Evident variations of fungal and actinobacterial cellulolytic communities associated with different humified particle-size fractions in a long-term fertilizer experiment

Zhang, Qian; Liang, Guozheng; Guo, Tengfei; He, Ping; Wang, Xiu‐Bin; Zhou, Wei

doi:10.1016/j.soilbio.2017.05.022

Cited by 45 publications

(35 citation statements)

References 58 publications

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“…However, our data showed that F/B increased with SND levels, indicating that bacterial development was limited by the SND levels and that fungi increased the destruction of high-resistance carbon complexes [27]. This may be related to the time of SND and the background pH of the soil, as prior reports have argued that long-term N deposition had a greater negative impact on fungal biomass than that of bacteria, resulting in a decline in F/B [29,45,55]. Hence, after a short-term SND, soil microorganisms may still exist during the stress and adjustment phase, as indicated by the difference in the microbial trend of responses observed in this study compared to those observed for the long-term N deposition.…”

Section: Microbial Community Composition and Structurecontrasting

confidence: 38%

“…Particle-size fractionation, which allows the separation of soil organic matter with varying degrees of microbial alteration, might help elucidate microbe-mediated soil carbon (C) and N cycling characteristics [28,29]. Microbial acquisition of a substrate is compounded by relatively stable C and N levels [30], high nutrient and O 2 availability [31], and adsorption of extracellular enzymes to the clay and silt fractions but not to their aggregates [20].…”

Section: Introductionmentioning

confidence: 99%

“…Microbial acquisition of a substrate is compounded by relatively stable C and N levels [30], high nutrient and O 2 availability [31], and adsorption of extracellular enzymes to the clay and silt fractions but not to their aggregates [20]. As soil changes induced by N deposition are mainly the result of microbiological processes, different substrates and soil properties are selected by specifically adapted microbial communities [18,29]. A prior study demonstrated that changes in microbial communities depend on their microhabitats, with a greater abundance of bacterial and fungal genes observed in the soil fractions with larger-sized particles (>63 µm) compared to silt (2-20 µm) [28].…”

Section: Introductionmentioning

confidence: 99%

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Variations in the Compositions of Soil Bacterial and Fungal Communities Due to Microhabitat Effects Induced by Simulated Nitrogen Deposition of a Bamboo Forest in Wetland

Huang²,

Ekawati

et al. 2019

Forests

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Although numerous studies have been published on nitrogen (N) deposition, little is known about its impact on microbial communities in wetland forests. Here, we used simulated nitrogen deposition (SND) to analyze the importance of differences in soil microhabitats in promoting the diversity of soil bacteria and fungi. We compared various levels of SND (control (CK), low N (N30), medium N (N60), and high N (N90)) and found that these were associated with changes in soil microhabitats. Additionally, SND affected soil pH, clay and sand content of the soil, and specific surface area (SSA). Bacteria and fungi responded differently to increased SND levels. The alpha diversity of bacteria decreased with an increased SND level, while fungal abundance, diversity, and community evenness reached their maximum values at the N60 threshold. Principal coordinates analysis (PCoA), nonparametric multivariate analysis of variance (PERMANOVA), and linear discriminant analysis (LDA) coupled with effect size measurements (LefSe) also confirmed that the bacterial composition was different at N90 compared to other levels of SND while that of fungi was different at N60. A higher discriminant level (LDA score ≥4) may be a valuable index of selecting indicator microbial clades sensitive to SND for wetland management. Further, an increased pH was associated with a greater abundance of bacteria and fungi. In addition, the volume contents of clay and SSA were negatively correlated with bacteria but fungi are associated with soil specific gravity (SSG). Overall, in a neutral soil pH environment, pH fluctuation is the main influencing factor in terms of bacterial and fungal abundance and diversity. The diversity of fungi is more dependent on the type and relative content of solid phase components in soil than that of bacteria, implying the presence of species-specific niches for bacteria and fungi. These results demonstrate that changes in SND can induce short-term microbial and microhabitat changes.

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Section: Microbial Community Composition and Structurecontrasting

confidence: 38%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Variations in the Compositions of Soil Bacterial and Fungal Communities Due to Microhabitat Effects Induced by Simulated Nitrogen Deposition of a Bamboo Forest in Wetland

Huang²,

Ekawati

et al. 2019

Forests

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“…The degree of aliphaticity and aromaticity of the HAs were calculated as below (Canellas et al, 2012; Zhang, Liang et al, ):

Aliphaticity ((), AP) = ((), δ 0 \sim 110) / ((), δ 0 \sim 160);

Aromaticity ((), AR) = ((), δ 110 \sim 160) / ((), δ 0 \sim 160) .

…”

Section: Methodsmentioning

confidence: 99%

“…The degree of aliphaticity and aromaticity of the HAs were calculated as below (Canellas et al, 2012;Zhang, Liang et al, 2017):…”

Section: Solid-state 13 C Nmr Spectroscopymentioning

confidence: 99%

Long‐term mineral fertilization in paddy soil alters the chemical structures and decreases the fungistatic activities of humic acids

Wei

Liu

et al. 2019

European J Soil Science

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Fungistatic activity is a newly identified ecological function for soil humic acids. However, the relation between fungistatic activities of soil humic acids and their chemical structures is still unclear. In addition, how fertilizer application affects fungistatic activity is still unknown. We sampled paddy soils with different long‐term fertilizer schemes, namely: unfertilized control (CK), mineral fertilizer applied (NPK) and mineral fertilizer applied plus straw return (NPKRS). Soil humic acids were extracted and their fungistatic activities against 14 phytopathogenic fungi were investigated to evaluate their ecological functions. The humic acids from NPK and NPKRS treatments showed much smaller fungistatic effects than those from CK treatments. The chemical structures of humic acids were investigated by 13C nuclear magnetic resonance (13C NMR) spectroscopy. Long‐term fertilization significantly changed the chemical structure of soil humic acid, and the variation in chemical structure affected the fungistatic activities. The relative content of alkoyl C and alkyl C, the alkoyl C/alkyl C ratio and aliphaticity all had positive correlations with the fungistatic effects of humic acids. That indicated humic acids with more aliphatic and decomposable structures showed more fungistatic activity. From the results, we believe that long‐term application of mineral fertilizer in paddy fields makes the soil humic acids less aliphatic and more recalcitrant, and concomitantly weakens their ability to inhibit phytopathogenic fungi. Highlights We studied the inhibitory effects of paddy soil humic acids on 14 phytopathogenic fungi. Fertilization‐induced changes in humic acids affect inhibition of phytopathogenic fungi. Long‐term mineral fertilization significantly changed chemical structures of humic acids. Humic acid with more aliphatic and decomposable structures had more fungistatic activity.

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Active chemical fractions with high fungistatic activity of humic‐like substances obtained via different extraction solvents

Wei

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND: Humic-like substances (HULIS) for use in controlling soil-borne diseases have been a research focus, but obtaining HULIS with high fungistatic activity via different extraction solvents has been challenging. In this work, three solvents were selected to extract HULIS from weathered coal. Inhibition rates against phytopathogens were used to evaluate the fungistatic activities of HULIS. Element contents (C, N, S, and O) and carbon-13-cross polarization magic-angle spinning nuclear magnetic resonance ( 13 C-CP-MAS NMR) spectra were used to evaluate the chemical composition. The relationships between the chemical compositions and fungistatic activities were assessed by redundancy analysis (RDA) and partial least squares regression (PLSR). RESULTS: The results showed that HULIS extracted with different solvents varied in the element contents, organic carbon structures and fungistatic activities. HULIS extracted with potassium hydroxide-ethanol had the highest content of C, the lowest contents of N and O, and the highest fungistatic activities (up to 71.86%). Consistent results through RDA and PLSR shows that C and S had positive effects on the fungistatic activity, while N and O had negative effects. Aliphatic C in HULIS had positive effects on the fungistatic activity, while the aromatic fraction and anomeric C had negative effects.CONCLUSION: In summary, different solvents extracted different fractions from weather coal. Mixed solvents with organic and inorganic solvents were more beneficial for extracting active fractions with higher fungistatic activity of HULIS from weather coal. The results of this work provide a good theoretical basis for the application of HULIS extracted from weathered coal. Fungistatic activityThe inhibition rates of HULIS against 13 phytopathogenic fungi were measured, and the results are summarized in Table 1. For J Chem Technol Biotechnol 2020; 95: 585-593

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Evident variations of fungal and actinobacterial cellulolytic communities associated with different humified particle-size fractions in a long-term fertilizer experiment

Cited by 45 publications

References 58 publications

Variations in the Compositions of Soil Bacterial and Fungal Communities Due to Microhabitat Effects Induced by Simulated Nitrogen Deposition of a Bamboo Forest in Wetland

Variations in the Compositions of Soil Bacterial and Fungal Communities Due to Microhabitat Effects Induced by Simulated Nitrogen Deposition of a Bamboo Forest in Wetland

Long‐term mineral fertilization in paddy soil alters the chemical structures and decreases the fungistatic activities of humic acids

Active chemical fractions with high fungistatic activity of humic‐like substances obtained via different extraction solvents

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