Evidence of mitochondrial DNA clones of Siberian sturgeon, Acipenser baerii, within Russian sturgeon, Acipenser gueldenstaedtii, caught in the River Volga

Jenneckens, Ingo; Meyer, J. N.; Debus, L.; Pitra, Christian; Ludwig, Arne

doi:10.1046/j.1461-0248.2000.00179.x

Cited by 41 publications

(17 citation statements)

References 16 publications

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“…Hybrids between species for which no specific polymorphism was detected are not distinguishable, such as the ones between A. baerii and A. gueldenstaedtii observed in the Volga River (Jenneckens et al . ) and of increasing interest for aquaculture (Ludwig ).…”

Section: Resultsmentioning

confidence: 99%

Species and hybrid identification of sturgeon caviar: a new molecular approach to detect illegal trade

Boscari

Barmintseva

Pujolar

et al. 2014

Molecular Ecology Resources

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Overexploitation of wild populations due to the high economic value of caviar has driven sturgeons to near extinction. The high prices commanded by caviar on world markets have made it a magnet for illegal and fraudulent caviar trade, often involving low-value farmed caviar being sold as top-quality caviar. We present a new molecular approach for the identification of pure sturgeon species and hybrids that are among the most commercialized species in Europe and North America. Our test is based on the discovery of species-specific single nucleotide polymorphisms (SNPs) in the ribosomal protein S7, supplemented with the Vimentin gene and the mitochondrial D-loop. Test validations performed in 702 specimens of target and nontarget sturgeon species demonstrated a 100% identification success for Acipenser naccarii, A. fulvescens, A. stellatus, A. sinensis and A. transmontanus. In addition to species identification, our approach allows the identification of Bester and AL hybrids, two of the most economically important hybrids in the world, with 80% and 100% success, respectively. Moreover, the approach has the potential to identify many other existing sturgeon hybrids. The development of a standardized sturgeon identification tool will directly benefit trade law enforcement, providing the tools to monitor and regulate the legal trade of caviar and protect sturgeon stocks from illicit producers and traders, hence contributing to safeguarding this group of heavily threatened species.

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Section: Resultsmentioning

confidence: 99%

Species and hybrid identification of sturgeon caviar: a new molecular approach to detect illegal trade

Boscari

Barmintseva

Pujolar

et al. 2014

Molecular Ecology Resources

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“…gueldenstaedti led to the loss of the morphological diagnostic A . baerii features [33]. In our study, we also used nine microsatellite loci from nuclear DNA and mtDNA COI for sturgeon identification.…”

Section: Discussionmentioning

confidence: 99%

Genetic variation and relationships of seven sturgeon species and ten interspecific hybrids

Zhang

Li³

et al. 2013

Genet Sel Evol

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BackgroundSturgeon cultivation is important for both industry and aquaculture in China. To date, more than 17 species or strains have been farmed for fillets and caviar production. Crossbreeding among different sturgeon species is frequent and the F2 hybrids are fertile. However, large-scale farming can have negative impacts on wild populations i.e. escape of exotic sturgeons and must be taken into consideration. Escape of exotic sturgeons can cause severe ecological problems, including threatening native sturgeon species once the exotic varieties become established or hybridize with native individuals. However, little is known about their genetic resources and variation.MethodsGenetic diversity and introgression of seven sturgeon species were analyzed using mitochondrial DNA cytochrome oxidase subunit I (COI) and nine microsatellite markers. This study included 189 individuals from seven sturgeon species and 277 individuals from ten lineages of F2 hybrid strains.ResultsMtDNA COI sequences (632 bp long) were generated from 91 individuals across the 17 sturgeon strains and produced 23 different haplotypes. Haplotype diversity was high (h = 0.915 ± 0.015) and nucleotide diversity was low (π = 0.03680 ± 0.00153) in the seven sturgeon species and ten interspecific hybrids. Phylogenetic analyses resulted in almost identical tree topologies, and different haplotype structures were mainly related with sturgeons of different female parents. Analysis of molecular variance revealed that 81.73% of the genetic variance was due to matrilineal differences, while 9.40% resulted from strain variation. Pairwise Fst values obtained with POLYSAT software, were high among strains and ranged from 0.031 to 0.164. Admixture analysis assigned seven distinct groups and ten genotypes of admixed clusters composed of hybrid strains using STRUCTURE when assuming K = 7.ConclusionsThe interspecific mtDNA gene tree corresponded to the expected taxonomic divisions. These relationships were also supported by the results from the microsatellite analysis and contributed to unambiguously identify seven sturgeon species and ten F2 hybrid strains from sturgeon farms in China. Moreover, we found that introgressive hybridization is pervasive, exists in both purebred and hybrid sturgeons, and may reflect widespread mismanagement in sturgeon breeding in China.

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“…These species do not overlap in geographic distribution, with A. baerii inhabiting lakes and rivers of Siberia and A. gueldenstaedtii found in the Caspian and Black Sea regions. Introgression between the species occurs in the Caspian and Azov Sea where animals morphologically identified as A. gueldenstaedtii have mtDNA haplotypes similar to A. baerii , potentially as a result of release from aquaculture [18], [27]. Distinguishing between A. baerii and A. baerii-like gueldenstaedtii can be accomplished through the D-loop sequence-based method applied here [20].…”

Section: Discussionmentioning

confidence: 99%

Testing the Effectiveness of an International Conservation Agreement: Marketplace Forensics and CITES Caviar Trade Regulation

et al. 2012

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BackgroundThe international wildlife trade is a key threat to biodiversity. Temporal genetic marketplace monitoring can determine if wildlife trade regulation efforts such as the Convention on International Trade in Endangered Species (CITES) are succeeding. Protected under CITES effective 1997, sturgeons and paddlefishes, the producers of black caviar, are flagship CITES species.Methodology/Principal FindingsWe test whether CITES has limited the amount of fraudulent black caviar reaching the marketplace. Using mitochondrial DNA-based methods, we compare mislabeling in caviar and meat purchased in the New York City area pre and post CITES listing. Our recent sampling of this market reveals a decrease in mislabeled caviar (2006–2008; 10%; n = 90) compared to pre-CITES implementation (1995–1996; 19%; n = 95). Mislabeled caviar was found only in online purchase (n = 49 online/41 retail).Conclusions/SignificanceStricter controls on importing and exporting as per CITES policies may be having a positive conservation effect by limiting the amount of fraudulent caviar reaching the marketplace. Sturgeons and paddlefishes remain a conservation priority, however, due to continued overfishing and habitat degradation. Other marine and aquatic species stand to benefit from the international trade regulation that can result from CITES listing.

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Evidence of mitochondrial DNA clones of Siberian sturgeon, Acipenser baerii, within Russian sturgeon, Acipenser gueldenstaedtii, caught in the River Volga

Cited by 41 publications

References 16 publications

Species and hybrid identification of sturgeon caviar: a new molecular approach to detect illegal trade

Species and hybrid identification of sturgeon caviar: a new molecular approach to detect illegal trade

Genetic variation and relationships of seven sturgeon species and ten interspecific hybrids

Testing the Effectiveness of an International Conservation Agreement: Marketplace Forensics and CITES Caviar Trade Regulation

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