Evolution and molecular basis of substrate specificity in a 3-ketoacyl-CoA synthase gene cluster from Populus trichocarpa

Chen, Jeff Y.; Mumtaz, Arishba; Gonzales‐Vigil, Eliana

doi:10.1016/j.jbc.2022.102496

Cited by 7 publications

(3 citation statements)

References 66 publications

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“…The GPAT gene is associated with fatty acid unsaturation and plays a pivotal role in cold resistance in a number of plant species. , Plant 3-ketoacyl-CoA synthase (KCS), which is involved in the biosynthesis of very long chain fatty acids (VLCFAs), was increased 20-fold upon SLT stress training (Figure S5D). VLCFAs play important roles in biosynthesis of cuticular waxes, a hydrophobic film deposited on aerial tissue surfaces, and provides protection against pathogen infection . In addition, lipid-associated plant defense responses are largely facilitated by the activation of lipases (lipid hydrolyzing proteins), which cleave or transform lipid substrates in various subcellular compartments .…”

Section: Resultsmentioning

confidence: 99%

“…VLCFAs play important roles in biosynthesis of cuticular waxes, a hydrophobic film deposited on aerial tissue surfaces, and provides protection against pathogen infection. 51 In addition, lipid-associated plant defense responses are largely facilitated by the activation of lipases (lipid hydrolyzing proteins), which cleave or transform lipid substrates in various subcellular compartments. 52 We found that lipases were highly expressed in rice leaves with SLT training (Figure S5D).…”

Section: Acsmentioning

confidence: 99%

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Engineering Climate-Resilient Rice Using a Nanobiostimulant-Based “Stress Training” Strategy

et al. 2023

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Under a changing climate, cultivating climate-resilient crops will be critical to maintaining food security. Here, we propose the application of reactive oxygen species (ROS)-generating nanoparticles as nanobiostimulants to trigger stress/immune responses and subsequently increase the stress resilience of plants. We established three regimens of silver nanoparticles (AgNPs)-based “stress training”: seed training (ST), leaf training (LT), and combined seed and leaf training (SLT). Trained rice seedlings were then exposed to either rice blast fungus (Magnaporthe oryzae) or chilling stress (10 °C). The results show that all “stress training” regimes, particularly SLT, significantly enhanced the resistance of rice against the fungal pathogen (lesion size reduced by 82% relative to untrained control). SLT also significantly enhanced rice tolerance to cold stress. The mechanisms for the enhanced resilience were investigated with metabolomics and transcriptomics, which show that “stress training” induced considerable metabolic and transcriptional reprogramming in rice leaves. AgNPs boosted ROS-activated stress signaling pathways by oxidative post-translational modifications of stress-related kinases, hormones, and transcriptional factors (TFs). These signaling pathways subsequently modulated the expression of defense genes, including specialized metabolites (SMs) biosynthesis genes, cell membrane lipid metabolism genes, and pathogen–plant interaction genes. Importantly, results showed that the “stress memory” can be transferred transgenerationally, conferring offspring seeds with improved seed germination and seedling vigor. This may provide an epigenetic breeding strategy to fortify stress resilience of crops. This nanobiostimulant-based stress training strategy will increase yield vigor against a changing climate and will contribute to sustainable agriculture by reducing agrochemical use.

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Section: Resultsmentioning

confidence: 99%

Section: Acsmentioning

confidence: 99%

Engineering Climate-Resilient Rice Using a Nanobiostimulant-Based “Stress Training” Strategy

et al. 2023

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show abstract

“…Plant 3-ketoacyl-CoA synthase (KCS), which is involved in the biosynthesis of very long chain fatty acids (VLCFAs) 50 , was increased 20-fold upon SLP stress training (Figure S5D). VLCFAs play important roles in biosynthesis of cuticular waxes, a hydrophobic lm deposited on aerial tissue surfaces and provides protection against pathogen infection 51 . In addition, lipid-associated plant defense responses are largely facilitated by the activation of lipases (lipid hydrolyzing proteins), which cleave or transform lipid substrates in various subcellular compartments 52 .…”

Section: Transcriptomic Reprogramming In Rice Leavesmentioning

confidence: 99%

Engineering Climate-Resilient Rice Using a Nanobiostimulant-based “Stress Training” Strategy

Chen

Pan

Zhao

et al. 2023

Preprint

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Under a changing climate, cultivating climate-resilient crops will be critical to maintaining food security. Here, we propose the application of ROS-generating nanoparticles as nanobiostimulants to trigger stress/immune responses, and subsequently increase the stress resilience of plants. We established three regimens of AgNPs-based “stress training”: seed priming (SP), leaf priming (LP), and combined seed- and leaf- priming (SLP). Trained rice seedlings were then exposed to either rice blast fungus (M. oryzae.) or chilling stress (10 ºC). The results show that all “stress training” regimes, particularly SLP significantly enhanced the resistance of rice against the fungal pathogen (lesion size reduced by 82% relative to un-trained control). SLP training also significantly enhanced rice tolerance to cold stress. Under cold conditions, SLP training significantly increased leaf biomass by 35% compared to controls. The mechanisms for the enhanced resilience were investigated with metabolomic and transcriptomic profiling, which show that “stress training” induced considerable metabolic and transcriptional reprogramming in rice leaves. AgNPs-boosted ROS activated stress signaling pathways by oxidative post-translational modifications of stress related kinases, hormones, and transcriptional factors (TFs). These signaling pathways subsequently modulated the expression of defense genes, including specialized metabolites (SMs) biosynthesis genes, cell membrane lipid metabolism genes, and pathogen-plant interaction genes. These AgNPs-triggered metabolic and transcriptional reprogramming enable rice plants to mount a more rapid and intense response to future stresses. This nanobiostimulant-based strategy for increasing the stress resilience of crops will increase yield vigor against a changing climate and will contribute to sustainable agriculture by reducing agrochemical use.

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Peeling off the Poplar Cuticle, from Biochemistry to Defensive Roles

Chen,

Zamani-Babgohari,

Gonzales-Vigil

2024

Compendium of Plant Genomes

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Evolution and molecular basis of substrate specificity in a 3-ketoacyl-CoA synthase gene cluster from Populus trichocarpa

Cited by 7 publications

References 66 publications

Engineering Climate-Resilient Rice Using a Nanobiostimulant-Based “Stress Training” Strategy

Engineering Climate-Resilient Rice Using a Nanobiostimulant-Based “Stress Training” Strategy

Engineering Climate-Resilient Rice Using a Nanobiostimulant-based “Stress Training” Strategy

Peeling off the Poplar Cuticle, from Biochemistry to Defensive Roles

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