Evidence of metabolic shift on hydrogen, ethanol and 1,3-propanediol production from crude glycerol by nitrogen sparging under micro-aerobic conditions using co-culture of Enterobacter aerogenes and Clostridium butyricum

Pachapur, Vinayak Laxman; Brar, Satinder Kaur; Bihan, Yann Le; Buelna, Gerardo; Soccol, Carlos Ricardo

doi:10.1016/j.ijhydene.2015.05.024

Cited by 52 publications

(32 citation statements)

References 29 publications

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“…The supernatant was collected to determine the metabolite concentration. For all samples, isobutanol was mixed as an internal standard and the injection volume was 1.0 μL (Pachapur et al, 2015).…”

Section: Gas Chromatographic Analysismentioning

confidence: 99%

Toxicity of chlortetracycline and its metal complexes to model microorganisms in wastewater sludge

Pulicharla

Das

Brar

et al. 2015

Science of The Total Environment

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Section: Gas Chromatographic Analysismentioning

confidence: 99%

Toxicity of chlortetracycline and its metal complexes to model microorganisms in wastewater sludge

Pulicharla

Das

Brar

et al. 2015

Science of The Total Environment

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“…Fermentative production of 1,3‐PDO by microorganisms has been extensively studied and achieved success in batch, fed‐batch, repeated batch, and continuous fermentative systems in the past decades. Many species of microbes including the Clostridium, Klebsiella, Citrobacter, Enterobacter and even Shimwellia can convert crude glycerol to 1,3‐PDO. Among these microbes C. butyricum is thought to be one of the most prominent 1,3‐PDO producers owing to its exceptional and efficient performance in 1,3‐PDO production.…”

Section: Introductionmentioning

confidence: 99%

Adaptive evolution of Clostridium butyricum and scale‐Up for high‐Concentration 1,3‐propanediol production

et al. 2018

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In order to obtain the excellent 1,3-propanediol (1,3-PDO) producer from wild-type Clostridium butyricum, adaptive evolution was carried out to select the strain for fast growth. The most significant change was that fermentation time decreased from 36 h to 20 h after adaptive evolution. Thus, it led to the corresponding volumetric productivity of 1,3-PDO increasing from 0.97 to 2.14 (g/LÁh −1 ) which increased by 114%. Adaptive evolution was also applied to butyric acid tolerant strain selected based on the fast-growing one through a simple equipment. 1,3-PDO concentration increased from 40.28 to 66.23 g/L through fed-batch fermentation by butyric acid tolerant strain compared with the fast-growing one. In addition, the endpoint strain was successfully and steadily used in the 50-L scale fermentation. Thus, adaptive evolution is an excellent strategy which can help us select the fast-growing strain and reduce the negative effect from substrate and metabolite inhibition. (a) the morphology of Clostridium butyricum in the initial stage of Gen 0 adaptive evolution process. (b) The morphology of Clostridium butyricum in the last stage of Gen 0 adaptive evolution process. (c) The effect of 5 g/L butyric acid and acetic acid to the growth of Gen 4 strain. (d) Different growing status of Gen 0, Gen 1, Gen 2, Gen 3, and Gen 4 strain in seed culture contained 70 g/L glycerol. The "XMU" were written on the back of the each bottles. [Color figure can be viewed at wileyonlinelibrary.com] Figure 5. Time course of Gen 7 strain fermentation on 50-L scale. (a, b) Represent the concentrations of glycerol, 1,3-PDO (g/L), acids and OD in batch and fed-batch fermentation process.[Color figure can be viewed at wileyonlinelibrary.com]

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“…It enhanced the yield of 1,3-PDO from 0.5 to 3.0 g/L. This change was attributed to shifting from reductive to oxidative metabolism of glycerol with the co-culture of C. butyricum and Enterobacter aerogenes [29]. Naturally occurring microbes are always expected to perform the bioprocess in an economical manner.…”

Section: Propanediolsmentioning

confidence: 99%

Biorefinery for Glycerol Rich Biodiesel Industry Waste

2016

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The biodiesel industry has the potential to meet the fuel requirements in the future. A few inherent lacunae of this bioprocess are the effluent, which is 10 % of the actual product, and the fact that it is 85 % glycerol along with a few impurities. Biological treatments of wastes have been known as a dependable and economical direction of overseeing them and bring some value added products as well. A novel eco-biotechnological strategy employs metabolically diverse bacteria, which ensures higher reproducibility and economics. In this article, we have opined, which organisms and what bioproducts should be the focus, while exploiting glycerol as feed.

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Evidence of metabolic shift on hydrogen, ethanol and 1,3-propanediol production from crude glycerol by nitrogen sparging under micro-aerobic conditions using co-culture of Enterobacter aerogenes and Clostridium butyricum

Cited by 52 publications

References 29 publications

Toxicity of chlortetracycline and its metal complexes to model microorganisms in wastewater sludge

Toxicity of chlortetracycline and its metal complexes to model microorganisms in wastewater sludge

Adaptive evolution of Clostridium butyricum and scale‐Up for high‐Concentration 1,3‐propanediol production

Biorefinery for Glycerol Rich Biodiesel Industry Waste

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