Evidence of multiple sugar uptake across the plasma membrane of laticifer protoplasts from Hevea

Bouteau, François; Dellis, Olivier; Bousquet, U.; Rona, Jean‐Pierre

doi:10.1016/s0302-4598(98)00214-1

Cited by 23 publications

(17 citation statements)

References 17 publications

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“…7a). Palatinose, a nonmetabolizable sucrose analog, cannot be imported into plant cells (Bouteau et al 1999). The effect of palatinose (100 mM) on OsSUT2 gene expression was similar to that of sucrose (100 mM) (Fig.…”

Section: Expression Of Ossut2 Regulated By Exogenous Sugarsmentioning

confidence: 76%

Characterization of OsSUT2 Expression and Regulation in Germinating Embryos of Rice Seeds

Siao

Chen

Hsiao

et al. 2011

Rice

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OsSUT2 encodes a putative sucrose transporter containing 12 transmembrane domains in rice plants. Subcellular localization of the OsSUT2::GFP fusion protein indicated that OsSUT2 is a cell membrane protein. In embryos of germinating seeds, the expression of OsSUT2 gradually increased during the early germinating stage. The developmental regulations of OsSUT2 in germinating embryos could be mediated by sugars transported from endosperms. OsSUT2 expression was up-regulated by glucose through a hexokinase-independent pathway. Exogenous sucrose was sensed by a sensor localized on the plasma membrane and functioned as an enhancer to promote OsSUT2 expression. Based on OsSUT2 promoter::GUS expression in germinating seeds of transgenic rice, OsSUT2 was significantly expressed in the embryos and aleurone layers. In embryos, strong GUS expression was detected in the scutellum and vascular bundle tissues. Developmental stage-and sugar-dependent OsSUT2 expression was suggested to be controlled by transcriptional regulation of the promoter region.

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Section: Expression Of Ossut2 Regulated By Exogenous Sugarsmentioning

confidence: 76%

Characterization of OsSUT2 Expression and Regulation in Germinating Embryos of Rice Seeds

Siao

Chen

Hsiao

et al. 2011

Rice

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“…Therefore, evidence that the tip is inserted into the vacuole must be proven with membrane potential and obtained pH values (Felle 1993, Miller et al 2001). However, previously reported membrane potentials for plant protoplasts varied; negative values as intact cells (Abe et al 1983, Abe andTakeda 1986), low values near zero (Pantoja and Willmer 1986, Bouteau et al 1993, Bouteau et al 1999, Duijn and Hemimovaara-Dijkstra 1994, Shabala et al 1998 and positive values (Racusen et al 1977). The positive potential may be caused by membrane damage during enzymatic treatment (Cornel et al 1983).…”

Section: Vacuolar Ph Measurement Of Colored Cells Of Hydrangeamentioning

confidence: 96%

Sepal Color Variation of Hydrangea macrophylla and Vacuolar pH Measured with a Proton-Selective Microelectrode

Yoshida

Toyama-Kato

Kameda

et al. 2003

Plant and Cell Physiology

122

101

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;Sepal color of hydrangea varies with the environmental conditions. Although chemical and biological studies on this color variation have a long history, little correct knowledge has been generated about color development. All colored sepals contain the same anthocyanin, delphinidin 3-glucoside. Thus, there must be some other system for developing the wide variety of colors. In hydrangea sepals the cells of the epidermis are colorless and only the second layer of cells contain pigment. We prepared protoplasts without any color change during enzyme treatment of sepals and measured the vacuolar pH of each of the colored cells. We could correlate the color of a single hydrangea cell with its vacuolar pH using a combination of micro-spectrophotometry and a proton-selective microelectrode. Values for the vacuolar pH of blue (lvismax: 589 nm) and red cells (lvismax: 537 nm) were 4.1 and 3.3, respectively, the vacuolar pH of blue cells being significantly higher.

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“…Results from labelling experiments showed that V. zizanioides cell suspensions are able to uptake [U (14 C]-sucrose, [U (14 C]-D-glucose and [U (14 C]-fructose. The kinetics of sugar uptake into plant cells is frequently described as being biphasic, with a saturable component linked to an active transporter functioning at concentrations of a few millimolar, and a nonsaturable component linked to a facilitated diffusion at higher concentrations (Stanzel et al 1988;Bouteau et al 1999). In our experiment sucrose, glucose and fructose showed a biphasic uptake curve (data not .…”

Section: Discussionmentioning

confidence: 99%

Sugar metabolism ofVetiveria zizanioidescells cultured with Amberlite XAD-4. Uptake of radiolabelled sugars, cell wall invertase activity and gene expression

Mucciarelli¹,

Bertea

Camusso³

2009

Journal of Plant Interactions

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Activated and non-activated Amberlite XAD-4 was added to the cell cultures of vetiver (Vetiveria zizanioides Stapf.) in order to study its effects as abiotic growth promoter. Amberlite XAD-4 increased vetiver cell viability, especially when used in the activated form. Results from labelling experiments in cell suspensions showed that XAD-4 prompted a significant decrease of sucrose Km, accompanied by an increased Vmax. Glucose uptake from the medium was almost doubled, as demonstrated by the increase of the radiolabelled fraction (25-fold the controls) in vetiver cells cultured with XAD-4. Affinity for fructose uptake by cells was not altered by the use of activated XAD-4, even though its uptake and Vmax increased. These results were further supported by a significant 7.5-fold increase in cell wall invertases (CWI) activity in XAD-4 elicited cells. Vetiver CWI gene expression was characterized by transcript RT-PCR analysis. The identity of the transcripts was confirmed by sequencing analysis. These results indicated the use of XAD-4 as an effective tool for vetiver cell biomass optimization.

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Evidence of multiple sugar uptake across the plasma membrane of laticifer protoplasts from Hevea

Cited by 23 publications

References 17 publications

Characterization of OsSUT2 Expression and Regulation in Germinating Embryos of Rice Seeds

Characterization of OsSUT2 Expression and Regulation in Germinating Embryos of Rice Seeds

Sepal Color Variation of Hydrangea macrophylla and Vacuolar pH Measured with a Proton-Selective Microelectrode

Sugar metabolism ofVetiveria zizanioidescells cultured with Amberlite XAD-4. Uptake of radiolabelled sugars, cell wall invertase activity and gene expression

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