Evidence That Peroxynitrite Affects Human Osteoblast Proliferation and Differentiation

Rocha, Francisco Airton Castro; Brum‐Fernandes, Artur J. de

doi:10.1359/jbmr.2002.17.3.434

Cited by 18 publications

(12 citation statements)

References 36 publications

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“…This is, to our knowledge, the first demonstration of an in vivo protective role for NO on articular cartilage during experimental arthritis. In accordance with these data, we have previously shown that NO is essential to the proliferation and differentiation of human osteoblasts in vitro and PN scavenging is protective to these cells (Da Rocha & De Brum‐Fernandes, 2002). It was also recently reported that NO by itself is protective to chondrocytes under oxidative stress in vitro , while reactive oxygen species, including PN, promote chondrocyte death (Del Carlo & Loeser, 2002).…”

Section: Discussionsupporting

confidence: 81%

“…Detection of 3‐nitrotyrosine in atherosclerotic plaques ( Beckman et al , 1994 ), in the serum and synovial fluid of rheumatoid arthritis patients (Kaur & Halliwell, 1994), and in rejected human kidney allografts ( Macmillan‐Crow et al , 1996 ) are some of the examples given as evidence of the association of PN formation with human disease states. In keeping with this finding, recent in vitro data have shown that NO is not damaging to either osteoblasts or chondrocytes in vitro when the generation of PN is inhibited (Da Rocha & De Brum‐Fernandes, 2002; Del Carlo & Loeser, 2002).…”

Section: Introductionsupporting

confidence: 66%

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Reactive nitrogen species scavenging, rather than nitric oxide inhibition, protects from articular cartilage damage in rat zymosan‐induced arthritis

Bezerra

Brain

Greenacre

et al. 2004

British J Pharmacology

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1 The contribution of nitric oxide (NO) and peroxynitrite (PN) to inflammation in a zymosaninduced (1 mg, intra-articular, i.art.) rat model of arthritis was assessed by histopathology and by measuring the glycosaminoglycan (GAG) content of the articular cartilage. 2 Progression of the chronic synovitis in zymosan-induced arthritis (ZYA) was associated with increased nitrite and nitrotyrosine (3-NT) levels in the joint exudates that paralleled a progressive loss of the GAG content. An increase in 3-NT was also observed after i.art. PN. 3 The nonselective nitric oxide synthase (NOS) inhibitor L-N G -nitroarginine methyl ester (25-75 mg kg À1 day À1 ) or the selective inducible NOS inhibitor aminoguanidine (50-100 mg kg À1 day À1 ) given 1 h before (prophylactic) or 3 days after (therapeutic) injection of the zymosan ameliorated the synovitis, but worsened the GAG loss, as measured at the end of the experiment (day 7). 4 The PN scavenger uric acid (100-250 mg kg À1 i.p. four times daily) given prophylactically until the end of the experiment (day 14), in a dose compatible with its PN scavenging activity, significantly decreased both the synovitis and the GAG loss. 5 In conclusion, PN formation is associated with cartilage damage in addition to proinflammatory activity in ZYA. NOS inhibitors and a PN scavenger were able to reduce the cellular infiltration, while displaying opposite effects on cartilage homeostasis either by enhancing or ameliorating the damage, respectively.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionsupporting

confidence: 66%

Reactive nitrogen species scavenging, rather than nitric oxide inhibition, protects from articular cartilage damage in rat zymosan‐induced arthritis

Bezerra

Brain

Greenacre

et al. 2004

British J Pharmacology

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“…For instance, NO per se is essential to normal osteoblast metabolism. On the other hand, peroxynitrite formed during inflammatory reactions by the combination of NO and superoxide can be deleterious to these cells (da Rocha and de Brum‐Fernandes, 2002). In addition, NO can have different effects depending on the tissue.…”

Section: Discussionmentioning

confidence: 99%

Tadalafil analgesia in experimental arthritis involves suppression of intra‐articular TNF release

Rocha

Silva

Leite

et al. 2011

British J Pharmacology

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BACKGROUND AND PURPOSEWe investigated the effect of the phosphodiesterase-5 inhibitor, tadalafil, on the acute hypernociception in rat models of arthritis. EXPERIMENTAL APPROACHRats were treated with either an intra-articular injection of zymosan (1 mg) or surgical transection of the anterior cruciate ligament (as an osteoarthritis model). Controls received saline intra-articular or sham operation respectively. Joint pain was evaluated using the articular incapacitation test measured over 6 h following zymosan or between 4 and 7 days after anterior cruciate ligament transection. Cell counts, tumour necrosis factor-a (TNF-a), interleukin-1 (IL-1), and the chemokine, cytokine-induced neutrophil chemoattractant-1 (CINC-1) were measured in joint exudates 6 h after zymosan. Groups received tadalafil (0.02-0.5 mg·kg -1 per os) or saline 2 h after intra-articular zymosan. Other groups received the m-opioid receptor antagonist naloxone or the cGMP inhibitor 1H-[1,2,4] oxadiazolo [4,3-a] quinoxalin-1-one (ODQ) before tadalafil. KEY RESULTSTadalafil dose-dependently inhibited hypernociception in zymosan and osteoarthritis models. In zymosan-induced arthritis, tadalafil significantly decreased cell influx and TNF-a release but did not alter IL-1 or CINC-1 levels. Pretreatment with ODQ but not with naloxone prevented the anti-inflammatory effects of tadalafil. CONCLUSIONS AND IMPLICATIONSTherapeutic oral administration of tadalafil provided analgesia mediated by guanylyl cyclase and was independent of the release of endogenous opioids. This effect of tadalafil was associated with a decrease in neutrophil influx and TNF-a release in inflamed joints.

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“…We detected reduced levels of NO in the joint exudates of animals treated with the A. suum extract. While NO per se appears to be beneficial to joint homeostasis (5,10,11), the combination of NO with other reactive species, such as the superoxide anion, may result in peroxynitrite formation and subsequent tissue lesion (23). We have recently demonstrated that neutrophils contribute to the formation of peroxynitrite in ZYA (4).…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of an Extract fromAscaris suumin Experimental Arthritis Models

Rocha¹,

Leite²,

Pompeu

et al. 2008

Infect Immun

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We investigated the effect of an extract from a helminth (Ascaris suum) in zymosan-induced arthritis (ZYA) or collagen-induced arthritis (CIA). Rats and mice, respectively, received 1 mg and 0.1 mg zymosan intraarticularly (i.a.). Test groups received an A. suum extract either per os (p.o.) or intraperitoneally (i.p.) 30 min prior to i.a. zymosan. Controls received saline. Hypernociception was measured using the articular incapacitation test. Cell influx, nitrite, and cytokine levels were assessed in joint exudates. The synovia and distal femoral extremities were used for histopathology. Cartilage damage was assessed through determining glycosaminoglycan (GAG) content. DBA/1J mice were subjected to CIA. The test group received A. suum extract i.p. 1 day after CIA became clinically detectable. Clinical severity and hypernociception were assessed daily. Neutrophil influx was determined using myeloperoxidase activity. The A. suum extract, either i.p. or p.o., significantly and dose-dependently inhibited cell influx and hypernociception in ZYA in addition to reducing GAG loss and ameliorating synovitis. The A. suum extract reduced i.a. levels of NO, interleukin-1␤ (IL-1␤), and IL-10 but not tumor necrosis factor alpha (TNF-␣) in rats subjected to ZYA while reducing i.a. IL-10, but not IL-1␤ or TNF-␣, levels in mice. Clinically, mice subjected to CIA treated with the A. suum extract had less severe arthritis. Hypernociception, myeloperoxidase activity, and synovitis severity were significantly reduced. These data show that a helminth extract given p.o. protects from arthritis severity in two classical arthritis models. This A. suum effect is species independent and functions orally and parenterally. The results show clinical and structural benefits when A. suum extract is given either prophylactically or therapeutically.

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Evidence That Peroxynitrite Affects Human Osteoblast Proliferation and Differentiation

Cited by 18 publications

References 36 publications

Reactive nitrogen species scavenging, rather than nitric oxide inhibition, protects from articular cartilage damage in rat zymosan‐induced arthritis

Reactive nitrogen species scavenging, rather than nitric oxide inhibition, protects from articular cartilage damage in rat zymosan‐induced arthritis

Tadalafil analgesia in experimental arthritis involves suppression of intra‐articular TNF release

Protective Effect of an Extract fromAscaris suumin Experimental Arthritis Models

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