Evidence of reactive oxygen species (ROS) mediated apoptosis in Setaria cervi induced by green silver nanoparticles from Acacia auriculiformis at a very low dose

Saini, Prasanta; Saha, Swadhin K.; Roy, Priya; Chowdhury, Pranesh; Babu, Santi P. Sinha

doi:10.1016/j.exppara.2015.11.004

Cited by 57 publications

(37 citation statements)

References 31 publications

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“…The enhanced antibacterial activity of the irradiated samples is attributed to the production of excessive amount of reactive oxygen species (ROS) by the release metal ions [56,57]. These ROS triggers oxidative damage to various cellular components resulting cellular apoptosis [58,59]. Thus, the findings of this research verified the efficiency of TiO 2 and Ag/TiO 2 nanomaterials to eliminate E. coli and P.…”

Section: Antibacterial Activity Of Tio 2 Ag/tio 2 and Photo Irradiasupporting

confidence: 72%

Visible light photo catalytic inactivation of bacteria and photo degradation of methylene blue with Ag/TiO 2 nanocomposite prepared by a novel method

Tahir

Ahmad

et al. 2016

Journal of Photochemistry and Photobiology B: Biology

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Section: Antibacterial Activity Of Tio 2 Ag/tio 2 and Photo Irradiasupporting

confidence: 72%

Visible light photo catalytic inactivation of bacteria and photo degradation of methylene blue with Ag/TiO 2 nanocomposite prepared by a novel method

Tahir

Ahmad

et al. 2016

Journal of Photochemistry and Photobiology B: Biology

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“…The mechanism of cell death caused by NPs has been attributed to oxidative stress, with ROS generation indicated as a primary mechanism of NP related apoptotic cell death (Sastre et al 2000, Piao et al 2011, Yu et al 2013, Murphy et al 2015a, Saini et al 2016, Zhao et al 2016, Zhu et al 2016. ROS generation in the cells is regulated by protective enzymes and antioxidant mechanisms.…”

Section: Discussionmentioning

confidence: 99%

“…This triggers an inflammatory response and induces DNA damage within the cell and ultimately resulting in cell death (Nel et al 2006, Yu et al 2013, Murphy et al 2015b, Saini et al 2016.…”

Section: Discussionmentioning

confidence: 99%

In vitro comparative cytotoxicity study of aminated polystyrene, zinc oxide and silver nanoparticles on a cervical cancer cell line

Sharma

Gorey²,

Casey

2018

Drug and Chemical Toxicology

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Nanoparticles use in nano-biotechnology applications have increased significantly with Aminated polystyrene amine (AmPs NP), Zinc oxide (ZnO NP), and Silver (Ag NP) nanoparticles utilized in wide variety of consumer products. This has presented a number of concerns due to their increased exposure risks and associated toxicity on living systems. Changes in the structural and physicochemical properties of nanoparticles can lead to changes in biological activities. This study investigates, compares, and contrasts the potential toxicity of AmPs, ZnO and Ag NPs on an in vitro model (HeLa cells) and assesses the associated mechanism for their corresponding cytotoxicity relative to the surface material. It was noted that NPs exposure attributed to the reduction in cell viability and high-level induction of oxidative stress. All three test particles were noted to induce ROS to varying degrees which is irrespective of the attached surface group. Cell cycle analysis indicated a G2/M phase cell arrest, with the corresponding reduction in G0/G1 and S phase cells resulting in caspase-mediated apoptotic cell death. These findings suggest that all three NPs resulted in the decrease in cell viability, increase intracellular ROS production, induce cell cycle arrest at the G2/M phase and finally result in cell death by caspase-mediated apoptosis, which is irrespective of their differences in physiochemical properties and attached surface groups.ARTICLE HISTORY

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“…34,35 We thus reasoned that ZnO NP-mediated toxicity in MRC5 fibroblasts is caused by oxidative stress. Hence, we evaluated the oxidative stress induced by ZnO NP exposure by performing DCFDA staining, followed by flow cytometry to monitor the cellular …”

Section: Exposure To Zno Nps Causes Ros Induction and Increases The Ementioning

confidence: 99%

Zinc oxide nanoparticles exhibit cytotoxicity and genotoxicity through oxidative stress responses in human lung fibroblasts and <em>Drosophila melanogaster</em>

Yong

Hande³

et al. 2017

IJN

199

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Background Although zinc oxide nanoparticles (ZnO NPs) have been widely used, there has been an increasing number of reports on the toxicity of ZnO NPs. However, study on the underlying mechanisms under in vivo conditions is insufficient. Methods In this study, we investigated the toxicological profiles of ZnO NPs in MRC5 human lung fibroblasts in vitro and in an in vivo model using the fruit fly Drosophila melanogaster . A comprehensive study was conducted to evaluate the uptake, cytotoxicity, reactive oxygen species (ROS) formation, gene expression profiling and genotoxicity induced by ZnO NPs. Results For in vitro toxicity, the results showed that there was a significant release of extracellular lactate dehydrogenase and decreased cell viability in ZnO NP-treated MRC5 lung cells, indicating cellular damage and cytotoxicity. Generation of ROS was observed to be related to significant expression of DNA Damage Inducible Transcript ( DDIT3 ) and endoplasmic reticulum (ER) to nucleus signaling 1 ( ERN1 ) genes, which are ER stress-related genes. Oxidative stress induced DNA damage was further verified by a significant release of DNA oxidation product, 8-hydroxydeoxyguanosine (8-OHdG), as well as by the Comet assay. For the in vivo study using the fruit fly D. melanogaster as a model, significant toxicity was observed in F1 progenies upon ingestion of ZnO NPs. ZnO NPs induced significant decrease in the egg-to-adult viability of the flies. We further showed that the decreased viability is closely associated with ROS induction by ZnO NPs. Removal of one copy of the D. melanogaster Nrf2 alleles further decreased the ZnO NPs-induced lethality due to increased production of ROS, indicating that nuclear factor E2-related factor 2 (Nrf2) plays important role in ZnO NPs-mediated ROS production. Conclusion The present study suggests that ZnO NPs induced significant oxidative stress-related cytotoxicity and genotoxicity in human lung fibroblasts in vitro and in D. melanogaster in vivo. More extensive studies would be needed to verify the safety issues related to increased usage of ZnO NPs by consumers.

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Evidence of reactive oxygen species (ROS) mediated apoptosis in Setaria cervi induced by green silver nanoparticles from Acacia auriculiformis at a very low dose

Cited by 57 publications

References 31 publications

Visible light photo catalytic inactivation of bacteria and photo degradation of methylene blue with Ag/TiO 2 nanocomposite prepared by a novel method

Visible light photo catalytic inactivation of bacteria and photo degradation of methylene blue with Ag/TiO 2 nanocomposite prepared by a novel method

In vitro comparative cytotoxicity study of aminated polystyrene, zinc oxide and silver nanoparticles on a cervical cancer cell line

Zinc oxide nanoparticles exhibit cytotoxicity and genotoxicity through oxidative stress responses in human lung fibroblasts and <em>Drosophila melanogaster</em>

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