Evidence that Listeria innocua modulates its membrane’s stored curvature elastic stress, but not fluidity, through the cell cycle

Furse, Samuel; Jakubec, Martin; Rise, Frode; Williams, Huw E. L.; Rees, Catherine; Halskau, Øyvind

doi:10.1038/s41598-017-06855-z

Cited by 32 publications

(78 citation statements)

References 79 publications

(75 reference statements)

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“…A combination of Direct Infusion Mass Spectrometry (DI-MS [27][28][29] ) and phosphorus nuclear magnetic resonance ( 31 P NMR 30,31 ), known as dual spectroscopy 29 , was used to identify and verify the abundance of lipid classes between the two ionisation modes respectively ( Fig. S1, NMR data for each compartment shown in Supplementary Information).…”

Section: Resultsmentioning

confidence: 99%

“…Samples were stored at -80 °C. Samples were dissolved in a modified 29,30 form of the 'CUBO' solvent system [45][46][47][48] (the amount of dueteriated dimethylformamide d7-DMF was minimised). Stock solutions of the solvent consisted of dimethylformamide (3·5 mL), d7-DMF (1·5 mL), triethylamine (1·5 mL) and guanidinium chloride (500 mg).…”

Section: Di-ms Data Processingmentioning

confidence: 99%

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Revealing grand-paternal programming of lipid metabolism using a novel computational tool

Furse

Watkins

Chiarugi

et al. 2020

Preprint

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Furse et al. Unravelling grand-paternal programming of lipid metabolism using novel lipid computational tool, 2020. P a g e | 1Abstract While the consequences of poor maternal diet on the offspring's cardio-metabolic health have been studied in detail, the role of the father's diet on the health of his offspring is poorly understood. We used a known mouse model to establish the impact of an isocaloric paternal low-protein high-carbohydrate diet on the offspring's lipid metabolism. Detailed lipid profiles were acquired from F1 neonate (3 weeks), F1 adult (16 weeks) and F2 neonate male and female offspring, in serum, liver, brain, heart and abdominal adipose tissues by Mass Spectrometry and Nuclear Magnetic Resonance. Using a purpose-built computational tool for analysing lipid metabolism as a network, we characterised the number, type and abundance of lipid variables in and between tissues (Lipid Traffic Analysis), finding a variety of alterations associated with paternal diet. These elucidate a mechanism for the defective physiological behaviour of systems at risk of cardio-metabolic disease.

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Section: Resultsmentioning

confidence: 99%

Section: Di-ms Data Processingmentioning

confidence: 99%

Revealing grand-paternal programming of lipid metabolism using a novel computational tool

Furse

Watkins

Chiarugi

et al. 2020

Preprint

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“…Meta-analyses indicate that this can have a considerable impact on the relative and absolute abundance of lipids that is recorded and published (Furse et al 2015). A similar effect has been observed in lipid isolation procedures, leading to the development of new methods (Furse et al 2017). This hints that there are features of studies that cannot be altered if they are to be comparable, and thorough validation of methods used is necessary to minimise the risk of this.…”

Section: Future Perspectivesmentioning

confidence: 88%

“…A similar effect has been observed in lipid isolation procedures, leading to the development of new methods (Furse et al . ). This hints that there are features of studies that cannot be altered if they are to be comparable, and thorough validation of methods used is necessary to minimise the risk of this.…”

Section: Future Perspectivesmentioning

confidence: 97%

The validation of biomarkers of metabolic efficacy in infant nutrition

2018

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Breastfeeding is regarded as the ideal way to nourish infants. However, feeding with formula milk is also common in much of the West. Despite this, the function of the molecular components of breast and formula milks are not fully understood, less still the relationship between the composition of the milk and the infant's metabolism and how this influences the infant's development. The Biotechnology and Biological Sciences Research Council‐funded project ‘The validation of biomarkers of metabolic efficacy in infant nutrition’ aims to identify lipid biomarkers that can be used to study the effect of diet on growth and development of infants. In this work, we have been able to validate these markers. Here, we present an approach to biomarker discovery that has new depth and will inform research questions about how metabolism is governed, and which species can be used to identify situations where metabolism is becoming defective.

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“…The binding of α -Syn may be further promoted by its potential for desolvating exposed hydrophobic sites between small headgroups the bilayer, alleviating the entropic cost associated with this and at the same time reducing curvature elastic stress 39,65 . For α -Syn binding to PE-containing lipids these exposed sites may be more important in the absence of strong electrostatic interactions.…”

Section: Introductionmentioning

confidence: 99%

Cholesterol is a strong promotor of an α-Synuclein membrane binding mode that accelerates oligomerization

Jakubec

Bariås

Furse

et al. 2019

Preprint

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Dysregulation of the biosynthesis of cholesterol and other lipids has been implicated in neurological diseases, including Parkinson's disease, where the misfolding of membraneassociated α-Synuclein is a key molecular event. Recent research also suggests that α-Synuclein aggregation is influenced by the lipid environment. The exact molecular mechanisms responsible for cholesterol’s effect on α-Synuclein binding to lipids and how this binding may affect α-Synuclein oligomerization and fibrillation remain elusive, as does the relative importance of cholesterol versus other lipid factors. We probed the interactions and fibrillation behaviour of α-Synuclein using SMA nanodiscs, containing zwitterionic and anionic lipid model systems with and without cholesterol. SPR and ThT fluorescence assays were then employed to monitor α-Synuclein binding, as well as fibrillation in the absence and presence of membrane models. 1H-15N correlated NMR was used to monitor the fold of α-Synuclein in response to nanodisc binding, and we determined individual residue apparent affinities for the nanodisc-contained bilayers. Cholesterol inhibited α-Synuclein interaction with lipid bilayers. We also find that cholesterol significantly promotes α-Synuclein fibrillation, with a more than 20-fold reduction of lag-times before fibrillation onset. When α-Synuclein-bilayer interactions were analysed for individual residues by solution-state NMR, we observed two different effects of cholesterol. In nanodiscs made of DOPC, cholesterol modulated the NAC part of α-Synuclein, leading to stronger interaction of this region with the lipid bilayer. In contrast, in the nanodiscs comprising DOPC, DOPE and DOPG, the NAC part was mostly unaffected by cholesterol, while the binding of the N-terminal and the C-terminal were both inhibited.

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Evidence that Listeria innocua modulates its membrane’s stored curvature elastic stress, but not fluidity, through the cell cycle

Cited by 32 publications

References 79 publications

Revealing grand-paternal programming of lipid metabolism using a novel computational tool

Revealing grand-paternal programming of lipid metabolism using a novel computational tool

The validation of biomarkers of metabolic efficacy in infant nutrition

Cholesterol is a strong promotor of an α-Synuclein membrane binding mode that accelerates oligomerization

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