Evidence of multiple/cross resistance to Bt and organophosphate insecticides in Puerto Rico population of the fall armyworm, Spodoptera frugiperda

Zhu, Yu Cheng; Blanco, Carlos A.; Portilla, Maribel; Adamczyk, John J.; Luttrell, R. G.; Huang, Fangneng

doi:10.1016/j.pestbp.2015.01.007

Cited by 72 publications

(55 citation statements)

References 46 publications

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“…The binding data supported the absence of a critical Cry1Fa binding site in BBMV from 456 larvae, in agreement with previous reports suggesting reduced levels of diverse midgut proteins in larvae from 456 compared to those from susceptible insects (15,16). To identify specific Cry1Fa toxin receptors potentially involved in resistance, we cloned partial fragments of putative Cry1 receptor genes (17) from Ben larvae.…”

Section: Resultssupporting

confidence: 87%

“…Recent qualitative studies suggest an association between reduced binding and field-evolved resistance to TC1507 corn in S. frugiperda from Brazil (6). Reduced levels of diverse putative Cry1Fa toxin receptors were detected in strains of S. frugiperda from Puerto Rico with field-evolved resistance to TC1507 corn compared to the levels in susceptible insects (15,16), yet the mechanistic relevance of these differences to resistance was not addressed.…”

mentioning

confidence: 99%

“…In contrast, mode 1-type resistance to Bt sprays after field or greenhouse selection in Plutella xylostella and Trichoplusia ni, respectively, has been mapped to a multigene locus containing an ABC subfamily C2 transporter (ABCC2) gene (22). While no genetic linkage data are available, altered expression of ALP genes has been reported as being associated with resistance to Cry1 toxins in strains of H. virescens, H. armigera, Helicoverpa zea, and S. frugiperda (15,16,23). More recently, the finding that reduced expression of the mitogen-activated protein kinase 4 (MAPK4) gene trans-regulates reduced expression of the ALP and ABCC2 genes has been reported as being linked to resistance to Cry1Ac in diverse strains of P. xylostella (24).…”

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confidence: 99%

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Field-Evolved Mode 1 Resistance of the Fall Armyworm to Transgenic Cry1Fa-Expressing Corn Associated with Reduced Cry1Fa Toxin Binding and Midgut Alkaline Phosphatase Expression

Jakka

Gong

Hasler³

et al. 2016

Appl Environ Microbiol

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dInsecticidal protein genes from the bacterium Bacillus thuringiensis (Bt) are expressed by transgenic Bt crops (Bt crops) for effective and environmentally safe pest control. The development of resistance to these insecticidal proteins is considered the most serious threat to the sustainability of Bt crops. Resistance in fall armyworm (Spodoptera frugiperda) populations from Puerto Rico to transgenic corn producing the Cry1Fa insecticidal protein resulted, for the first time in the United States, in practical resistance, and Bt corn was withdrawn from the local market. In this study, we used a field-collected Cry1Fa corn-resistant strain (456) of S. frugiperda to identify the mechanism responsible for field-evolved resistance. Binding assays detected reduced Cry1Fa, Cry1Ab, and Cry1Ac but not Cry1Ca toxin binding to midgut brush border membrane vesicles (BBMV) from the larvae of strain 456 compared to that from the larvae of a susceptible (Ben) strain. This binding phenotype is descriptive of the mode 1 type of resistance to Bt toxins. A comparison of the transcript levels for putative Cry1 toxin receptor genes identified a significant downregulation (>90%) of a membrane-bound alkaline phosphatase (ALP), which translated to reduced ALP protein levels and a 75% reduction in ALP activity in BBMV from 456 compared to that of Ben larvae. We cloned and heterologously expressed this ALP from susceptible S. frugiperda larvae and demonstrated that it specifically binds with Cry1Fa toxin. This study provides a thorough mechanistic description of field-evolved resistance to a transgenic Bt crop and supports an association between resistance and reduced Cry1Fa toxin binding and levels of a putative Cry1Fa toxin receptor, ALP, in the midguts of S. frugiperda larvae.

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Section: Resultssupporting

confidence: 87%

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confidence: 99%

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confidence: 99%

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Field-Evolved Mode 1 Resistance of the Fall Armyworm to Transgenic Cry1Fa-Expressing Corn Associated with Reduced Cry1Fa Toxin Binding and Midgut Alkaline Phosphatase Expression

Jakka

Gong

Hasler³

et al. 2016

Appl Environ Microbiol

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“…A highly variable Cad protein (caused by alternative splicing) was found to associate with field-evolved resistance to Bt cotton in P. gossypiella [12], and a deletion mutation in the aminopeptidase N1 gene was related to Cry1Ac resistance in H. arimgera [13]. In addition, reduced abundance or binding of receptors for Cry1F has been found to associate with Cry1F resistance in S. frugiperda [14,15,16]. Furthermore, cellular signaling pathways and pore-formation may interact with each other in Bt resistance development.…”

Section: Introductionmentioning

confidence: 99%

Comparisons of Transcriptional Profiles of Gut Genes between Cry1Ab-Resistant and Susceptible Strains of Ostrinia nubilalis Revealed Genes Possibly Related to the Adaptation of Resistant Larvae to Transgenic Cry1Ab Corn

Yao

Zhu

et al. 2017

IJMS

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A microarray developed on the basis of 2895 unique transcripts from larval gut was used to compare gut gene expression profiles between a laboratory-selected Cry1Ab-resistant (R) strain and its isoline susceptible (S) strain of the European corn borer (Ostrinia nubilalis) after the larvae were fed the leaves of transgenic corn (MON810) expressing Cry1Ab or its non-transgenic isoline for 6 h. We revealed 398 gut genes differentially expressed (i.e., either up- or down-regulated genes with expression ratio ≥2.0) in S-strain, but only 264 gut genes differentially expressed in R-strain after being fed transgenic corn leaves. Although the percentages of down-regulated genes among the total number of differentially expressed genes (50% in S-strain and 45% in R-strain) were similar between the R- and S-strains, the expression ratios of down-regulated genes were much higher in S-strain than in R-strain. We revealed that 17 and 9 significantly up- or down-regulated gut genes from S and R-strain, respectively, including serine proteases and aminopeptidases. These genes may be associated with Cry1Ab toxicity by degradation, binding, and cellular defense. Overall, our study suggests enhanced adaptation of Cry1Ab-resistant larvae on transgenic Cry1Ab corn as revealed by lower number and lower ratios of differentially expressed genes in R-strain than in S-strain of O. nubilalis.

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“…The discovery of new substances as an alternative to synthetic insecticides is of great relevance, because the application of synthetic insecticides, besides causing adverse environmental effects, can result in the select for resistant insect populations, residue accumulation in food, and mortality of natural enemies (Busato et al, 2006;Souza et al, 2014;Zhu et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Malpighia emarginata DC. bagasse acetone extract: Phenolic compounds and their effecton Spodoptera frugiperda(J.E. Smith) (Lepidoptera: Noctuidae)

Marques

Caetano

Alves

et al. 2016

Chilean J. Agric. Res.

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Evidence of multiple/cross resistance to Bt and organophosphate insecticides in Puerto Rico population of the fall armyworm, Spodoptera frugiperda

Cited by 72 publications

References 46 publications

Field-Evolved Mode 1 Resistance of the Fall Armyworm to Transgenic Cry1Fa-Expressing Corn Associated with Reduced Cry1Fa Toxin Binding and Midgut Alkaline Phosphatase Expression

Field-Evolved Mode 1 Resistance of the Fall Armyworm to Transgenic Cry1Fa-Expressing Corn Associated with Reduced Cry1Fa Toxin Binding and Midgut Alkaline Phosphatase Expression

Comparisons of Transcriptional Profiles of Gut Genes between Cry1Ab-Resistant and Susceptible Strains of Ostrinia nubilalis Revealed Genes Possibly Related to the Adaptation of Resistant Larvae to Transgenic Cry1Ab Corn

Malpighia emarginata DC. bagasse acetone extract: Phenolic compounds and their effecton Spodoptera frugiperda(J.E. Smith) (Lepidoptera: Noctuidae)

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