Evolution and expression of FOXL2

Cocquet, Julie; Pailhoux, Eric; Jaubert, Françis; Servel, Nathalie; Xia, Xuhua; Pannetier, Maëlle; Baere, Elfride De; Messiaen, Ludwine; Cotinot, Corinne; Fellous, Marc; Veitia, Reiner A.

doi:10.1136/jmg.39.12.916

Cited by 255 publications

(203 citation statements)

References 15 publications

(20 reference statements)

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“…As in goats ovaries (Cocquet et al, 2002), cFoxL2 protein was detected during development only in the somatic cells but not in oocytes. However, we demonstrated that cFoxL2 transcripts are highly expressed in maturing and ovulated oocytes from adult hens.…”

Section: Foxl2 and Later Developmentmentioning

confidence: 79%

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Isolation of chicken homolog of the FOXL2 gene and comparison of its expression patterns with those of aromatase during ovarian development

Govoroun

Pannetier

Pailhoux

et al. 2004

Developmental Dynamics

187

119

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Mutations in the forkhead transcription factor gene FOXL2 are involved in ovarian failure, which occurs in human BPES syndrome. This syndrome presents a sexually dimorphic expression, specific to the ovary in several vertebrates. We cloned the open reading frame of chicken FOXL2 (cFoxL2) and studied cFoxL2 expression in developing gonads and during adulthood to examine the role of FOXL2 in ovarian differentiation and function in birds. The spatial and temporal dynamics of cFoxL2 and aromatase expression were analyzed in parallel by using real-time quantitative reverse transcriptase-polymerase chain reaction and immunohistochemistry in attempt to investigate the possible role of cFoxL2 in the regulation of aromatase. The expression patterns of cFoxL2 and aromatase transcripts were highly correlated during the sex-differentiation period (4.7-12.7 days of incubation). Aromatase and cFoxL2 proteins were colocalized in the medullar part of female gonads on embryonic day 14. Fourteen days after hatching, cFoxL2 protein was mainly detected in granulosa cells of developing follicles. In adult ovary follicular envelopes, apart from granulosa cells, cFoxL2 transcript and protein were detected at lower levels in theca cells where aromatase was present. A high level of cFoxL2 transcription was also observed in maturing and ovulated oocytes. Our results confirm that FoxL2 is an early regulator of ovarian development in birds and may be involved in aromatase transcription regulation. Developmental Dynamics 231:859 -870, 2004.

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Section: Foxl2 and Later Developmentmentioning

confidence: 79%

“…This previously characterized polyclonal antibody detects a specific 45-kDa band on human and goat ovary protein extracts (Cocquet et al, 2002). First, Western blot immunoassay was performed to study the capacity and specificity of the antiserum to bind cFoxL2.…”

Section: Western Blot Immunoassay and Immunohistochemistrymentioning

confidence: 99%

Isolation of chicken homolog of the FOXL2 gene and comparison of its expression patterns with those of aromatase during ovarian development

Govoroun

Pannetier

Pailhoux

et al. 2004

Developmental Dynamics

187

119

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“…In mammals, Foxl2 is highly expressed in the ovary and is commonly regarded as female-specific (Cocquet et al 2002, Loffler et al 2003. In birds, expression of Foxl2 is predominantly observed in the ovary (Govoroun et al 2004).…”

Section: Sequence Analysis and Tissue Distribution Of Dmrt1s Foxl2 mentioning

confidence: 99%

Molecular cloning of doublesex and mab-3-related transcription factor 1, forkhead transcription factor gene 2, and two types of cytochrome P450 aromatase in Southern catfish and their possible roles in sex differentiation

Liu¹,

Wu²,

Jiao³

et al. 2007

Journal of Endocrinology

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To address the roles of doublesex and mab-3-related transcription factor 1 (Dmrt1), forkhead transcription factor gene 2 (Foxl2), and aromatase in sex differentiation of Southern catfish, the cDNA sequences of these genes were isolated from the gonads. Dmrt1a and Dmrt1b were found to be expressed in the gonads, being higher in the testis. A low expression level of Dmrt1b was also detected in the intestine and kidney of the male. Foxl2 was found to be expressed extensively in the brain (B), pituitary (P), gill and gonads (G), with the highest level in the ovary, indicating the possible involvement of Foxl2 in the B-P-G axis. Cytochrome P450 (Cyp)19b was found to be expressed in the brain, spleen, and gonads, while Cyp19a was only expressed in the gonads and spleen. All-female Southern catfish fry were treated with fadrozole (F), tamoxifen (TAM), and 17b-estradiol (E 2 ) respectively, from 5 to 25 days after hatching (dah).The expression levels of these genes were measured at 65 dah. In the F-, TAM-, and FCTAM-treated groups, Dmrt1a and Dmrt1b were up-regulated in the gonad, whereas Foxl2 and Cyp19a were down-regulated, while the expression of Cyp19b in the gonad remained unchanged. Furthermore, downregulation of Foxl2 and Cyp19b was also detected in the brain.In the E 2 -treated group, Dmrt1a and Dmrt1b were downregulated to an undetectable level in the gonad, whereas Foxl2 and Cyp19b were up-regulated in the brain. Consistent with the observed changes in the expressions of these genes, 56, 70, and 80% sex-reversed male individuals were obtained in the F-, TAM-, and FCTAM-treated groups respectively. These results indicate the significant roles of Dmrt1, Foxl2, and Cyp19 in the sex differentiation of Southern catfish.

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“…Its sexually dimorphic expression in embryonic ovaries of humans, ruminants, mice, chickens, and turtles, representatives of phylogenetically distant vertebrate groups, is consistent with a crucial role of FOXL2 in the ovarydifferentiation pathway [3,4,7,10]. In addition, mutations in the FOXL2 gene are responsible for BPES, a complex human disorder characterized primarily by malformations of the eyelids and other facial anomalies.…”

Section: Replacement Of the Pis-regulated Genes In The Gonad-differenmentioning

confidence: 57%

“…The expression levels of the two genes are higher in ovaries than in the testes of normal fetuses. Using FOXL2-specific antibodies, the protein has been investigated in the gonads of both sexes and has only been detected in the ovaries from 36 dpc to adulthood [3]. In XX PIS −/− sex-reversed gonads, transcription levels were greatly reduced as early as 36 dpc for FOXL2 (Fig.…”

Section: Pis Is a Transcriptional Regulatory Regionmentioning

confidence: 99%

Positional cloning of the PIS mutation in goats and its impact on understanding mammalian sex-differentiation

Pailhoux¹,

Vigier²,

Schibler

et al. 2005

Genet. Sel. Evol.

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-In goats, the PIS (polled intersex syndrome) mutation is responsible for both the absence of horns in males and females and sex-reversal affecting exclusively XX individuals. The mode of inheritance is dominant for the polled trait and recessive for sex-reversal. In XX PIS −/− mutants, the expression of testis-specific genes is observed very precociously during gonad development. Nevertheless, a delay of 4-5 days is observed in comparison with normal testis differentiation in XY males. By positional cloning, we demonstrate that the PIS mutation is an 11.7-kb regulatory-deletion affecting the expression of two genes, PISRT1 and FOXL2 which could act synergistically to promote ovarian differentiation. The transcriptional extinction of these two genes leads, very early, to testis-formation in XX homozygous PIS −/− mutants. According to their expression profiles and bibliographic data, we propose that FOXL2 may be an ovary-differentiating gene, and the non-coding RNA PISRT1, an anti-testis factor repressing SOX9, a key regulator of testis differentiation. Under this hypothesis, SRY, the testis-determining factor would inhibit these two genes in the gonads of XY males, to ensure testis differentiation. PIS mutation / goat / gonad differentiation / XX sex-reversal / ovary development

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Evolution and expression of FOXL2

Cited by 255 publications

References 15 publications

Isolation of chicken homolog of the FOXL2 gene and comparison of its expression patterns with those of aromatase during ovarian development

Isolation of chicken homolog of the FOXL2 gene and comparison of its expression patterns with those of aromatase during ovarian development

Molecular cloning of doublesex and mab-3-related transcription factor 1, forkhead transcription factor gene 2, and two types of cytochrome P450 aromatase in Southern catfish and their possible roles in sex differentiation

Positional cloning of the PIS mutation in goats and its impact on understanding mammalian sex-differentiation

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