Evidence of Potato virus Y Spread through Post-Emergence Management Practices in Commercial Potato Fields

MacKenzie, Tyler D. B.; Arju, Irin; Gallagher, A.; Nie, Xianzhou; Singh, Mathuresh

doi:10.1007/s12230-018-9679-4

Cited by 7 publications

(3 citation statements)

References 25 publications

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“…In nature, PVY is mainly transmitted horizontally from plants to plants by a number of aphid species in a non-persistent manner, and vertically through infected potato seed tubers (Lacomme et al, 2017). Mechanical transmission caused by management practices (e.g., tractor movement during pesticide applications) has also been reported (MacKenzie et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Molecular Evolutionary Analysis of Potato Virus Y Infecting Potato Based on the VPg Gene

et al. 2019

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Potato virus Y (PVY) is an important plant pathogen infecting solanaceous crops, causing significant losses to global potato and tobacco production. Some aspects of the plant pathology and molecular biology of PVY have been studied intensively, but the evolutionary dynamics of this virus are poorly understood. Here, we performed a comprehensive set of rigorous evolutionary analyses using 177 nucleotide sequences of the viral genome linked protein (VPg) gene, which interacts with the plant eukaryotic translation initiation factor 4E (eIF4E). Our Bayesian analysis reveals that the VPg gene of PVY has been evolving at a rate of 5.60 × 10 –4 subs/site/year (95% credibility interval 3.35 × 10 –4 –8.17 × 10 –4 ), which is equivalent to those of other plant-infecting RNA viruses. We identified different evolutionary constraints on the two clades of PVY, clade N and clade O, whose diverge time were estimated at the year 1861 CE (95% credibility interval 1750–1948 CE). We also found that genetic variations were correlated with geographic regions, suggesting that the evolution of this pathogen is strongly affected by geographical associated factors. Taken together, the results of our study have potential implications for the control strategies of PVY.

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Section: Introductionmentioning

confidence: 99%

Molecular Evolutionary Analysis of Potato Virus Y Infecting Potato Based on the VPg Gene

et al. 2019

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“…Potato virus Y (PVY) (family Potyviridae ) was used as a target polyvalent analyte. PVY was selected as the viral antigen, because it is one of the important and dangerous plant viruses in the family Solanaceae [ 25 , 26 ]. Virions with a mass of more than 60 × 10 3 kDa have filament-like structures, the length is 680–900 nm, and the diameter is 11–20 nm [ 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

“…In potato, PVY can induce a range of different symptoms, the most common being a leaf mosaic. However, the virus can also cause more severe effects, and is the causal agent of potato tuber necrotic ringspot disease [ 25 , 26 ]. An infected plant with mild symptoms of infection usually contains PVY RNA copies in the range from 2.4 × 10 9 per 1 mg of freeze-dried potato leaves [ 29 ], which is approximately 100 ng of PVY.…”

Section: Introductionmentioning

confidence: 99%

How to Improve Sensitivity of Sandwich Lateral Flow Immunoassay for Corpuscular Antigens on the Example of Potato Virus Y?

Razo

Panferov

Safenkova

et al. 2018

Sensors

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A simple approach was proposed to decrease the detection limit of sandwich lateral flow immunoassay (LFIA) by changing the conditions for binding between a polyvalent antigen and a conjugate of gold nanoparticles (GNPs) with antibodies. In this study, the potato virus Y (PVY) was used as the polyvalent antigen, which affects economically important plants in the Solanaceae family. The obtained polyclonal antibodies that are specific to PVY were characterized using a sandwich enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR). For LFIA, the antibodies were conjugated with GNPs with a diameter of 17.4 ± 1.0 nm. We conducted LFIAs using GNP conjugates in a dried state on the test strip and after pre-incubation with a sample. Pre-incubating the GNP conjugates and sample for 30 s was found to decrease the detection limit by 60-fold from 330 ng∙mL−1 to 5.4 ng∙mL−1 in comparison with conventional LFIA. The developed method was successfully tested for its ability to detect PVY in infected and uninfected potato leaves. The quantitative results of the proposed LFIA with pre-incubation were confirmed by ELISA, and resulted in a correlation coefficient of 0.891. The proposed approach is rapid, simple, and preserves the main advantages of LFIA as a non-laboratory diagnostic method.

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Epidemiology and Management of Potato Virus Y

MacKenzie

Nie

Singh

2020

Emerging Trends in Plant Pathology

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Evidence of Potato virus Y Spread through Post-Emergence Management Practices in Commercial Potato Fields

Cited by 7 publications

References 25 publications

Molecular Evolutionary Analysis of Potato Virus Y Infecting Potato Based on the VPg Gene

Molecular Evolutionary Analysis of Potato Virus Y Infecting Potato Based on the VPg Gene

How to Improve Sensitivity of Sandwich Lateral Flow Immunoassay for Corpuscular Antigens on the Example of Potato Virus Y?

Epidemiology and Management of Potato Virus Y

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