Evidence of hypervirulence in Listeria monocytogenes clonal complex 14

Cardenas-Alvarez, Maria X; Ramsett, Megan K. Townsend; Malekmohammadi, Sahar; Bergholz, Teresa M.

doi:10.1099/jmm.0.001076

Cited by 10 publications

(9 citation statements)

References 40 publications

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“…According to several authors, the other remaining CCs detected in this study were previously isolated from different sources, such as food and environments [10,45,46,48,[52][53][54][55][56][57].…”

Section: Distribution Of Ccs and Cg-mlst Clusters And Their Virulence And Stress Resistance Profilesmentioning

confidence: 72%

Intensive Environmental Surveillance Plan for Listeria monocytogenes in Food Producing Plants and Retail Stores of Central Italy: Prevalence and Genetic Diversity

Centorotola

Guidi

D'Aurizio³

et al. 2021

Foods

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Listeria monocytogenes (Lm) can persist in food processing environments (FPEs), surviving environmental stresses and disinfectants. We described an intensive environmental monitoring plan performed in Central Italy and involving food producing plants (FPPs) and retail grocery stores (RSs). The aim of the study was to provide a snapshot of the Lm circulation in different FPEs during a severe listeriosis outbreak, using whole genome sequencing (WGS) to investigate the genetic diversity of the Lm isolated, evaluating their virulence and stress resistance profiles. A total of 1217 samples were collected in 86 FPEs with 12.0% of positive surfaces at FPPs level and 7.5% at RSs level; 133 Lm isolates were typed by multilocus sequencing typing (MLST) and core genome MLST (cgMLST). Clonal complex (CC) 121 (25.6%), CC9 (22.6%), CC1 (11.3%), CC3 (10.5%), CC191 (4.5%), CC7 (4.5%) and CC31 (3.8%) were the most frequent MLST clones. Among the 26 cgMLST clusters obtained, 5 of them persisted after sanitization and were re-isolated during the follow-up sampling. All the CC121 harboured the Tn6188_qac gene for tolerance to benzalkonium chloride and the stress survival islet SSI-2. The CC3, CC7, CC9, CC31 and CC191 carried the SSI-1. All the CC9 and CC121 strains presented a premature stop codon in the inlA gene. In addition to the Lm Pathogenicity Island 1 (LIPI-1), CC1, CC3 and CC191 harboured the LIPI-3. The application of intensive environmental sampling plans for the detection and WGS analysis of Lm isolates could improve surveillance and early detection of outbreaks.

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Section: Distribution Of Ccs and Cg-mlst Clusters And Their Virulence And Stress Resistance Profilesmentioning

confidence: 72%

Intensive Environmental Surveillance Plan for Listeria monocytogenes in Food Producing Plants and Retail Stores of Central Italy: Prevalence and Genetic Diversity

Centorotola

Guidi

D'Aurizio³

et al. 2021

Foods

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“…Furthermore, multilocus sequence typing (MLST), based on seven housekeeping genes, is also a powerful and extensively-used epidemiological typing tool and allows for the classification of most clinical L. monocytogenes isolates into multiple clonal complexes (CCs) (Moura et al, 2016;Wu et al, 2016). In addition, L. monocytogenes can be classified into four distinct evolutionary lineages (denoted I to IV) with most isolates grouping into lineages I and II (Cardenas-Alvarez et al, 2019;Fan et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Listeriosis Cases and Genetic Diversity of Their L. monocytogenes Isolates in China, 2008–2019

Yang

Gao

et al. 2021

Front. Cell. Infect. Microbiol.

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Listeriosis, caused by Listeria monocytogenes, is a severe food-borne infection. The nationwide surveillance in China concerning listeriosis is urgently needed. In the present study, 144 L. monocytogenes isolates were collected from the samples of blood, cerebrospinal fluid (CSF), and fetal membrane/placenta in China for 12 years from 2008 to 2019. We summarized these listeriosis patients’ demographical and clinical features and outcomes. The susceptibility profile for 12 antibiotics was also determined by the broth microdilution method. Multilocus sequence typing (MLST) and serogroups of these listeria isolates were analyzed to designate epidemiological types. We enrolled 144 cases from 29 healthcare centers, including 96 maternal-neonatal infections, 33 cases of bacteremia, 13 cases of neurolisteriosis, and two cutaneous listeriosis. There were 31 (59.6%) fetal loss in 52 pregnant women and four (9.8%) neonatal death in 41 newborns. Among the 48 nonmaternal-neonatal cases, 12.5% (6/48) died, 41.7% (20/48) were female, and 64.6% (31/48) occurred in those with significant comorbidities. By MLST, the strains were distinguished into 23 individual sequence types (STs). The most prevalent ST was ST87 (49 isolates, 34.0%), followed by ST1 (18, 12.5%), ST8 (10, 6.9%), ST619 (9, 6.3%), ST7 (7, 4.9%) and ST3 (7, 4.9%). Furthermore, all L. monocytogenes isolates were uniformly susceptible to penicillin, ampicillin, and meropenem. In summary, our study highlights a high genotypic diversity of L. monocytogenes strains causing clinical listeriosis in China. Furthermore, a high prevalence of ST87 and ST1 in the listeriosis should be noted.

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“…CC14 and CC7, which were of special interest as they were found as hypervirulent and hypovirulent, respectively, in G. mellonella larvae [ 55 ] showed differences in virulence gene content when compared. Regarding pathogenicity islands, CC14 harbor only LIPI-1, while CC7 strains possessed LIPI-1 and SSI-1.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Study of Listeria monocytogenes Isolates Causing Three Different Clinical Outcomes

2022

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Heterogeneity in virulence potential of L. monocytogenes subgroups have been associated with genetic elements that could provide advantages in certain environments to invade, multiply, and survive within a host. The presence of gene mutations has been found to be related to attenuated phenotypes, while the presence of groups of genes, such as pathogenicity islands (PI), has been associated with hypervirulent or stress-resistant clones. We evaluated 232 whole genome sequences from invasive listeriosis cases in human and ruminants from the US and Europe to identify genomic elements associated with strains causing three clinical outcomes: central nervous system (CNS) infections, maternal-neonatal (MN) infections, and systemic infections (SI). Phylogenetic relationships and virulence-associated genes were evaluated, and a gene-based and single nucleotide polymorphism (SNP)-based genome-wide association study (GWAS) were conducted in order to identify loci associated with the different clinical outcomes. The orthologous results indicated that genes of phage phiX174, transfer RNAs, and type I restriction-modification (RM) system genes along with SNPs in loci involved in environmental adaptation such as rpoB and a phosphotransferase system (PTS) were associated with one or more clinical outcomes. Detection of phenotype-specific candidate loci represents an approach that could narrow the group of genetic elements to be evaluated in future studies.

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Evidence of hypervirulence in Listeria monocytogenes clonal complex 14

Cited by 10 publications

References 40 publications

Intensive Environmental Surveillance Plan for Listeria monocytogenes in Food Producing Plants and Retail Stores of Central Italy: Prevalence and Genetic Diversity

Intensive Environmental Surveillance Plan for Listeria monocytogenes in Food Producing Plants and Retail Stores of Central Italy: Prevalence and Genetic Diversity

Listeriosis Cases and Genetic Diversity of Their L. monocytogenes Isolates in China, 2008–2019

Genome-Wide Association Study of Listeria monocytogenes Isolates Causing Three Different Clinical Outcomes

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