Evidence of Netosis in Septic Shock-Induced Disseminated Intravascular Coagulation

Delabranche, Xavier; Stiel, Laure; Séverac, François; Galoisy, Anne-Cécile; Mauvieux, Laurent; Zobairi, Fatiha; Lavigne, Thierry; Toti, Florence; Anglès-Cano, Eduardo; Meziani, Ferhat; Boisramé-Helms, Julie

doi:10.1097/shk.0000000000000719

Cited by 93 publications

(89 citation statements)

References 35 publications

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“…Despite the antimicrobial activity and tissue damage process during sepsis, NETs have also been suggested to contribute to coagulation disturbance in sepsis in recent studies . NETs were found to stimulate fibrin formation and deposition in vitro , and fibrin can colocalizes with NETs in blood clots .…”

Section: Alteration Of Neutrophil Function In Sepsismentioning

confidence: 99%

Neutrophil dysregulation during sepsis: an overview and update

Shen

Cao

Jiang

et al. 2017

J Cellular Molecular Medi

234

203

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Sepsis remains a leading cause of death worldwide, despite advances in critical care, and understanding of the pathophysiology and treatment strategies. No specific therapy or drugs are available for sepsis. Neutrophils play a critical role in controlling infection under normal conditions, and it is suggested that their migration and antimicrobial activity are impaired during sepsis which contribute to the dysregulation of immune responses. Recent studies further demonstrated that interruption or reversal of the impaired migration and antimicrobial function of neutrophils improves the outcome of sepsis in animal models. In this review, we provide an overview of the associated mediators and signal pathways involved which govern the survival, migration and antimicrobial function of neutrophils in sepsis, and discuss the potential of neutrophils as a target to specifically diagnose and/or predict the outcome of sepsis.

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Section: Alteration Of Neutrophil Function In Sepsismentioning

confidence: 99%

Neutrophil dysregulation during sepsis: an overview and update

Shen

Cao

Jiang

et al. 2017

J Cellular Molecular Medi

234

203

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“…In a recent multicenter study in patients presenting with stent thrombosis, neutrophils were the more abundant leukocyte population in thrombus specimens, whereas NETs were identified in 23% of thrombi (71). Regarding specific disorders associated with thrombotic manifestations, NETs in thrombus specimens and/or increased levels of nucleosomes have been identified in disseminated intravascular coagulation in sepsis (73), in paroxysmal nocturnal hemoglobinuria (81), thrombotic microangiopathies (82), antiphospholipid syndrome (APS) (74), antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) (14), or hemodialysis-related thrombogenicity (83). These clinical data support a role for NETs in the development of both arterial and venous thrombosis.…”

Section: Can Net Cargo Define Neutrophil Role In Disease?mentioning

confidence: 99%

NETopathies? Unraveling the Dark Side of Old Diseases through Neutrophils

et al. 2017

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Neutrophil extracellular traps (NETs) were initially described as an antimicrobial mechanism of neutrophils. Over the last decade, several lines of evidence support the involvement of NETs in a plethora of pathological conditions. Clinical and experimental data indicate that NET release constitutes a shared mechanism, which is involved in a different degree in various manifestations of non-infectious diseases. Even though the backbone of NETs is similar, there are differences in their protein load in different diseases, which represent alterations in neutrophil protein expression in distinct disorder-specific microenvironments. The characterization of NET protein load in different NET-driven disorders could be of significant diagnostic and/or therapeutic value. Additionally, it will provide further evidence for the role of NETs in disease pathogenesis, and it will enable the characterization of disorders in which neutrophils and NET-dependent inflammation are of critical importance.

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“…NETosis is involved in numerous pathological conditions involving neutrophil activation. Autoimmune disease, renal disease, thrombotic events, pulmonary disease, sepsis, and metastasis of cancer cells are examples of conditions in which NETosis has a pathological role (6,16,(25)(26)(27)(28)(29)(30)(31)(32)(33)(34). Therefore, a better understanding of the mechanisms mediating NETosis may lead to new pharmacological targets to reduce deleterious roles of NETs.…”

Section: -Chlorofatty Acids Induce Netosismentioning

confidence: 99%

2-Chlorofatty acids: lipid mediators of neutrophil extracellular trap formation

Palladino

Katunga

Kolar

et al. 2018

Journal of Lipid Research

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Neutrophils form neutrophil extracellular traps (NETs), which have been implicated in microcirculatory plugging. NET formation (NETosis) involves the fusion of granule and nuclear contents, which are then released in the extracellular space. Myeloperoxidase (MPO) plays a major role in NETosis leading to the dissociation of DNA from histones. During neutrophil activation, MPO is released and activated to convert hydrogen peroxide and chloride to hypochlorous acid (HOCl). HOCl targets plasmalogens leading to the production of the chlorinated lipids, 2-chlorofatty aldehyde and 2-chlorofatty acid (2-ClFA). Here, we tested the hypothesis that 2-ClFAs are important lipid mediators of NETosis. Human neutrophils treated with physiological levels of 2-ClFAs formed NETs, characterized by MPO association with DNA and neutrophil elastase (NE) redistribution to the perinuclear area. 2-ClFA-induced NETs reduced colony forming units. 2-ClFA-induced NETosis is calcium- and protein arginine deiminase 4-dependent. Interestingly, unlike PMA, 2-ClFA initiates the NETosis process without neutrophil activation and degranulation. Furthermore, 2-ClFA elicits NETosis in bone-marrow derived neutrophils from MPO-deficient mice. Taken together, these findings suggest 2-ClFA as an MPO product that triggers the NETosis pathway following neutrophil activation.

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Evidence of Netosis in Septic Shock-Induced Disseminated Intravascular Coagulation

Abstract: NEUT-SFL, NETs, and elevated nucleosome concentrations were all correlated to DIC (P <0.05). We have shown that NETosis is significantly correlated to septic shock-induced DIC.

Cited by 93 publications

References 35 publications

Neutrophil dysregulation during sepsis: an overview and update

Neutrophil dysregulation during sepsis: an overview and update

NETopathies? Unraveling the Dark Side of Old Diseases through Neutrophils

2-Chlorofatty acids: lipid mediators of neutrophil extracellular trap formation

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