Evidence of Grapevine leafroll associated virus-1–3, Grapevine fleck virus and Grapevine virus B Occurring in Himachal Pradesh, India

Kumar, Surender; Singh, Latika; Ferretti, L.; Barba, M.; Zaidi, A. A.; Hallan, Vipin

doi:10.1007/s13337-013-0129-0

Cited by 16 publications

(7 citation statements)

References 14 publications

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“…Nonetheless, this is the first study that could successfully detect GLRaV-2 or any of its variants in India. Though Kumar et al (2013) did attempt to detect this virus in India they could not succeed rather they detected GLRaV1 and GLRaV3. Further, GLRaV5 and GLRaV6 are presently regarded as the strains of GLRaV4 (Rai et al, 2017).…”

Section: Snp Detection and Recombination Analyses In Reconstructed Gementioning

confidence: 99%

“…Though India grows grapevine on 137,000 hectares and exports 185,172 tonnes of grapes annually (FAOSTAT, 2017), only a few studies have been attempted to detect grapevine viruses in India. All these studies targeted only one/few virus(es)/ viroid(s) at a time using traditional detection methods (Kumar et al, 2012(Kumar et al, , 2013Sahana et al, 2013;Adkar-Purushothama et al, 2014;Rai et al, 2017;Marwal et al, 2019;Singhal et al, 2019). The current study is the first virome report of grapevines from India using sRNA and mRNA datasets of three Indian grapevine cultivars available in the public domain (Tirumalai et al, 2019) identifying a large number of viruses and viroids.…”

Section: Introductionmentioning

confidence: 99%

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Robust Virome Profiling and Whole Genome Reconstruction of Viruses and Viroids Enabled by Use of Available mRNA and sRNA-Seq Datasets in Grapevine (Vitis vinifera L.)

2020

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Next-generation sequencing (NGS) based virome analyses of mRNA and sRNA have recently become a routine approach for reliable detection of plant viruses and viroids. In the present study we identified the viral/viroidal spectrum of several Indian grapevine cultivars and reconstructed their whole genomes using the publically available mRNAome and sRNAome datasets. Twenty three viruses and viroids (including two variants of grapevine leafroll associated virus 4) were identified from two tissues (fruit peels and young leaves) of three cultivars among which nine unique grapevine viruses and viroids were identified for the first time in India. Irrespective of the assemblers and tissues used, the mRNA based approach identified more acellular pathogens than the sRNA based approach across cultivars. Further, the mRNAome was on par with the whole transcriptome in viral identification. Through de novo assembly of transcriptomes followed by mapping against reference genome, we reconstructed 19 complete/near complete genomes of identified viruses and viroids. The reconstructed viral genomes included four larger RNA genomes (>13 kb), a DNA genome (RG grapevine geminivirus A), a divergent genome (RG grapevine virus B) and a genome for which no reference is available (RG grapevine virus L). A large number of SNPs detected in this study ascertained the quasispecies nature of viruses. Detection of three recombination events and phylogenetic analyses using reconstructed genomes suggested the possible introduction of viruses and viroids into India from several continents through the planting material. The whole genome sequences generated in this study can serve as a resource for reliable indexing of grapevine viruses and viroids in quarantine stations and certification programs.

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Section: Snp Detection and Recombination Analyses In Reconstructed Gementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Robust Virome Profiling and Whole Genome Reconstruction of Viruses and Viroids Enabled by Use of Available mRNA and sRNA-Seq Datasets in Grapevine (Vitis vinifera L.)

2020

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“…Remarkably, our results showed that the incidence of GFkV was higher than that of GFLV. High incidence of GFkV was also observed in other growing areas such as Andalucía (Spain), Istria (Croatia), Iran and Chile [53,[57][58][59]. In some viticulture regions, GFkV was the most widespread as compared to GLRaV-3, GLRaV-1 and GFLV [60,61].…”

Section: Discussionmentioning

confidence: 99%

Recovering Ancient Grapevine Cultivars in the Balearic Islands: Sanitary Status Evaluation and Virus Elimination

Aou-ouad

Montero

Baraza

et al. 2022

Plants

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Recuperation and genetic diversity preservation of local cultivars have acquired a huge interest in viticulture areas worldwide. In the Balearic Islands, most of the old cultivars are only preserved in grapevine germplasm banks, and so far, the sanitary status of these local cultivars has remained unexplored. The aim of this study was to survey and detect the virus incidence of all conserved cultivars in the government Grapevine Germplasm Bank of the Balearic Islands and to promote the sanitary recovery of two important minor cultivars, Argamussa and Gorgollassa. Enzyme-linked immunosorbent assay (ELISA) screenings were performed on 315 vines of 33 local cultivars. It was shown that the local cultivars were highly infected with simple (39.7%) and mixed infections (52.1%) and only 8.25% of them were free from the viruses tested. Grapevine leafroll-associated virus 3 (GLRaV-3) infection was the most common (82%). Moreover, Grapevine fanleaf virus (GFLV) and Grapevine fleck virus (GFkV) were also present with considerable incidence (25.4% and 43.5%, respectively). In addition, two sanitation protocols were used: shoot tip culture (ST) and thermotherapy in combination with shoot tip culture (CT). Virus elimination using only ST was effective to obtain “healthy” vines of cvs. Argamussa and Gorgollassa. It is important to emphasize that the methods described in the current study were rapid and effective in eliminating both GLRaV-3 and GFLV, also in combination.

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“…GFkV는 지표식물인Vitis rupestris에 서 잎에 얼룩을 나타내는 것을 제외하곤 대부분의 Vitis 종 (species)에서 증상을 드러내지 않는 잠복 감염형태로 존재 하나 (Martelli et al 2002), 식물 생리학적 측면에서 (Bota et al 2014), 그리고, 대개 다른 포도 바이러스와 복합감염 되어 있 을 경우에 생산량과 과실품질 측면에 부정적인 영향을 끼친 다고 하였다 (Cretazzo et al 2010a;Komar et al 2007;Kovacs et al 2001). GFkV는 세계적으로 감염분포가 매우 넓으며, 그 감 염이 지속적으로 보고되고 있는 가운데 (Cretazzo et al 2010b;Fiore et al 2008;Kumar et al 2013;Poojari et al 2016), 우리나라 에서도 재배되고 있는 몇몇 품종에서 감염보고가 있었다 (Kim et al 2011;Jo et al 2017). 포도작물에서의 바이러스 제거기술은 감염된 기내외 식 물을 열처리(thermotherapy)한 후 경정배양(shoot or meristem tip culture)을 하거나 Valero et al 2003;Skiada et al 2009) Duran-Vila 1988;Yossef et al 2009) 전통적인 방법 이외에, 체 세포 배양(somatic embryogenesis) (Gribaudo et al 2006;Gambino et al 2009;Borroto-Fernandez et al 2009), 항 바이러스제(antiviral drugs)를 이용한 화학처리법(chemotherapy) (Panattoni et al 2007;Skiada et al 2013), 초저온 동결법(cryopreservation) (Wang et al 2003), 경정조직 파편 배양(in vitro fragmented shoot apex culture) (Barlass 1987)법 등이 이용되었다.…”

Section: 서 언unclassified

Elimination ofGrapevine fleck virusfrom infected grapevines ‘Kyoho’ through meristem-tip culture of dormant buds

et al. 2017

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Herein, we report the meristem-tip culture from dormant buds of grape 'Kyoho' single-infected with Grapevine fleck virus (GFkV), which is phloem-limited and transmitted by graft inoculation. We produced GFkV-free shoots without thermo-or chemotherapy using meristem-tip explants approximately 0.3 mm (73 explants) and 0.8 mm long (five explants) including shoot apical meristem, 2-5 leaf primordia, and 1-4 uncommitted primordia from dormant buds of the infected woody cuttings (stored at 4°C). Explants were cultured on Murashige and Skoog (MS) medium supplemented with 3% sucrose, 3.0 mg/L benzyladenine (BA) and 0.1 mg/L indole-3-butyric acid (IBA). After 16 weeks of culture, shoot (10-mm long) regeneration frequency achieved from 0.3-mm explants was 4.1% and that obtained from 0.8-mm explants was 40.0%. Virus-free efficiency (expressed as the percentage of RT-PCR negative shoots regenerated) from 0.3-and 0.8-mm explants was 100% and 50%, respectively. Following in vitro multiplication, RT-PCR assays revealed identical results to assays of the first regenerated shoots. Our new methodological approach could be applied for eliminating other viruses in grapevines, as well as for producing virus-free plants in many other deciduous tree species, including fruit trees. which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Evidence of Grapevine leafroll associated virus-1–3, Grapevine fleck virus and Grapevine virus B Occurring in Himachal Pradesh, India

Cited by 16 publications

References 14 publications

Robust Virome Profiling and Whole Genome Reconstruction of Viruses and Viroids Enabled by Use of Available mRNA and sRNA-Seq Datasets in Grapevine (Vitis vinifera L.)

Robust Virome Profiling and Whole Genome Reconstruction of Viruses and Viroids Enabled by Use of Available mRNA and sRNA-Seq Datasets in Grapevine (Vitis vinifera L.)

Recovering Ancient Grapevine Cultivars in the Balearic Islands: Sanitary Status Evaluation and Virus Elimination

Elimination ofGrapevine fleck virusfrom infected grapevines ‘Kyoho’ through meristem-tip culture of dormant buds

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Evidence of Grapevine leafroll associated virus-1–3, Grapevine fleck virus and Grapevine virus B Occurring in Himachal Pradesh, India

Cited by 16 publications

References 14 publications

Robust Virome Profiling and Whole Genome Reconstruction of Viruses and Viroids Enabled by Use of Available mRNA and sRNA-Seq Datasets in Grapevine (Vitis vinifera L.)

Robust Virome Profiling and Whole Genome Reconstruction of Viruses and Viroids Enabled by Use of Available mRNA and sRNA-Seq Datasets in Grapevine (Vitis vinifera L.)

Recovering Ancient Grapevine Cultivars in the Balearic Islands: Sanitary Status Evaluation and Virus Elimination

Elimination ofGrapevine fleck virusfrom infected grapevines &lsquo;Kyoho&rsquo; through meristem-tip culture of dormant buds

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Elimination ofGrapevine fleck virusfrom infected grapevines ‘Kyoho’ through meristem-tip culture of dormant buds