Evidence that Human Cytomegalovirus Assembly Protein Shares Antigenic Sites with an Uninfected Cell Membrane Protein

Landini, Maria Paola; Lazzarotto, Tiziana; Percivalle, Elena; Ripalti, Alessandro; Gerna, Giuseppe

doi:10.1099/0022-1317-72-12-3009

Cited by 11 publications

(3 citation statements)

References 16 publications

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“…CMV infection might result in auto‐reactive B‐lymphocyte clones. Cross‐reactivity between viral epitopes and erythrocytic antigens is also possible [4].…”

mentioning

confidence: 99%

Rituximab therapy for cytomegalovirus-associated auto-immune hemolytic anemia in a liver-transplant recipient

et al. 2008

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“…CMV infection might result in auto‐reactive B‐lymphocyte clones. Cross‐reactivity between viral epitopes and erythrocytic antigens is also possible [4].…”

mentioning

confidence: 99%

Rituximab therapy for cytomegalovirus-associated auto-immune hemolytic anemia in a liver-transplant recipient

et al. 2008

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“…However, we cannot exclude the possibility that the reactivity to pp150 is not due to ppUL32 but, rather, to the other structural protein with a molecular mass of 150 kDa (pUL86) which is conserved among herpesviruses. In addition, a nonspecific reaction to pp38 could be due to some cross-reactivity of this protein with a cell membrane protein of 60 kDa (12). Therefore, some serum samples that are IgM reactive only to 150-or 38-kDa proteins on the viral WB may not be due to HCMV-specific IgM.…”

mentioning

confidence: 99%

Detection of serum immunoglobulin M to human cytomegalovirus by western blotting correlates better with virological data than detection by conventional enzyme immunoassay

Lazzarotto

Maine

Monte

et al. 1996

Clin Diagn Lab Immunol

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Western blotting (immunoblotting) with proteins separated from purified human cytomegalovirus (HCMV) particles (viral WB) has repeatedly been shown to be a reliable and sensitive method for detecting HCMVspecific immunoglobulin M (IgM). The aim of the present work was to determine whether IgM detected by viral WB correlates with virological diagnosis better than conventional enzyme immunoassay (conv-EIA). The presence of an active HCMV infection was documented on the basis of isolation of virus from urine and/or saliva and on the basis of antigenemia and/or PCR with polymorphonuclear leukocytes for immunocompetent and immunocompromised subjects, respectively. The agreement observed between IgM detected by viral WB and the results obtained by virological detection of HCMV was significantly higher (88.7%) than the agreement of IgM detected by conv-EIA and virological results (67.5%).

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“…Although CMV has sometimes been associated with hemolytic anemia, particularly in immunocompromised patients, the reason for this is not completely clear. One documented mechanism of CMV pathogenicity is molecular mimicry between antigens on erythrocytes and CMV‐infected cells, for example mp60 on RBC and vp38 on CMV‐infected cells, resulting in cross‐reactions . To rule out this possibility, we determined whether membrane‐associated IgG showed measurable CMV antibody activity.…”

Section: Discussionmentioning

confidence: 99%

Case of cytomegalovirus‐associated direct anti‐globulin test‐negative autoimmune hemolytic anemia

Kaneko

Sato

Sasaki

et al. 2013

Pediatrics International

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A 1-year-old boy developed autoimmune hemolytic anemia after a negative direct anti-globulin test. The concentration of erythrocyte membrane-associated immunoglobulin G, determined using an immunoradiometric assay, correlated with disease activity. He was positive for cytomegalovirus (CMV) both serologically and by quantitative real-time polymerase chain reaction, indicating that his autoimmune hemolytic anemia was directly caused by CMV infection. Since anti-CMV immunoglobulin G was not absorbed by the patient's erythrocytes, cross-reaction between erythrocyte antigens and CMV was not likely a causative factor for hemolysis.

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Evidence that Human Cytomegalovirus Assembly Protein Shares Antigenic Sites with an Uninfected Cell Membrane Protein

Cited by 11 publications

References 16 publications

Rituximab therapy for cytomegalovirus-associated auto-immune hemolytic anemia in a liver-transplant recipient

Rituximab therapy for cytomegalovirus-associated auto-immune hemolytic anemia in a liver-transplant recipient

Detection of serum immunoglobulin M to human cytomegalovirus by western blotting correlates better with virological data than detection by conventional enzyme immunoassay

Case of cytomegalovirus‐associated direct anti‐globulin test‐negative autoimmune hemolytic anemia

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