Evidence of the Role of QTL Epistatic Interactions in the Increase of Melon Fruit Flesh Content during Domestication

Riahi, Chaymaa; Reig-Valiente, Juan Luis; Picó, Belén; Díaz, Aurora; Gonzalo, María José; Monforte, Antonio J.

doi:10.3390/agronomy10081064

Cited by 3 publications

(5 citation statements)

References 56 publications

(53 reference statements)

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“…Melon, a popular, horticultural crop, exhibits significant phenotypic variations. DNA-based genetic markers in the last few decades has been used to elucidate fruit quality traits in watermelon, melon and cucumber [ 35 , 36 , 37 , 38 , 39 ]. In this present study, we constructed a genetic linkage map using F 2 mapping populations over a three-year period at three distinct locations using genome-wide SNP-CAPS markers, and genetically mapped putative loci contributing to melon traits, such as exocarp and pericarp firmness and SSC ( Figure 5 ).…”

Section: Discussionmentioning

confidence: 99%

Genetic Mapping and QTL Analysis of Fruit Traits in Melon (Cucumis melo L.)

Zhao

Zhang

Meng

et al. 2023

CIMB

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Melon (Cucumis melo L.) is an important horticultural cash crop and its quality traits directly affect consumer choice and market price. These traits are controlled by genetic as well as environmental factors. In this study, a quantitative trait locus (QTL) mapping strategy was used to identify the potential genetic loci controlling quality traits of melons (i.e., exocarp and pericarp firmness and soluble solid content) based on newly derived whole-genome single nucleotide polymorphism-based cleaved amplified polymorphic sequence (SNP-CAPS) markers. Specifically, SNPs of two melon varieties, M4-5 and M1-15, as revealed by whole-genome sequencing, were converted to the CAPS markers, which were used to construct a genetic linkage map comprising 12 chromosomes with a total length of 1414.88 cM, in the F2 population of M4-5 and M1-15. The six identified QTLs included: SSC6.1 and SSC11.1 related to soluble solid content; EF12.1 associated with exocarp firmness; and EPF3.1, EPF3.2 and EPF7.1 related to edible pericarp firmness. These genes were located on five chromosomes (3, 6, 7, 11, and 12) in the flanking regions of the CAPS markers. Moreover, the newly developed CAPS markers will be useful in guiding genetic engineering and molecular breeding in melon.

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Section: Discussionmentioning

confidence: 99%

Genetic Mapping and QTL Analysis of Fruit Traits in Melon (Cucumis melo L.)

Zhao

Zhang

Meng

et al. 2023

CIMB

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“…Three QTLs were also detected for FFP (PA in the previous work) that mapped in the TRI05-2, TRI06-2 and TRI08-2 introgressions. Riahi et al [ 10 ] attempted to fine-map the FFP QTL on TRI06-2, but the results were inconsistent due to an epistatic interaction with a region in chromosome 11. In the current report, we have not found any consistent effects on FFP in any of the chromosome 11 ILs, confirming that there is not any additive QTL in that region.…”

Section: Discussionmentioning

confidence: 99%

“…Regarding edible flesh content, two QTLs that decreased the trait were mapped on chromosomes 6 and 8. An epistatic QTL on chromosome 11 was also identified in a subsequent work [ 10 ]. Zhao et al [ 6 ] also detected FW QTLs in the same regions of chromosomes 6 and 8, and additional QTLs on chromosomes 3, 5, and 7 in crosses between wild and cultivated melons.…”

Section: Introductionmentioning

confidence: 89%

“…To fully exploit the potential of wild and un-adapted genetic variability, it is necessary to perform genetic experiments in advanced populations [ 14 ]. Thus, the fruits of the PS-x-TRI F 2 are still very small compared with the cultivated PS (F 2 FW mean ranged from 186 to 245 g, whereas PS mean ranges between 1300 to 2485 g), and epistatic interactions between TRI alleles from different loci may frequent [ 10 , 15 ]. An efficient way to manage exotic germplasm is the construction of genetic libraries of introgression lines (ILs).…”

Section: Introductionmentioning

confidence: 99%

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A Novel Introgression Line Library Derived from a Wild Melon Gives Insights into the Genetics of Melon Domestication, Uncovering New Genetic Variability Useful for Breeding

Campos

Gonzalo

Díaz

et al. 2023

IJMS

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A collection of 30 melon introgression lines (ILs) was developed from the wild accession Ames 24297 (TRI) into ‘Piel de Sapo’ (PS) genetic background. Each IL carried an average of 1.4 introgressions from TRI, and the introgressions represented 91.4% of the TRI genome. Twenty-two ILs, representing 75% of the TRI genome, were evaluated in greenhouse (Algarrobo and Meliana) and field (Alcàsser) trials, mainly to study traits related to domestication syndrome such as fruit weight (FW) and flesh content (FFP), as well as other fruit quality traits as fruit shape (FS), flesh firmness (FF), soluble solid concentration (SSC), rind color and abscission layer. The IL collection showed an impressive variation in size-related traits, with FW ranging from 800 to 4100 g, reflecting the strong effect of the wild genome on these traits. Most of the ILs produced smaller fruits compared with PS; however, unexpectedly, the IL TRI05-2 produced bigger fruits, likely due to new epistatic interacions with the PS genetic background. In contrast, the genotypic effect for FS was smaller, and few QTLs with notable effects were detected. Interestingly, variability was also observed for FFP, FF and SSC, rind color and abscission layer formation. Genes in these introgressions are candidates for having been involved in melon domestication and diversification as well. These results confirm that the TRI IL collection is a very powerful tool for mapping traits of agronomic interest in melon, allowing the confirmation of previously reported QTLs and the identification of new ones to better understand the domestication process of this crop.

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“…Many of the interesting melon fruit traits, not to mention the increase in fruit flesh, are the major domestication achievements in this species, which originated from wild melons and produces small fruits with non-edible fruit flesh. Regarding this issue, Riahi et al [8] observed potential evidence of the involvement of QTL epistatic interactions in the increase in melon fruit flesh content occurring during melon domestication.…”

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confidence: 99%