Endocannabinoids (eCB) are key regulators of excitatory/ inhibitory neurotransmission at cannabinoid-1-receptor (CB 1 R)-expressing axon terminals. The most abundant eCB in the brain, that is 2-arachidonoylglycerol (2-AG), is hydrolyzed by the enzyme monoacylglycerol lipase (MAGL), whose chronic inhibition in the brain was reported to cause CB 1 R desensitization. We employed the MAGL knock-out mouse (MAGLÀ/À), a genetic model of congenital and sustained elevation of 2-AG levels in the brain, to provide morphological and biochemical evidence for b-arrestin2-mediated CB 1 R desensitization in brain regions involved in the control of emotional states, that is, the prefrontal cortex (PFC), amygdala, hippocampus and cerebellar cortex. We found a widespread CB 1 R/b-arrestin2 co-expression in the mPFC, amygdala and hippocampus accompanied by impairment of extracellular signal-regulated kinase signaling and elevation of vesicular glutamate transporter (VGluT1) at CB 1 R-positive excitatory terminals in the mPFC, or vesicular GABA transporter (VGAT) at CB 1 R-positive inhibitory terminals in the amygdala and hippocampus. The impairment of CB 1 R signaling in MAGLÀ/À mice was also accompanied by enhanced excitatory drive in the basolateral amygdala (BLA)-mPFC circuit, with subsequent elevation of glutamate release to the mPFC and anxiety-like and obsessive-compulsive behaviors, as assessed by the light/dark box and marble burying tests, respectively. Collectively, these data provide evidence for a barrestin2-mediated desensitization of CB 1 R in MAGLÀ/À mice, with impact on the synaptic plasticity of brain circuits involved in emotional functions.