Evidence of presence and replication of honey bee viruses among wild bee pollinators in subtropical environments

Tapia-González, José María; Morfin, Nuria; Macías-Macías, José Octavio; Mora, Alvaro De la; Tapia-Rivera, José C.; Ayala, Ricardo; Contreras-Escareño, Francisca; Gashout, Hanan A.; Guzmán-Novoa, Ernesto

doi:10.1016/j.jip.2019.107256

Cited by 24 publications

(14 citation statements)

References 33 publications

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“…Furthermore, this, along with the general DWV variant similarity amongst samples means we cannot determine whether the samples were experiencing active infections. While the replication of DWV genotypes has been detected in other hosts, such as Varroa [41,42], ants (Formicidae) [43], stingless bees (Apidae) [44], solitary bees (Colletidae [44], Andrenidae [13] and Megachilidae [45]) and hornets (Vespidae) [46], and DWV has been detected in symptomatic (deformed) bumblebees (Bombus spp.) [47] and hornets (Vespa velutina) [48], conclusive studies on the ability of DWV to cause pathology in non-honey bee hosts have largely been limited to bumblebees [15,19].…”

Section: Discussionmentioning

confidence: 99%

RNAseq of Deformed Wing Virus and Other Honey Bee-Associated Viruses in Eight Insect Taxa with or without Varroa Infestation

Brettell

Schroeder

Martin

2020

Viruses

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The global spread of a parasitic mite (Varroa destructor) has resulted in Deformed wing virus (DWV), a previously rare pathogen, now dominating the viromes in honey bees and contributing to large-scale honey bee colony losses. DWV can be found in diverse insect taxa and has been implicated in spilling over from honey bees into associated (“apiary”) and other (“non-apiary”) insects. Here we generated next generation sequence data from 127 insect samples belonging to diverse taxa collected from Hawaiian islands with and without Varroa to identify whether the mite has indirectly affected the viral landscapes of key insect taxa across bees, wasps, flies and ants. Our data showed that, while Varroa was associated with a dramatic increase in abundance of (predominantly recombinant) DWV in honey bees (and no other honey bee-associated RNA virus), this change was not seen in any other taxa sampled. Honey bees share their environment with other insect populations and exist as a homogenous group, frequently sharing common viruses, albeit at low levels. Our data suggest that the threat of Varroa to increase viral load in an apiary does not automatically translate to an increase in virus load in other insects living in the wider community.

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Section: Discussionmentioning

confidence: 99%

RNAseq of Deformed Wing Virus and Other Honey Bee-Associated Viruses in Eight Insect Taxa with or without Varroa Infestation

Brettell

Schroeder

Martin

2020

Viruses

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“…This sampling site was dominated by Hirschfeldia incana (Mediterranean mustard) and a high degree of honey bee activity ( Figure 1 and Supplementary Table S1). Deformed wing virus strains commonly infect honey bees, replicate in and are transmitted by Varroa destructor mites that parasitize honey bees, and infect native and wild bee species and other insects [52,55,65,79,80,86,88,140,141]. Infection of developing honey bees may result in wing deformities that result in death during or shortly after emergence, whereas viral load and the ill effects of virus infection vary in adults, which can harbor between 1 × 10 5 to > 5 × 10 12 viral RNA copies per bee (estimated from qPCR values >2 × 10 9 copes/100 ng RNA and a total RNA per bee ~50 mg) [61,89,91,142,143,144].…”

Section: Black Queen Cell Virusmentioning

confidence: 99%

“…The most prevalent route of virus transmission between different bee taxa is likely via shared floral resources [53,56,81,82,83,84,85,86,87]. Specifically, infected bees can deposit viruses on flowers as they forage, and these viruses can then infect other bees that visit these flowers.…”

Section: Introductionmentioning

confidence: 99%

Metatranscriptome Analysis of Sympatric Bee Species Identifies Bee Virus Variants and a New Virus, Andrena-Associated Bee Virus-1

Daughenbaugh

Kahnonitch

Carey

et al. 2021

Viruses

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Bees are important plant pollinators in agricultural and natural ecosystems. High average annual losses of honey bee (Apis mellifera) colonies in some parts of the world, and regional population declines of some mining bee species (Andrena spp.), are attributed to multiple factors including habitat loss, lack of quality forage, insecticide exposure, and pathogens, including viruses. While research has primarily focused on viruses in honey bees, many of these viruses have a broad host range. It is therefore important to apply a community level approach in studying the epidemiology of bee viruses. We utilized high-throughput sequencing to evaluate viral diversity and viral sharing in sympatric, co-foraging bees in the context of habitat type. Variants of four common viruses (i.e., black queen cell virus, deformed wing virus, Lake Sinai virus 2, and Lake Sinai virus NE) were identified in honey bee and mining bee samples, and the high degree of nucleotide identity in the virus consensus sequences obtained from both taxa indicates virus sharing. We discovered a unique bipartite + ssRNA Tombo-like virus, Andrena-associated bee virus-1 (AnBV-1). AnBV-1 infects mining bees, honey bees, and primary honey bee pupal cells maintained in culture. AnBV-1 prevalence and abundance was greater in mining bees than in honey bees. Statistical modeling that examined the roles of ecological factors, including floral diversity and abundance, indicated that AnBV-1 infection prevalence in honey bees was greater in habitats with low floral diversity and abundance, and that interspecific virus transmission is strongly modulated by the floral community in the habitat. These results suggest that land management strategies that aim to enhance floral diversity and abundance may reduce AnBV-1 spread between co-foraging bees.

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“…Honey bee viruses have been found in native stingless bees from Brazil (Ueira-Vieira et al, 2015) and Mexico (Guzman-Novoa et al, 2015). Surprisingly, although honey bee viruses have been found to replicate in Mexican stingless bees, no signs of disease have been found in colonies (Tapia-González et al, 2019;Morfin et al, 2020). Notably, a study conducted in the Brazilian island of Fernando de Noronha, found ubiquitous presence of deformed wing virus type C (DWV-C) in M. subnitida colonies, but rarity in A. mellifera, which suggested limited viral exchange between these two species (De Souza et al, 2019).…”

Section: The Ecological Impact Of Ahbs In Mexicomentioning

confidence: 99%

The Process and Outcome of the Africanization of Honey Bees in Mexico: Lessons and Future Directions

et al. 2020

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Evidence of presence and replication of honey bee viruses among wild bee pollinators in subtropical environments

Cited by 24 publications

References 33 publications

RNAseq of Deformed Wing Virus and Other Honey Bee-Associated Viruses in Eight Insect Taxa with or without Varroa Infestation

RNAseq of Deformed Wing Virus and Other Honey Bee-Associated Viruses in Eight Insect Taxa with or without Varroa Infestation

Metatranscriptome Analysis of Sympatric Bee Species Identifies Bee Virus Variants and a New Virus, Andrena-Associated Bee Virus-1

The Process and Outcome of the Africanization of Honey Bees in Mexico: Lessons and Future Directions

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