Evidence of the synergistic interaction of honey bee pathogens Nosema ceranae and Deformed wing virus

Zheng, Huoqing; Gong, Haiming; Huang, Shaokang; Sohr, Alex; Hu, Fuliang; Chen, Yan Ping

doi:10.1016/j.vetmic.2015.02.003

Cited by 36 publications

(20 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar effects of other viruses were described previously (Carrillo-Tripp et al, 2016). In beekeeping practice, it is generally accepted that most diseases, which are not directly linked with mites, occur (Zheng, Gong, Huang, & Sohr, 2015). Here, we did not find indications for pronounced synergism between DWV and N. ceranae.…”

Section: Discussionsupporting

confidence: 87%

“…Unfortunately, not much is known about the localization and tissue tropism of LSV, which could help explain its association with the intestinal parasites. A recent study indicated that the presence of the intestinal parasite N. ceranae promotes the proliferation of DWV, especially under nutritional stress (Zheng, Gong, Huang, & Sohr, ). Here, we did not find indications for pronounced synergism between DWV and N. ceranae .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Seasonal dynamics and co‐occurrence patterns of honey bee pathogens revealed by high‐throughput RT‐qPCR analysis

D’Alvise

Seeburger

Gihring

et al. 2019

Ecology and Evolution

View full text Add to dashboard Cite

The health of the honey bee Apis mellifera is challenged by introduced parasites that interact with its inherent pathogens and cause elevated rates of colony losses. To elucidate co‐occurrence, population dynamics, and synergistic interactions of honey bee pathogens, we established an array of diagnostic assays for a high‐throughput qPCR platform. Assuming that interaction of pathogens requires co‐occurrence within the same individual, single worker bees were analyzed instead of collective samples. Eleven viruses, four parasites, and three pathogenic bacteria were quantified in more than one thousand single bees sampled from sixteen disease‐free apiaries in Southwest Germany. The most abundant viruses were black queen cell virus (84%), Lake Sinai virus 1 (42%), and deformed wing virus B (35%). Forager bees from asymptomatic colonies were infected with two different viruses in average, and simultaneous infection with four to six viruses was common (14%). Also, the intestinal parasites Nosema ceranae (96%) and Crithidia mellificae/Lotmaria passim (52%) occurred very frequently. These results indicate that low‐level infections in honey bees are more common than previously assumed. All viruses showed seasonal variation, while N. ceranae did not. The foulbrood bacteria Paenibacillus larvae and Melissococcus plutonius were regionally distributed. Spearman's correlations and multiple regression analysis indicated possible synergistic interactions between the common pathogens, particularly for black queen cell virus. Beyond its suitability for further studies on honeybees, this targeted approach may be, due to its precision, capacity, and flexibility, a viable alternative to more expensive, sequencing‐based approaches in nonmodel systems.

show abstract

Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Seasonal dynamics and co‐occurrence patterns of honey bee pathogens revealed by high‐throughput RT‐qPCR analysis

D’Alvise

Seeburger

Gihring

et al. 2019

Ecology and Evolution

View full text Add to dashboard Cite

show abstract

“…The order of infection seems to be important; prior N. ceranae infection inhibited subsequent DWV infection but this was not reciprocal, suggesting asymmetry in the competitive interaction between these pathogens (Doublet et al ., ). In another experiment, N. ceranae fed to emerging bees from a DWV‐infected colony appeared to accelerate DWV replication at early stages of viral infection in a dose‐dependent manner, but not once DWV titers reached a plateau (Zheng et al ., ). However, the discrepancies among these studies could be attributed to differences in the methodologies applied to determine the viral load, such as the analysis of a specific tissue or the whole bee.…”

Section: What Are the Major Effects Of N Ceranae On Honey Bees?mentioning

confidence: 99%

Nosema ceranae in Apis mellifera: a 12 years postdetection perspective

Martín-Hernández

Bartolomé

Chejanovsky

et al. 2018

Environmental Microbiology

150

118

View full text Add to dashboard Cite

Nosema ceranae is a hot topic in honey bee health as reflected by numerous papers published every year. This review presents an update of the knowledge generated in the last 12 years in the field of N. ceranae research, addressing the routes of transmission, population structure and genetic diversity. This includes description of how the infection modifies the honey bee's metabolism, the immune response and other vital functions. The effects on individual honey bees will have a direct impact on the colony by leading to losses in the adult's population. The absence of clear clinical signs could keep the infection unnoticed by the beekeeper for long periods. The influence of the environmental conditions, beekeeping practices, bee genetics and the interaction with pesticides and other pathogens will have a direct influence on the prognosis of the disease. This review is approached from the point of view of the Mediterranean countries where the professional beekeeping has a high representation and where this pathogen is reported as an important threat.

show abstract

“…There are e.g. synergic effects between N. ceranae and DWV on honeybee health, which is dependent, in some cases, on pathogen titers and pollen nutrition (Zheng et al, 2015). Similarly, there were only seven cases (7/467) of mixed infections of N. ceranae and L. passim (Figure 4).…”

Section: Resultsmentioning

confidence: 98%

Viral and intestinal diseases detected in Apis mellifera in Central and Southern Chile

Vargas

Arismendi

Riveros

et al. 2017

Chilean J. Agric. Res.

View full text Add to dashboard Cite

Evidence of the synergistic interaction of honey bee pathogens Nosema ceranae and Deformed wing virus

Cited by 36 publications

References 38 publications

Seasonal dynamics and co‐occurrence patterns of honey bee pathogens revealed by high‐throughput RT‐qPCR analysis

Seasonal dynamics and co‐occurrence patterns of honey bee pathogens revealed by high‐throughput RT‐qPCR analysis

Nosema ceranae in Apis mellifera: a 12 years postdetection perspective

Viral and intestinal diseases detected in Apis mellifera in Central and Southern Chile

Contact Info

Product

Resources

About