2004
DOI: 10.1657/1523-0430(2004)036[0370:eosdid]2.0.co;2
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Evidence of Sublethal Damage in Daphnia (Cladocera) during Exposure to Solar UV Radiation in Subarctic Ponds

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Cited by 27 publications
(28 citation statements)
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“…Analysis included three procedures: gravimetric extraction, derivatization, and quantification on a gas chromatograph (GC) following the methods described in Zellmer et al (2004) with the exception of the following. The bulk lipid extracts were applied to pre-conditioned Sep-Paks (Waters Silica cartridges, #WAT023537) and, following a series of pre-extractions, the phospholipid fraction was eluted and collected in the final wash and then dried under nitrogen gas for later GC analyses.…”
Section: Fatty Acid Analysismentioning
confidence: 99%
“…Analysis included three procedures: gravimetric extraction, derivatization, and quantification on a gas chromatograph (GC) following the methods described in Zellmer et al (2004) with the exception of the following. The bulk lipid extracts were applied to pre-conditioned Sep-Paks (Waters Silica cartridges, #WAT023537) and, following a series of pre-extractions, the phospholipid fraction was eluted and collected in the final wash and then dried under nitrogen gas for later GC analyses.…”
Section: Fatty Acid Analysismentioning
confidence: 99%
“…Analysis included three procedures: gravimetric extraction, derivitization, and quantification on a gas chromatograph (GC) following the methods described in Zellmer et al (2004). Fatty acid methyl esters (FAME) were identified using Supelco's 37 component FAME standard (#47885-U) by comparing peak retention times between samples and standards.…”
Section: Fatty Acidsmentioning
confidence: 99%
“…The procedures used were those of Zellmer et al [34], with the following modiWcations: helium was used as the carrier gas. Results are reported as g FAME mg ¡1 of dry biomass (db).…”
Section: Fatty Acid Compositionmentioning
confidence: 99%