Evidence on inhibition of Listeria monocytogenes by divercin V41 action

Abstract

Aims: The aim of this study was to investigate the role of divercin V41 in inhibition and prevention of Listeria monocytogenes. Methods and Results: Carnobacterium divergens V41 deficient in bacteriocin production was isolated and characterized by enzyme‐liked immunosorbent assay, multiplex polymerase chain reaction and bacteriocin diffusion test. Carnobacterium divergens V41 (divercin+) and Carnobacterium divergens V41C9 (divercin−) were grown in the presence of L. monocytogenes in smoked salmon model medium. Carnobacterium divergens V41, but not C. divergens V41C9, was able to inhibit growth of L. monocytogenes. The results indicate that inhibition of L. monocytogenes in the presence of C. divergens V41 is because of the production of divercin V41 and not to a nutritional advantage. Conclusions: Carnobacterium divergens V41 may be a promising agent in food safety. Significance and Impact of the Study: The study demonstrates a potential use of a bacteriocin producing lactic acid bacteria in the area food protection.

Generation and Utilization of Polyclonal Antibodies to a Synthetic C-Terminal Amino Acid Fragment of Divercin V41, a Class IIa Bacteriocin

“…Prior to ELISA, the presence or absence of bacteriocin activity in each supernatant was determined by the ADT using Listeria innocua F as the indicator organism. As expected, the anti-DvnCt-KLH antibodies showed a high reactivity with the supernatant from C. divergens V41 cultures, but no cross-reactivity was detected with supernatants from cultures of C. divergens V41C9 (divV41 Ϫ ), a mutant strain deficient in divercinV41 production (35). As noted previously (25), the use of mutant strains devoid of bacteriocin synthesis is useful in evaluating the specificity and immunoreactivity of polyclonal antipeptide antibodies, since the only difference between the wild-type and mutant strains is the presence or absence of bacteriocin activity in their supernatants.…”

“…The cleavage of divercin V41 by endoproteinase Asp-N releases an inactive hydrophilic Nterminal fragment and a hydrophobic C-terminal fragment active against Listeria monocytogenes (4). Recently, we demonstrated the role of divercin V41 in inhibition of Listeria monocytogenes in smoked salmon (35). This paper describes the generation of polyclonal antibodies against a chemically synthesized C-terminal fragment of divercin V41.…”

Insights into Structure-Activity Relationships in the C-Terminal Region of Divercin V41, a Class IIa Bacteriocin with High-Level Antilisterial Activity

“…The broad spectrum of activity of DvnV41 against food-borne pathogenic bacteria, such as Listeria monocytogenes, in vitro and in vivo (43,46) has attracted great interest and led to its heterologous production in Escherichia coli (43,59) to enhance the peptide yield and simplify the purification procedure (45,60). DvnV41 is synthesized as a prebacteriocin consisting of 66 amino acids.…”

Article

“…Récemment, l'utilisation d'un mutant défi-cient dans la production de divercine V41 a permis de démontrer le rôle déterminant de la production de divercine dans l'activité inhibitrice de L. monocytogenes dans le saumon fumé par Cb. divergens V41 [41].…”