genes as well (S11D Fig, Methods). These results indicate that autoinducer duplications are abundant and that putative autoinducer sequences diverge after duplication.…”

Section: Resultsmentioning

confidence: 73%

Transient Duplication-Dependent Divergence and Horizontal Transfer Underlie the Evolutionary Dynamics of Bacterial Cell–Cell Signaling

et al. 2016

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Evolutionary expansion of signaling pathway families often underlies the evolution of regulatory complexity. Expansion requires the acquisition of a novel homologous pathway and the diversification of pathway specificity. Acquisition can occur either vertically, by duplication, or through horizontal transfer, while divergence of specificity is thought to occur through a promiscuous protein intermediate. The way by which these mechanisms shape the evolution of rapidly diverging signaling families is unclear. Here, we examine this question using the highly diversified Rap-Phr cell–cell signaling system, which has undergone massive expansion in the genus Bacillus. To this end, genomic sequence analysis of >300 Bacilli genomes was combined with experimental analysis of the interaction of Rap receptors with Phr autoinducers and downstream targets. Rap-Phr expansion is shown to have occurred independently in multiple Bacillus lineages, with >80 different putative rap-phr alleles evolving in the Bacillius subtilis group alone. The specificity of many rap-phr alleles and the rapid gain and loss of Rap targets are experimentally demonstrated. Strikingly, both horizontal and vertical processes were shown to participate in this expansion, each with a distinct role. Horizontal gene transfer governs the acquisition of already diverged rap-phr alleles, while intralocus duplication and divergence of the phr gene create the promiscuous intermediate required for the divergence of Rap-Phr specificity. Our results suggest a novel role for transient gene duplication and divergence during evolutionary shifts in specificity.

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Section: Resultsmentioning

confidence: 73%

Transient Duplication-Dependent Divergence and Horizontal Transfer Underlie the Evolutionary Dynamics of Bacterial Cell–Cell Signaling

et al. 2016

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“…LuxS , a QS system best characterized in G- bacteria that uses AHLs as signaling molecules, plays a role in bacterial growth, and this system affects the expression of virulence factors in B. anthracis but its loss does not affect virulence [160–162]. In addition to the luxS system, numerous members of the Bacillus genus express members of the RNPP (Rap, NprR, PlcR, and PrgX) family of quorum sensing operons [56,60,163]. RNPP and agr share several crucial features, as both QS systems encode for a propeptide that is cleaved and secreted, and in the case of many RNPP members, the quormone is a heptapeptide [61,164].…”

Section: Agr and Analogous Peptide Quormone Systemsmentioning

confidence: 99%

Targeting agr- and agr-Like Quorum Sensing Systems for Development of Common Therapeutics to Treat Multiple Gram-Positive Bacterial Infections

Gray

Hall

Gresham

2013

Sensors

102

106

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Invasive infection by the Gram-positive pathogen Staphylococcus aureus is controlled by a four gene operon, agr that encodes a quorum sensing system for the regulation of virulence. While agr has been well studied in S. aureus, the contribution of agr homologues and analogues in other Gram-positive pathogens is just beginning to be understood. Intriguingly, other significant human pathogens, including Clostridium perfringens, Listeria monocytogenes, and Enterococcus faecalis contain agr or analogues linked to virulence. Moreover, other significant human Gram-positive pathogens use peptide based quorum sensing systems to establish or maintain infection. The potential for commonality in aspects of these signaling systems across different species raises the prospect of identifying therapeutics that could target multiple pathogens. Here, we review the status of research into these agr homologues, analogues, and other peptide based quorum sensing systems in Gram-positive pathogens as well as the potential for identifying common pathways and signaling mechanisms for therapeutic discovery.

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“…Alternatively, some linear-peptide-based QS systems actively transport the autoinducers back into the cell where the peptide signal can interact directly with a cognate regulator to alter target gene expression. Such is the case for the PrgX system of E. facaelis (20) and the PlcR and NprR systems of Bacillus thuringiensis (20,24,25) (Fig. 2).…”

Section: Peptide-based Quorum Sensingmentioning

confidence: 99%

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

LaSarre

Federle

2013

Microbiol Mol Biol Rev

676

556

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SUMMARY Cell-cell communication, or quorum sensing, is a widespread phenomenon in bacteria that is used to coordinate gene expression among local populations. Its use by bacterial pathogens to regulate genes that promote invasion, defense, and spread has been particularly well documented. With the ongoing emergence of antibiotic-resistant pathogens, there is a current need for development of alternative therapeutic strategies. An antivirulence approach by which quorum sensing is impeded has caught on as a viable means to manipulate bacterial processes, especially pathogenic traits that are harmful to human and animal health and agricultural productivity. The identification and development of chemical compounds and enzymes that facilitate quorum-sensing inhibition (QSI) by targeting signaling molecules, signal biogenesis, or signal detection are reviewed here. Overall, the evidence suggests that QSI therapy may be efficacious against some, but not necessarily all, bacterial pathogens, and several failures and ongoing concerns that may steer future studies in productive directions are discussed. Nevertheless, various QSI successes have rightfully perpetuated excitement surrounding new potential therapies, and this review highlights promising QSI leads in disrupting pathogenesis in both plants and animals.

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Evolution and some functions of the NprR–NprRB quorum-sensing system in the Bacillus cereus group

Cited by 28 publications

References 37 publications

Transient Duplication-Dependent Divergence and Horizontal Transfer Underlie the Evolutionary Dynamics of Bacterial Cell–Cell Signaling

Transient Duplication-Dependent Divergence and Horizontal Transfer Underlie the Evolutionary Dynamics of Bacterial Cell–Cell Signaling

Targeting agr- and agr-Like Quorum Sensing Systems for Development of Common Therapeutics to Treat Multiple Gram-Positive Bacterial Infections

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

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