Evidence of two XX/XY sex-determining loci in the Stirling stock of Nile tilapia (Oreochromis niloticus)

Taslima, Khanam; Khan, Mohd Golam Quader; McAndrew, Brendan; Penman, David J.

doi:10.1016/j.aquaculture.2020.735995

Cited by 13 publications

(24 citation statements)

References 55 publications

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“…In the present study, the combination of these two, three, and four markers (Amh, SCAR4, SCAR5, and Oni3161) markers showed an increasing association with phenotypic sex characteristics of the red tilapia with an accuracy of 63.0-72.0%. This result is in agreement with previous studies, which demonstrated that the combinations of sex-linked markers on LG1, LG20, and LG23 were associated with QTL regions for sex determination in Nile tilapia populations (Palaiokostas et al, 2015;Taslima et al, 2021). Palaiokostas et al (2015) reported that allelic combinations of SNP markers on LG1 (rs397507167) and LG20 (Oni3161) were highest associated with phenotypic sex in offspring of the Egyptian Nile tilapia population.…”

Section: Discussionsupporting

confidence: 93%

“…Moreover, the SCAR4 and SCAR5 markers had also been mapped on LG23 of Nile tilapia (Sun et al, 2014). Additionally, previous studies have demonstrated that the Oni3161 marker is significantly associated with sex-determining QTL regions of the Egyptian Nile tilapia population (Palaiokostas et al, 2015;Taslima et al, 2021). The Oni3161 marker had been localized on LG20 of Nile tilapia (Palaiokostas et al, 2015).…”

Section: Discussionmentioning

confidence: 95%

“…Moreover, it has been reported that the Amh gene had the highest overexpression in male compared to female embryos of Nile tilapia at 3 to 7 days postfertilization (Eshel et al, 2012). The Amh gene is a member of the transforming growth factor beta (TGF-β) family and is secreted by Sertoli cells and is responsible for the regression of Müllerian duct during male fetal development in mammals, birds, and reptiles (Taslima et al, 2021). Moreover, sequencing analysis of the Amh gene in Nile tilapia revealed a male-specific duplication, denoted Amhy, differing from the sequence of Amh by a 233 bp deletion on exon VII.…”

Section: Discussionmentioning

confidence: 99%

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Association of genetic markers with sex determination in Thai red tilapia

Naraballobh

Pothakam²,

Norseeda

et al. 2021

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The objectives of this study were to verify the polymorphism on sex-linked marker loci and to assess their associations with phenotypic sex characteristics in red tilapia. Four sex-linked genetic markers of Amh, SCAR4, SCAR5, and Oni3161 were genotyped in the Thai red tilapia population. The Amh marker was significantly associated with the phenotypic sex of red tilapia with an accuracy of 46.2%. No significant association of SCAR4, SCAR5, and Oni3161 marker polymorphisms with phenotypic sex characteristics was observed in this study. However, the combinations of these two, three, and four markers were increasingly associated with phenotypic sex characteristics for red tilapia with an accuracy of 62.8, 68.4, and 72.4%, respectively. These results indicate that these combined genetic markers were associated with the phenotypic sex of red tilapias. These findings confirmed the importance of these genetic markers as candidate markers for sex determination in the Thai red tilapia population.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

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Association of genetic markers with sex determination in Thai red tilapia

Naraballobh

Pothakam²,

Norseeda

et al. 2021

VIS

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“…Research on sex determination in tilapia has important economic implications because of the different growth rates between sexes, and therefore research on the molecular basis of sex determination in tilapia has been conducted for decades. Oreochromis niloticus has one of the best-documented sex determination systems, with major sex determining regions characterized on LG1, LG20 and LG23 ( Lee et al, 2003 ; Li et al, 2015 ; Palaiokostas et al, 2015 ; Taslima et al, 2021 ). Recently, sex determining systems have been characterized in several other tilapias, including S .…”

Section: Discussionmentioning

confidence: 99%

A Chromosome-Level Genome Assembly of Mozambique Tilapia (Oreochromis mossambicus) Reveals the Structure of Sex Determining Regions

et al. 2021

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The Mozambique tilapia (Oreochromis mossambicus) is a fascinating taxon for evolutionary and ecological research. It is an important food fish and one of the most widely distributed tilapias. Because males grow faster than females, genetically male tilapia are preferred in aquaculture. However, studies of sex determination and sex control in O. mossambicus have been hindered by the limited characterization of the genome. To address this gap, we assembled a high-quality genome of O. mossambicus, using a combination of high coverage of Illumina and Nanopore reads, coupled with Hi-C and RNA-Seq data. Our genome assembly spans 1,007 Mb with a scaffold N50 of 11.38 Mb. We successfully anchored and oriented 98.6% of the genome on 22 linkage groups (LGs). Based on re-sequencing data for male and female fishes from three families, O. mossambicus segregates both an XY system on LG14 and a ZW system on LG3. The sex-patterned SNPs shared by two XY families narrowed the sex determining regions to ∼3 Mb on LG14. The shared sex-patterned SNPs included two deleterious missense mutations in ahnak and rhbdd1, indicating the possible roles of these two genes in sex determination. This annotated chromosome-level genome assembly and identification of sex determining regions represents a valuable resource to help understand the evolution of genetic sex determination in tilapias.

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“…This phenomenon may have resulted from an imprinting effect or a loss of linkage between the SD gene and the 247 allele of maternal origin in On stock. It is not clear if the previously reported XX/XY SD system on LG1 associated with SNP ONI28137 (Palaiokostas et al 2013;Taslima et al 2021), and the effect of LG1-BYL018, are caused by the same genetic factor, since the distance between these markers is approximately 0.8 Mbp.…”

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confidence: 99%

All‐male production by marker‐assisted selection for sex determining loci of admixed Oreochromis niloticus and Oreochromis aureus stocks

et al. 2021

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Crossing Oreochromis niloticus (On) females with Oreochromis aureus (Oa) males results in allmale progeny that are essential for effective tilapia farming. However, a reproductive barrier between these species limits mating and mass-fry production. One approach to overcoming this barrier is to select parental stocks of mixed genetic backgrounds, which allow interspecific reproductive recognition, while closely maintaining the genetic profiles for sexdetermination (SD) of the respective purebred species. Here, we test this approach in a data set of 160 On 9 Oa spawns of 109 male and 100 female parents randomly collected from admixed stocks, and genotyped for microsatellite markers representing the known SD loci on linkage groups (LGs) 1, 3, and 23. Following crossbreeding, the most significant paternal effects on male proportions in progeny were found for LG1-BYL018 (P < 2 9 10 À32 ) and for LG3-UNH168 9 LG23-UNH898 interaction (P < 1 9 10 À17 ; R 2 = 0.98). Furthermore, a maternal effect for LG3-UNH168 (P < 9 9 10 À7 ) was associated with low female proportions in progeny (<7%), indicating a non-Mendelian effect on SD. Eighty-four males (77%) and 30 females (30%) were selected as parents, based on their genetic profiles for the SD loci that were associated with male production. Of these, 51 of 53 crosses produced all-male progeny, while two crosses had low female proportions in their progeny (<4%). This suggests that selection could be improved using the causative sequence variation underlying SD on LG3, since the large non-recombining block of the SD region in purebred Oa readily breaks down in hybrids. Nevertheless, marker-assisted selection for sex determining loci of admixed parental stocks may be used for all-male production.

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Evidence of two XX/XY sex-determining loci in the Stirling stock of Nile tilapia (Oreochromis niloticus)

Cited by 13 publications

References 55 publications

Association of genetic markers with sex determination in Thai red tilapia

Association of genetic markers with sex determination in Thai red tilapia

A Chromosome-Level Genome Assembly of Mozambique Tilapia (Oreochromis mossambicus) Reveals the Structure of Sex Determining Regions

All‐male production by marker‐assisted selection for sex determining loci of admixed Oreochromis niloticus and Oreochromis aureus stocks

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