Evidence of Resistance to Cry34/35Ab1 Corn by Western Corn Rootworm (Coleoptera: Chrysomelidae): Root Injury in the Field and Larval Survival in Plant-Based Bioassays

Gassmann, Aaron J.; Shrestha, Rajan; Jakka, Siva R. K.; Dunbar, Mike W.; Clifton, Eric H.; Paolino, Aubrey R.; Ingber, David A.; French, B. Wade; Masloski, Kenneth E.; Dounda, John W.; Clair, Coy R. St.

doi:10.1093/jee/tow110

Cited by 93 publications

(130 citation statements)

References 38 publications

Supporting

Mentioning

123

Contrasting

Order By: Relevance

“…Under the expectation of eventual selection or resistance evolution, an increase in Cry34Ab1/Cry35Ab1 product adoption accentuates the need for growers to follow best management practices for corn rootworm that reduce insect feeding pressure. Gassmann et al (2016) recently described a field population with “incomplete resistance” to Cry34Ab1/Cry35Ab1. Despite the high feeding pressure at most of the trial sites, Cry34Ab1/Cry35Ab1-expressing hybrids continued to provide an expected level of control below one node consumed on single trait technology (Herculex XTRA) and below 0.5 on the NIS for the pyramided technology (SmartStax) technologies in all 43 site-years of this study.…”

Section: Discussionmentioning

confidence: 99%

Field Trial Performance of Herculex XTRA (Cry34Ab1/Cry35Ab1) and SmartStax (Cry34Ab1/Cry35Ab1 + Cry3Bb1) Hybrids and Soil Insecticides Against Western and Northern Corn Rootworms (Coleoptera: Chrysomelidae)

Johnson¹,

Campbell²,

Lepping³

et al. 2017

Journal of Economic Entomology

View full text Add to dashboard Cite

Western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), and northern corn rootworm, Diabrotica barberi Smith and Lawrence (Coleoptera: Chrysomelidae), are important insect pests in corn, Zea mays L. For more than a decade, growers have been using transgenic plants expressing proteins from the bacterium Bacillus thuringiensis (Bt) to protect corn roots from feeding. In 2011, western corn rootworm populations were reported to have developed resistance to Bt hybrids expressing Cry3Bb1 and later found to be cross-resistant to hybrids expressing mCry3A and eCry3.1Ab. The identification of resistance to Cry3 (Cry3Bb1, mCry3A, and eCry3.1Ab) hybrids led to concerns about durability and efficacy of products with single traits and of products containing a pyramid of a Cry3 protein and the binary Bt proteins Cry34Ab1 and Cry35Ab1. From 2012 to 2014, 43 field trials were conducted across the central United States to estimate root protection provided by plants expressing Cry34Ab1/Cry35Ab1 alone (Herculex RW) or pyramided with Cry3Bb1 (SmartStax). These technologies were evaluated with and without soil-applied insecticides to determine if additional management measures provided benefit where Cry3 performance was reduced. Trials were categorized for analysis based on rootworm damage levels on Cry3-expressing hybrids and rootworm feeding pressure within each trial. Across scenarios, Cry34Ab1/Cry35Ab1 hybrids provided excellent root protection. Pyramided traits provided greater root and yield protection than non-Bt plus a soil-applied insecticide, and only in trials where larval feeding pressure exceeded two nodes of damage did Cry34Ab1/Cry35Ab1 single-trait hybrids and pyramided hybrids show greater root protection from the addition of soil-applied insecticides.

show abstract

Section: Discussionmentioning

confidence: 99%

Field Trial Performance of Herculex XTRA (Cry34Ab1/Cry35Ab1) and SmartStax (Cry34Ab1/Cry35Ab1 + Cry3Bb1) Hybrids and Soil Insecticides Against Western and Northern Corn Rootworms (Coleoptera: Chrysomelidae)

Johnson¹,

Campbell²,

Lepping³

et al. 2017

Journal of Economic Entomology

View full text Add to dashboard Cite

show abstract

“…Understanding the evolutionary dynamics of rotation resistance in D. v. virgifera is important because crop rotation is the favored tactic for mitigating the emerging problem of resistance to Bt corn [20,21]. How fast and how far Bt resistance may spread is of concern to farmers who must manage the risk of crop losses caused by a product that could start to fail.…”

Section: Spread Of Resistance Among Existing Populationsmentioning

confidence: 99%

Role of dispersal in resistance evolution and spread

Miller

Sappington

2017

Current Opinion in Insect Science

View full text Add to dashboard Cite

Gene flow via immigration affects rate of evolution of resistance to a pest management tactic, while emigration from a resistant population can spread resistance alleles spatially. Whether resistance detected across the landscape reflects ongoing de novo evolution in different hotspots or spread from a single focal population can determine the most effective mitigation strategy. Pest dispersal dynamics determine the spatio-temporal scale at which mitigation tactics must be applied to contain or reverse resistance in an area. Independent evolution of resistance in different populations appears common but not universal. Conversely, spatial spread appears to be almost inevitable. However, rate and scale of spread depends largely on dispersal dynamics and interplay with factors such as fitness costs, spatially variable selection pressure and whether resistance alleles are spreading through an established population or being carried by populations colonizing new territory. Disciplines Ecology and Evolutionary Biology | Entomology | Genetics CommentsThis article is published as Miller, Nicholas J. and Thomas W. Sappington. "Role of dispersal in resistance evolution and spread." Current opinion in insect science 21 (2017): 68-74.

show abstract

“…The goal of this study was to identify genes responsive in western corn rootworms challenged with its individual components as well as the binary toxin, Cry34/35Ab1 which has been commercialized in transgenic corn since 2006 and which still performs effectively in the field although incomplete resistance to Cry34/Cry35Ab1 was recently reported [19]. The objective of the present research is to develop an overview of WCR genes responsive to Bt Cry34/Cry35Ab1 intoxication.…”

Section: Introductionmentioning

confidence: 99%

Patterns of Gene Expression in Western Corn Rootworm (Diabrotica virgifera virgifera) Neonates, Challenged with Cry34Ab1, Cry35Ab1 and Cry34/35Ab1, Based on Next-Generation Sequencing

Wang

Eyun

Arora³

et al. 2017

Toxins

View full text Add to dashboard Cite

With Next Generation Sequencing technologies, high-throughput RNA sequencing (RNAseq) was conducted to examine gene expression in neonates of Diabrotica virgifera virgifera (LeConte) (Western Corn Rootworm, WCR) challenged with individual proteins of the binary Bacillus thuringiensis insecticidal proteins, Cry34Ab1 and Cry35Ab1, and the combination of Cry34/Cry35Ab1, which together are active against rootworm larvae. Integrated results of three different statistical comparisons identified 114 and 1300 differentially expressed transcripts (DETs) in the Cry34Ab1 and Cry34/35Ab1 treatment, respectively, as compared to the control. No DETs were identified in the Cry35Ab1 treatment. Putative Bt binding receptors previously identified in other insect species were not identified in DETs in this study. The majority of DETs (75% with Cry34Ab1 and 68.3% with Cry34/35Ab1 treatments) had no significant hits in the NCBI nr database. In addition, 92 DETs were shared between Cry34Ab1 and Cry34/35Ab1 treatments. Further analysis revealed that the most abundant DETs in both Cry34Ab1 and Cry34/35Ab1 treatments were associated with binding and catalytic activity. Results from this study confirmed the nature of these binary toxins against WCR larvae and provide a fundamental profile of expression pattern of genes in response to challenge of the Cry34/35Ab1 toxin, which may provide insight into potential resistance mechanisms.

show abstract

Evidence of Resistance to Cry34/35Ab1 Corn by Western Corn Rootworm (Coleoptera: Chrysomelidae): Root Injury in the Field and Larval Survival in Plant-Based Bioassays

Cited by 93 publications

References 38 publications

Field Trial Performance of Herculex XTRA (Cry34Ab1/Cry35Ab1) and SmartStax (Cry34Ab1/Cry35Ab1 + Cry3Bb1) Hybrids and Soil Insecticides Against Western and Northern Corn Rootworms (Coleoptera: Chrysomelidae)

Field Trial Performance of Herculex XTRA (Cry34Ab1/Cry35Ab1) and SmartStax (Cry34Ab1/Cry35Ab1 + Cry3Bb1) Hybrids and Soil Insecticides Against Western and Northern Corn Rootworms (Coleoptera: Chrysomelidae)

Role of dispersal in resistance evolution and spread

Patterns of Gene Expression in Western Corn Rootworm (Diabrotica virgifera virgifera) Neonates, Challenged with Cry34Ab1, Cry35Ab1 and Cry34/35Ab1, Based on Next-Generation Sequencing

Contact Info

Product

Resources

About