Evidence that cyclosporin A administration induces the formation of new cementum‐like islets inside the gingival connective tissue

Ayanoglou, Charles M.

doi:10.1111/j.1600-0765.1998.tb02307.x

Cited by 8 publications

(1 citation statement)

References 12 publications

(11 reference statements)

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“…Thus, one possibility is that the PTPLA-CAP may regulate the phosphatase activity of PTPLA. Interestingly, cyclosporine A, which inhibits calcineurin, another phosphatase, also induces cementum formation and mineralization (Ayanouglou, 1998; Arzate et al ., 2005). If this is the case, the outcome of PTPLA-CAP action is likely to be different in other tissues, which are not mineralized, where it is expressed.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Protein-Tyrosine Phosphatase-like Member-a Variant from Cementum

Hoyos

Hoz-Rodríguez²,

Arzate

et al. 2011

J Dent Res

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A supplemental appendix to this article is published electronically only at http://jdr.sagepub.com/supplemental. AbstrAct Cementum has been shown to contain unique polypeptides that participate in cell recruitment and differentiation during cementum formation. We report the isolation of a cDNA variant for protein-tyrosine phosphatase-like (proline instead of catalytic arginine) member-a (PTPLA) from cementum. A cementifying fibroma-derived λ-ZAP expression library was screened by panning with a monoclonal antibody to cementum attachment protein (CAP), and 1435 bp cDNA (gb AC093525.3) was isolated. This cDNA encodes a 140-amino-acid polypeptide, and its N-terminal 125 amino acids are identical to those of PTPLA. This isoform, designated as PTPLA-CAP, results from a read-through of the PTPLA exon 2 splice donor site, truncating after the second putative transmembrane domain. It contains 15 amino acids encoded within the intron between PTPLA exons 2 and 3, which replace the active site for PTPLA phosphatase activity. The recombinant protein, rhPTPLA-CAP, has Mr 19 kDa and cross-reacts with anti-CAP antibody. Anti-rhPTPLA-CAP antibody immunostained cementum cells, cementum, heart, and liver. Quantitative RT-PCR showed that PTPLA was expressed in all periodontal cells; however, PTPLA-CAP expression was limited to cementum cells. The rhPTPLA-CAP promoted gingival fibroblast attachment. We conclude that PTPLA-CAP is a splice variant of PTPLA, and that, in the periodontium, cementum and cementum cells express this variant.

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Section: Discussionmentioning

confidence: 99%

Isolation of Protein-Tyrosine Phosphatase-like Member-a Variant from Cementum

Hoyos

Hoz-Rodríguez²,

Arzate

et al. 2011

J Dent Res

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Oral manifestations associated with inherited hyperhomocysteinemia: A first case description

Husseini

Nehmé

Senni

et al. 2022

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

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Phagocytosis of new cementum‐like islets formed inside the gingival connective tissue in cyclosporin‐A treated rats

Ayanoglou

1999

J of Periodontal Research

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Three control and 3 experimental rats were administered vehicle or cyclosporin-A solutions. Animals were anaesthetized, tissues fixed and jaws processed for Epon inclusion. Histological examination of serially cut areas revealed the presence inside the gingival connective tissue of new cementum-like islets (NCLIs) associated to or engulfed by voluminous multinucleated cells (MCs). These complexes were located adjacent to blood vessels, at 250-350 microns from the root surface. Histomorphometric study indicated that the volume of the NCLIs varied from 3900 to 72,900 microns 3 and that of the MCs from 822 to 56,190 microns 3. The latter bore up to 14 nuclear profiles. Comparative evaluation of the NCLI-MC associations to other complexes "multinucleated cell(s)-resorbed material" seems to indicate that the phagocytosis of the NCLIs is dictated by their ectopic location, rather than their nature (new cementum-like structures). Therefore the MCs should be considered as foreign body giant multinucleated cells rather than cementoclasts.

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Evidence that cyclosporin A administration induces the formation of new cementum‐like islets inside the gingival connective tissue

Cited by 8 publications

References 12 publications

Isolation of Protein-Tyrosine Phosphatase-like Member-a Variant from Cementum

Isolation of Protein-Tyrosine Phosphatase-like Member-a Variant from Cementum

Oral manifestations associated with inherited hyperhomocysteinemia: A first case description

Phagocytosis of new cementum‐like islets formed inside the gingival connective tissue in cyclosporin‐A treated rats

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