Evidence that a human soluble beta-galactoside-binding lectin is encoded by a family of genes.

Abstract

Two cDNA clones were isolated by immunoscreening a human hepatoma cDNA library with an antiserum that bound specifically to a human soluble (-galactoside-binding lectin with Mr of t14,000. The deduced amino acid sequences of the inserts of these two clones show considerable homology with each other, the sequence of chicken skin P-galactoside-binding lectin, and eight peptides derived from purified human lung lectin of Mr =14,000. However, the sequence differences between the two hepatoma clones as well as among each clone and the lung peptides suggest that at least three variants of the gene encoding this lectin are expressed in human tissue.

Selective expression of an endogenous lactose-binding lectin gene in subsets of central and peripheral neurons

“…Expression of RL-14.5 mRNA persists in essentially all DRG neurons at later developmental stages and is maintained (Wilson et al, 1989); human placenta (Hirabayashi and Kasai, 1988) chick (Ohyama et al, 1986), and eel (Paroutaud et al, 1987). b, Alignment of the sequence of RL-14.5 with homologous proteins of higher or undetermined molecular weight including human Hepl (Gitt and Barondes, 1986); the carboxy-terminal region (amino acids 127-262) of mouse CBP-35 (Jia and Wang, 1988) and of the carboxyterminal region (amino acids 128-263) of the rat IgE binding protein (Albrandt et al, 1987). in adult sensory neurons (Fig. 6).…”

“…To address this question and to provide further information on the structure and expression of the neural 14 kDa lectin, we isolated cDNA clones encoding the 14 kDa lectin present in the rat nervous system. Adult rat brain XGTlO and XGT 11 cDNA libraries were screened with a cDNA designated Hep-2 that was isolated from a HEP-G2 hepatoma cDNA library (Gitt and Barondes, 1986). The Hep-2 cDNA is similar or identical to the sequence of the cDNA encoding a mouse 14 kDa lectin (Wilson et al, 1989).…”

“…and Methods isolation and sequence analysis of neural lectin cDNAs.Adult rat brain cDNA libraries kindly provided by Jurgen Brosius or obtained from Clontech were screened by the filter hybridization method (Maniatis et al, 1982) with a 32P-labeled (Weinstock et al, 1978) cDNA fragment (Hep-2) encoding a protein recognized by antibodies against RL-14.5 (Gitt and Barondes, 1986). In addition, a XGTll cDNA expression library prepared from RNA of El5 spinal cord (S. Temple and T. M. Jessell, unpublished observations) was screened with antibodies against RL-14.5 (Cerra et al, 1984) using published procedures (Young and Davis, 1983;Snyder and Davis, 1985).…”

A scanning electrochemical microscopy approach for bio assays on microtissue arrays

“…An extension of this approach is towards single cells that are involved in aerobic respiration, which in contrast to plant systems actively consume oxygen for energy production. Therefore, live bacterial or mammalian cells can be imaged by observing lowered oxygen concentrations in their vicinity, when compared to bulk solution [28][29][30]. Similarly, more complex models such as microtissues and embryos have also been assessed for their oxygen consumption [31,32].…”

“…Increased expression of ezrin (EZRI), moesin (MOES), galectin-1 (LEG1) [28], vinculin (VINC) [29] indicate cell-cell adhesion, focal adhesion and membrane to membrane docking which are essential for tissue formation. Several proteins-14-3-3 protein gamma (1433G), fascin (FSCN1) [30,31], prolifin-1 (PROF1) [32], src substrate cortactin (SRC8) [33] show cytoskeletal reorganisation which is expected as shown in the confocal image in Fig.…”