1982
DOI: 10.1016/0009-8981(82)90107-3
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Evidence that alkaline phosphatase from human neutrophils is the same gene product as the liver/kidney/bone isoenzyme

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Cited by 45 publications
(18 citation statements)
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“…The rate of solubilization of enzymes is higher in cytoplasm than in membrane prep aration [22]. It is difficult to assess the com plete recovery of membrane-linked compo nents with Triton X-100 alone; the yield is about 70-80% [14].…”
Section: Isoenzyme Types Found In Pmnmentioning
confidence: 99%
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“…The rate of solubilization of enzymes is higher in cytoplasm than in membrane prep aration [22]. It is difficult to assess the com plete recovery of membrane-linked compo nents with Triton X-100 alone; the yield is about 70-80% [14].…”
Section: Isoenzyme Types Found In Pmnmentioning
confidence: 99%
“…Recently, Gainer and Stinson [14] have analyzed the inhibition pattern of normal NAP in man. They postulated that NAP is identical to liver AP.…”
Section: Isoenzyme Types Found In Pmnmentioning
confidence: 99%
See 1 more Smart Citation
“…The ALP from tissues which express the tis sue-nonspecific enzyme (e.g. liver, bone, kid ney) all show indistinguishable patterns of inhibition with the inhibitors homoarginine, phenylglycylglycine, and phenylalanine [22,23], The inhibitor profile for the ALP from the lymphoma cells was also indistinguishable from the tissue-nonspecific isoenzyme (ta ble 2), but different from the isoenzymes of intestine and placenta.…”
Section: Discussionmentioning
confidence: 89%
“…Informed written consent was obtained from each individual. [ 10] procedure, kept in Krebs-Ringer buffer, were lysed by sonication (three bursts at 50 W for 30 s) and NAP was solubilized with 2% Triton X-100 and 20% n-butanol. After centrifugation at 6,000# for 15 min, the alcoholic phase was discarded and the aqueous layer recovered.…”
Section: Subjectsmentioning
confidence: 99%