Evidence that POU factor brn-3B regulates expression ofPax-6 in neuroretina cells

Plaza, Serge; Hennemann, Hanjo; Möröy, Tarik; Saule, Simon; Dozier, Christine

doi:10.1002/(sici)1097-4695(19991115)41:3<349::aid-neu4>3.0.co;2-f

Cited by 9 publications

(12 citation statements)

References 35 publications

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“…For example, Trieu et al (1999) found that Brn3b can modestly activate transcription from the brn3a promoter by binding to Brn3 consensus sites within the promoter region. Similar results showing Brn3b's activation properties have been reported by others (Plaza et al, 1999; Liu et al, 2000). In contrast, Latchman and his co‐workers (Budhram‐Mahadeo et al, 1995, 1999; Morris et al, 1996) have provided evidence that Brn3b can repress transcription from several promoters, including the α‐internexin promoter, the SNAP‐25 promoter, and the BRCA1 promoter.…”

Section: Introductionsupporting

confidence: 91%

Identification of an N-terminal transcriptional activation domain within Brn3b/POU4f2

Martin

Klein³

2005

Differentiation

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Section: Introductionsupporting

confidence: 91%

Identification of an N-terminal transcriptional activation domain within Brn3b/POU4f2

Martin

Klein³

2005

Differentiation

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“…Some examples include Pax6, Nf66, Snap25, and Scg10. Pax6, which is expressed in RGCs at E14.5, has been reported to be a potential target of Brn3b (Plaza et al, 1999). Our results suggest that Brn3b is not essential for Pax6 expression.…”

Section: Discussionmentioning

confidence: 56%

“…Furthermore, many genes that are directly regulated by Brn3b probably have promoter/enhancer elements that use other transcription factors in addition to Brn3b, including those transcription factors identified as Brn3b dependent. The transcriptional activity of Brn3b has not been clearly defined, although it appears that Brn3b is capable of transcriptional activation or repression, depending on the cellular and promoter context (Budhram-Mahadeo et al, 1999;Plaza et al, 1999). When measured by real-time PCR, up-or downregulation of Brn3b-dependent genes ranged from twice the value observed in wild-type RNA to more than 40 times, suggesting that a variety of transcriptional readout mechanisms exist that rely partly or completely on Brn3b.…”

Section: Discussionmentioning

confidence: 99%

Discrete gene sets depend on POU domain transcription factor Brn3b/Brn-3.2/POU4f2 for their expression in the mouse embryonic retina

et al. 2004

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Brn3b/Brn-3.2/POU4f2 is a POU domain transcription factor that is essential for retinal ganglion cell (RGC) differentiation, axonal outgrowth and survival. Our goal was to establish a link between Brn3b and the downstream events leading to RGC differentiation. We sought to determine both the number and types of genes that depend on Brn3b for their expression. RNA probes from wild-type and Brn3b-/- E14.5, E16.5 and E18.5 mouse retinas were hybridized to a microarray containing 18,816 retina-expressed cDNAs. At E14.5, we identified 87 genes whose expression was significantly altered in the absence of Brn3b and verified the results by real-time PCR and in situ hybridization. These genes fell into discrete sets that encoded transcription factors, proteins associated with neuron integrity and function,and secreted signaling molecules. We found that Brn3b influenced gene expression in non RGCs of the retina by controlling the expression of secreted signaling molecules such as sonic hedgehog and myostatin/Gdf8. At later developmental stages, additional alterations in gene expression were secondary consequences of aberrant RGC differentiation caused by the absence of Brn3b. Our results demonstrate that a small but crucial fraction of the RGC transcriptome is dependent on Brn3b. The Brn3b-dependent gene sets therefore provide a unique molecular signature for the developing retina.

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“…For example, during differentiation of rat cultured dorsal root ganglion cells, the Brn‐3b short isoform is down‐regulated, whereas the long isoform is up‐regulated (Liu et al, 1996). In the developing quail retina, only the long brn‐3b transcript has been detected, but the expression of the short form was not assayed later in development (Plaza et al, 1999). Our data show a temporal difference in the expression of the long and short brn‐3b isoforms in the zebrafish.…”

Section: Resultsmentioning

confidence: 99%

Developmental expression of the POU domain transcription factor Brn‐3b (Pou4f2) in the lateral line and visual system of zebrafish

deCarvalho

Cappendijk

Fadool

2004

Developmental Dynamics

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Members of the class IV POU domain transcription factors are important regulators of neural development. In mouse, Brn-3b (Pou4f2, Brn3.2) and Brn-3c (Pou4f3, Brn3.1) are essential for the normal differentiation and maturation of retinal ganglion cells (RGCs) and hair cells of the auditory system, respectively. In this report, the cloning and expression profile of brn-3b in the zebrafish (Danio rerio) were assessed as the first step for understanding its role in the development of sensory systems. Two brn-3b alternative transcripts exhibited different onset of expression during development but shared overlapping expression domains in the adult visual system. The brn-3b expression in the zebrafish retina was consistent with a conserved role in differentiation and maintenance of RGCs. Expression was also observed in the optic tectum. Unexpectedly, brn-3b was prominently expressed in the migrating posterior lateral line primordium and larval neuromasts.

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Evidence that POU factor brn-3B regulates expression ofPax-6 in neuroretina cells

Cited by 9 publications

References 35 publications

Identification of an N-terminal transcriptional activation domain within Brn3b/POU4f2

Identification of an N-terminal transcriptional activation domain within Brn3b/POU4f2

Discrete gene sets depend on POU domain transcription factor Brn3b/Brn-3.2/POU4f2 for their expression in the mouse embryonic retina

Developmental expression of the POU domain transcription factor Brn‐3b (Pou4f2) in the lateral line and visual system of zebrafish

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