Evidence of in vitro metabolic interaction effects of a chlorfenvinphos, ethion and linuron mixture on human hepatic detoxification rates

Abstract

idence of in vitro metabolic interaction effects of a chlorfenvinphos, ethion and linuron mixture on human hepatic detoxification rates. Chemosphere, Elsevier, 2017, 181, pp.666-674 General population exposure to pesticides mainly occurs via food and water consumption. However, 11 their risk assessment for regulatory purposes does not currently consider the actual co-exposure to 12 multiple substances. To address this concern, relevant experimental studies are needed to fill the lack 13 of data concerning effects of mixture on human health. For the first time, the present work evaluated 14 on human microsomes and liver cells the combined metabolic effects of, chlorfenvinphos, ethion and 15 linuron, three pesticides usually found in vegetables of the European Union. Concentrations of these 16 substances were measured during combined incubation experiments, thanks to a new analytical 17 methodology previously developed. The collected data allowed for calculation and comparison of the 18 intrinsic hepatic clearance of each pesticide from different combinations. Finally, the results showed 19 clear inhibitory effects, depending on the association of the chemicals at stake. The major metabolic 20 inhibitor observed was chlorfenvinphos. During co-incubation, it was able to decrease the intrinsic 21 clearance of both linuron and ethion. These latter also showed a potential for metabolic inhibition 22 mainly cytochrome P450-mediated in all cases. Here we demonstrated that human detoxification from 23 a pesticide may be severely hampered in case of co-occurrence of other pesticides, as it is the case for 24 drugs interactions, thus increasing the risk of adverse health effects. These results could contribute to 25

Effects of acaricides on the activities of monooxygenases in bovine liver microsomes

“…This metabolic reaction occurs in the liver of different species including cattle (Hodgson, 2003; Johnsen & Dahm, 1966). Different CYPs, mainly CYP1A2, CYP2B6 and CYP3A4, were shown to be involved in the production of oxon metabolites in the human liver (Foxenberg et al, 2006; Hodgson & Rose, 2006; Kadar et al, 2017; Leoni et al, 2008). To the best of our knowledge, there is a lack of information on the specific enzyme pathways involved in the metabolism of OPs in cattle.…”

Isolation and Characterization of Multipotent Turkey Tendon-Derived Stem Cells

“…Although the chicken model is more affordable and has similarity to human vasculature [ 31 , 32 ], it is complicated by an additional phalanx in the third digit and difficulties in postoperative rehabilitation [ 33 ]. Recently, our group has shown that turkey flexor tendons have many similarities such as anatomy and biomechanical properties to human flexor tendons, which would make the turkey a potential new large animal model for clinically relevant flexor tendon research [ 34 ]. The ability to identify turkey TDSCs would pave the way for studies on its role in tendon physiology and tendinopathy and open up new treatment for tendon diseases.…”