Evidence of the involvement of spinal αB-crystallin in the maintenance of bone cancer pain in rats

Hang, Li-Hua; Chen, Hao Ming; Yu, Jian-Mang; Xu, Ying; Li, Shuna

doi:10.1007/s43440-019-00052-7

Cited by 1 publication

(1 citation statement)

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“…Recent studies found that alpha B-crystallin (CRYAB), a small heat shock protein that can reduce neuroinflammatory responses, exerts neuroinflammatory mechanisms and plays crucial roles in bone cancer pain (Hang et al, 2020). Moreover, the overexpression of CRYAB in cancer cell lines which was also seen in this study inhibits tumor cell migration and invasion (Ruan et al, 2020) and could be a novel biomarker candidate for a therapy in cervical carcinoma (Kori & Yalcin Arga, 2018).…”

Section: Discussionsupporting

confidence: 62%

The anticancer molecular mechanism of Carnosol in human cervical cancer cells: An in vitro study

Hafidh

Abdulamir

2020

APJMBB

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Carnosol, a phenolic diterpene, is one of the effective anticancer agents naturally occurring in rosemary, sage, parsley, and oregano. The chemoresistance problem increased with the routinely used chemotherapy. Therefore, the efforts to find a substitute with safe and low cost have become crucial worldwide. The current study attempts to inspect the anticancer molecular mechanisms of Carnosol on modulating up- and down- regulation of multiple genetic carcinogenesis pathways. The cytotoxicity of Carnosol on Hela cells was evaluated by MTS assay. Flow cytometry was used to assess apoptosis and cell cycle arrest. The apoptotic morphological changes were obvious by dual apoptosis assay. The differential gene expression after treatment with Carnosol was investigated by qRT-PCR. Up to 80% of the treated cells with Carnosol IC50 underwent apoptosis. Apoptosis together with cell cycle arrest in G0/G1 phase were induced significantly after treatment with Carnosol IC50. Fifteen out of nineteen genes studied were found to be remarkably up- or down- regulated after treatment with Carnosol. Six up-regulated genes (EREG, FOS-2, ID2, CRYAB, DUSP5, and TICAM2) and nine down-regulated genes (FN1, KRAS2, CCNB1-1, FEN1, MCM4, MCM5, GTSE1, CXCL1, and RALA) were recorded. These genes are candidates for future research for elucidating anticancer molecular targeted therapies, cancerous signaling and cancer development pathways in cervical cancer; moreover, elucidating the role of apoptosis, inflammation, cell proliferation, and cell differentiation in the development of cervical cancer.

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Section: Discussionsupporting

confidence: 62%