Evolution and molecular mechanism of PitAs in iron transport of Streptococcus species

Crizotinib Shows Antibacterial Activity against Gram-Positive Bacteria by Reducing ATP Production and Targeting the CTP Synthase PyrG

“…The positive clones were screened with a 4 mg/L chloramphenicol-resistant blood agar plate. The detailed procedures of gene knockout and complementation were the same as that described in our previous studies ( 34 ). The primers are listed in Table 1 .…”

“…The last 50-ns trajectory of PyrG, PyrG G18A K39A E141A D305A , and PyrG-crizotinib complex was extracted to visualize the conformation change during MDS. GraphPad Prism 8.0.2 software were used to draw the structure diagram ( 34 ).…”

Identification and Tetramer Structure of Hemin-Binding Protein SPD_0310 Linked to Iron Homeostasis and Virulence of Streptococcus pneumoniae

“…In S. pneumoniae , three different iron uptake systems have evolved that promote effective absorption of iron sources. These are the pneumococcal iron ABC transporter (PitA) ( 13 , 14 ), pneumococcal iron uptake (PiuA) ( 15 , 16 ), and pneumococcal iron acquisition ATP-binding cassette (PiaA) ( 17 ), for Fe 3+ , hemin, and ferrichrome, respectively. GAPDH of S. pneumoniae is responsible for competing for hemin via interacting with human hemoglobin and plasminogen ( 6 , 18 ).…”

“…SPD_0310 is a cytosolic protein without a signal peptide and is defined as a hypothetical protein belonging to the UPF0371 family in the NCBI database. Several studies by our group have shown that this protein may participate in metal binding ( 14 , 20 , 21 ). First, iTRAQ-based proteomics have revealed that compared to wild-type S. pneumoniae D39 (WT-D39), the expression level of SPD_0310 protein increased approximately 3.5-fold in Δ piaA Δ piuA Δ pitA (triple mutant), in which three major iron transporters were simultaneously deleted ( 20 , 22 ).…”

“…First, iTRAQ-based proteomics have revealed that compared to wild-type S. pneumoniae D39 (WT-D39), the expression level of SPD_0310 protein increased approximately 3.5-fold in Δ piaA Δ piuA Δ pitA (triple mutant), in which three major iron transporters were simultaneously deleted ( 20 , 22 ). Second, SPD_0310 interacts with the intracellular iron transporters SPD_0226 and SPD_0227 of S. pneumoniae ( 14 ). Third, the expression level of SPD_0310 in S. pneumoniae in manganese-deficient medium was 4.58-fold higher ( P < 0.05) than that in normal medium ( 21 ).…”