Evidence that L-(+)-lactate dehydrogenase deficiency is lethal in Streptococcus mutans

Hillman, Jeffrey D.; Chen, A.; Duncan, Marilyn J.; Lee, Seok‐Woo

doi:10.1128/iai.62.1.60-64.1994

Cited by 29 publications

(17 citation statements)

References 11 publications

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“…are: Fru, fructose; Gal, galactose; LDH, lactate dehydrogenase; PFL, pyruvate-formiate lyase; Pi, inorganic orthophosphate; PK, pyruvate kinase. et al, 1987;Hillman et al, 1994;de Soet et al, 2000;Takahashi-Abbe et al, 2003). These extracellular compounds being the responsible of acidic activity ascribed to S. mutans (Takahashi et al, 1991;de Soet et al, 2000;Takahashi and Nyvad, 2011).…”

Section: Discussionmentioning

confidence: 99%

The ADP‐glucose pyrophosphorylase from Streptococcus mutans provides evidence for the regulation of polysaccharide biosynthesis in Firmicutes

Diez

Demonte

Guerrero

et al. 2013

Molecular Microbiology

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Streptococcus mutans is the leading cause of dental caries worldwide. The bacterium accumulates a glycogen-like internal polysaccharide, which mainly contributes to its carionegic capacity. S.mutans has two genes (glgC and glgD) respectively encoding putative ADP-glucose pyrophosphorylases (ADP-Glc PPase), a key enzyme for glycogen synthesis in most bacteria. Herein, we report the molecular cloning and recombinant expression of both genes (separately or together) followed by the characterization of the respective enzymes. When expressed individually GlgC had ADP-Glc PPase activity, whereas GlgD was inactive. Interestingly, the coexpressed GlgC/GlgD protein was one order of magnitude more active than GlgC alone. Kinetic characterization of GlgC and GlgC/GlgD pointed out remarkable differences between them. Fructose-1,6-bis-phosphate activated GlgC by twofold, but had no effect on GlgC/GlgD. Conversely, phospho-enol-pyruvate and inorganic salts inhibited GlgC/GlgD without affecting GlgC. However, in the presence of fructose-1,6-bis-phosphate GlgC acquired a GlgC/GlgD-like behaviour, becoming sensitive to the stated inhibitors. Results indicate that S. mutans ADP-Glc PPase is an allosteric regulatory enzyme exhibiting sensitivity to modulation by key intermediates of carbohydrates metabolism in the cell. The particular regulatory properties of the S.mutans enzyme agree with phylogenetic analysis, where GlgC and GlgD proteins found in other Firmicutes arrange in distinctive clusters.

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Section: Discussionmentioning

confidence: 99%

The ADP‐glucose pyrophosphorylase from Streptococcus mutans provides evidence for the regulation of polysaccharide biosynthesis in Firmicutes

Diez

Demonte

Guerrero

et al. 2013

Molecular Microbiology

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“…Through BLAST, we found that HM9 ( l ‐lactate dehydrogenase) is highly conserved among different bacterial species. Research has mainly focused on the functions of this enzyme (Hillman et al., 1994; Wyckoff et al., 1997), and it was reported that l ‐lactate dehydrogenase was identified as one of the predominant immunogenic proteins in pigs naturally or experimentally infected with Mycoplasma hyopneumoniae (Frey et al., 1994).…”

Section: Discussionmentioning

confidence: 99%

Immunoproteomic Assay of Membrane‐associated Proteins ofStreptococcus suisType 2 China Vaccine Strain HA9801

Zhang

Chen

2007

Zoonoses and Public Health

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Immunoproteomic approaches were undertaken to study the immunogenicity of the membrane-associated proteins of the Streptococcus suis type 2 (SS2) China vaccine strain HA9801. The membrane-associated proteins were enriched using the Triton X-114 extraction protocol and were analysed by two-dimensional gel electrophoresis (2-DE) and subsequent immunoblotting using the hyperimmune serum of SS2-HA9801-immunized specific pathogen free (SPF) minipigs. A total of 11 proteins were recognized, and the corresponding spots on a duplicate gel were excised and identified by MALDI-TOF MS.

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“…Regarding its pathogenicity, lactate dehydrogenase (LDH) deficiency can be used as the approach for reducing acidogenicity in the construction of the BCS3-L1 strain. 70 Cloning the structural gene encoding the S. mutans LDH provided the basis for producing LDH-deficient clones, 71,72 suggesting that LDH-deficiency was a lethal mutation in most S. mutans strains. However, at high sugar concentrations, the levels of activity of these enzymes are ap-parently insufficient to compensate for the absence of LDH.…”

Section: Replacement Therapymentioning

confidence: 99%

Present and Future Approaches for the Control of Caries

Anusavice

2005

Journal of Dental Education

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This article summarizes current and potential future approaches for the management of caries. Current surveys suggest that traditional "drill, fill, and bill" dentistry is still widely practiced in the United States in spite of considerable evidence that supports a minimally invasive treatment approach. Because there is a wide variability in treatment decisions on when and how to prevent new lesions, on how to arrest the progression of existing lesions, and on when and how to place initial and replacement restorations, the findings from some studies differ significantly from the results of other studies. While fluoride treatments are known to prevent a percentage of new lesions, they do not have the ability to prevent all caries lesions. Modern management of caries entails treating patients according to risk and monitoring early lesions in tooth surfaces that are not cavitated. Although we know that the dmfs score for children is a powerful predictor of caries increment in permanent teeth of these children a few years later, this score is rarely used in private practice as a measure of risk or as a measure of treatment success. Although these modern methods for caries management offer great promise for controlling the disease, they may take decades to apply in a standardized way so that the variability in treatment is reduced. However, during the next two decades, an alternative approach to caries prevention such as replacement therapy and a caries vaccine may become available as a more consistent method of controlling this disease. Dr. Anusavice is Associate

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Evidence that L-(+)-lactate dehydrogenase deficiency is lethal in Streptococcus mutans

Cited by 29 publications

References 11 publications

The ADP‐glucose pyrophosphorylase from Streptococcus mutans provides evidence for the regulation of polysaccharide biosynthesis in Firmicutes

The ADP‐glucose pyrophosphorylase from Streptococcus mutans provides evidence for the regulation of polysaccharide biosynthesis in Firmicutes

Immunoproteomic Assay of Membrane‐associated Proteins ofStreptococcus suisType 2 China Vaccine Strain HA9801

Present and Future Approaches for the Control of Caries

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