Evidence that an Arg-Gly-Asp Adhesion Sequence Plays a Role in Mammalian Fertilization

Bronson, Richard; Fusi, Francesco

doi:10.1095/biolreprod43.6.1019

Cited by 139 publications

(73 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…According to Bronson and Fusi (1990), peptides containing the Arg-Gly-Asp (RGD) adhesion sequence play a role in spermatozoon-oolemma adhesion. Since C3b contains an RGD sequence (Wright et al, 1987) and since C3 binds to acrosome-reacted spermatozoa (Anderson et al, 1990), it could be that endometrial C3 is involved in spermatozoon-egg interaction.…”

Section: Discussionmentioning

confidence: 99%

Immunology: Investigations on the cell type responsible for the endometrial secretion of complement component 3 (C3)

et al. 1994

View full text Add to dashboard Cite

It has been shown that rat and human endometria have the capacity to produce complement component 3 (C3). In rats, endometrial C3 is an oestrogen-dependent protein produced and secreted by glandular cells. The cell responsible for the synthesis and secretion of human endometrial C3 has not been clearly defined. Our study was aimed at answering this question. Samples of endometrium obtained from hysterectomies were either immunostained for C3 or digested with collagenase; then the stromal and glandular cells were separated and immunopurified (or not) with an antibody to CD45 coupled to magnetic beads to eliminate the endometrial lymphomyeloid cells. Cells were cultured for 2 weeks and C3 measured in the medium by an in-house radioimmunoassay. Glandular as well as stromal cells stained positively for C3 and released C3 in vitro. The release of C3 from both cell types could be inhibited by cycloheximide. Epithelial cells produced significantly more C3 than stromal cells, and endometrial C3 production was higher for both cell types when these were obtained from secretory as compared to proliferative endometria. Lymphomyeloid cells were possibly a source of C3 since after immunoadsorption of these cells, the remaining stromal or glandular cells produced significantly less C3. We conclude that endometrial stromal, glandular and lymphomyeloid cells all produce C3.

show abstract

Section: Discussionmentioning

confidence: 99%

Immunology: Investigations on the cell type responsible for the endometrial secretion of complement component 3 (C3)

et al. 1994

View full text Add to dashboard Cite

show abstract

“…Alternatively, as the YIGSR peptide was found to inhibit angiogenesis (28), the anti-YIGSR antibodies also might have inhibited placental vascularization. In the case of the infertile RGD-immunized monkey, the anti-RGD antibodies could have interfered with sperm-egg attachment, as RGD peptides were found to prevent human sperm-hamster egg attachment in vitro (29). However, similar studies with isolated antibodies from the RGD-immunized monkey were inconclusive (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Reproduction and sera embryotoxicity after immunization of monkeys with the laminin peptides YIGSR, RGD, and IKVAV.

Chambers¹,

Klein²,

Conrad³

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Monkeys with excellent reproductive histories were immunized with the laminin peptides YIGSR, RGD, IKVAV, and YD, a control sequence with no known biological function. Sera from the YIGSR-immunized monkey became toxic, causing neural tube defects in whole rat embryo cultures, and this monkey experienced fetal loss after immunization. Sera from the RGD-immunized monkey also became embryotoxic in culture after immunization, but this monkey appeared to become infertile as she failed to initiate a pregnancy for at least 2 years after immunization. In contrast, embryos cultured on sera from the IKVAV-or YD-immunized monkeys were predominantly normal and both monkeys completed successful pregnancies. Antibody levels to the respective peptides or to laminin were not predictive of embryotoxicity, but antibody binding to homogenized yolk sacs as well as to yolk sacs of cultured embryos was associated with sera embryotoxicity and reproductive outcomes in vivo. These observations suggested that the laminin sequences YIGSR and RGD may play a role in immune-mediated reproductive failure by reacting directly with embryonic tissue and could provide a basis for identifying individuals at risk for both spontaneous abortion and infertility.Immune dysfunction has been implicated in recurrent pregnancy loss (1). For example, isolated T cells from recurrent aborters were found to secrete embryotoxic cytokines in response to trophoblast cells (2), while a direct role of autoantibodies in fetal loss has been implicated for women with autoimmune diseases, such as systemic lupus erythematosus (3) and herpes gestationis (4). Anti-phospholipid autoantibodies particularly have been associated with spontaneous abortions (5), but their presence alone could not predict poor pregnancy outcomes (6). Therefore, there has been a need to identify specific autoantibodies responsible for reproductive failure and to establish their mechanisms of pathogenicity (7).Anti-laminin autoantibodies were detected in the sera of some monkeys with histories of reproductive failure, and sera from these monkeys caused neural tube defects in cultures of whole rat embryos (8). When monkeys with excellent reproductive histories were immunized with intact murine laminin, their sera became embryotoxic, again causing neural tube defects in rat embryo cultures, and these monkeys subsequently failed to reproduce (9). When pregnant mice were injected with heterologous anti-laminin antibodies, they spontaneously aborted (10). However, sera from lamininimmunized rats were not consistently embryotoxic and sera anti-laminin antibody levels were not predictive of embryotoxicity (11). These toxic and nontoxic sera were found to recognize different epitopes on Western blots of enzymedigested laminin, suggesting that antibodies to only certainThe publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact. laminin epitopes were r...

show abstract

“…A suspected role in implantation has been heightened by the observation that the Arg-Gly-Asp (RGD) adhesion sequence present on many ECM molecules may be involved in the process of fertilization (Bronson and Fusi, 1990) and in trophoblast attachment and outgrowth in vitro (Kao et al, 1988). Aplin and coworkers (1988) examined a number of ECM species throughout the menstrual cycle and in early pregnancy in humans.…”

Section: Secretory Proteins Of the Luteal Endometriummentioning

confidence: 99%

Immunohistochemical markers of uterine receptivity in the human endometrium

Ilesanmi

Hawkins

Lessey

1993

Microscopy Res & Technique

View full text Add to dashboard Cite

The factors responsible for the initial interaction between maternal and fetal epithelium leading to the establishment of pregnancy remain poorly understood. Temporal and spatial expression of specific endometrial peptides in response to ovarian steroids is thought to contribute to the development of a period of uterine receptivity, whereby the endometrium becomes hospitable to the implanting blastocyst. The failure to establish receptivity may account for a significant percentage of the cases of infertility in the female, especially affecting women with luteal phase deficiency, leiomyomata uteri, endometriosis, habitual abortion, and unexplained infertility. In addition, despite increasing global experience with advanced reproductive technologies, the majority of In Vitro Fertilization (IVF) attempts remain unsuccessful, most likely on the basis of implantation failure. In this article, we review the concepts involved in the study of uterine receptivity in the human, highlight potential immunohistochemical (IHC) markers that have recently been discovered, and discuss how IHC assessment of the endometrium is a potentially valuable method for the evaluation of the receptive endometrial state. Using this approach we have examined several new potential markers of uterine receptivity. Endometrial progesterone receptors and one of the integrin cell adhesion molecules appear to undergo changes in expression around the time of implantation, and may be sensitive indicators of the receptive state. Further, these markers are delayed in women with infertility and luteal phase deficiency. These studies illustrate the utility of IHC diagnosis for the evaluation of endometrial function.

show abstract

Evidence that an Arg-Gly-Asp Adhesion Sequence Plays a Role in Mammalian Fertilization

Cited by 139 publications

References 20 publications

Immunology: Investigations on the cell type responsible for the endometrial secretion of complement component 3 (C3)

Immunology: Investigations on the cell type responsible for the endometrial secretion of complement component 3 (C3)

Reproduction and sera embryotoxicity after immunization of monkeys with the laminin peptides YIGSR, RGD, and IKVAV.

Immunohistochemical markers of uterine receptivity in the human endometrium

Contact Info

Product

Resources

About