Evidence of Free-Radical-Induced Damage in Rabbit Kidneys After Simple Hypothermic Preservation and Autotransplantation

Green, CJ; Healing, Guy; Lunec, J; Bj, Fuller; Simpkin, S.

doi:10.1097/00007890-198602000-00005

Cited by 76 publications

(17 citation statements)

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“…These results support the hypothesis that short-term caloric restriction improves the resistance to peroxidative damage by increasing the enzymatic capacity of decomposition of hydroperoxides [25]. It was demonstrated that the hypothermic preservation of organs resulted in increased contents of lipid peroxidation products [5]. An imbalance between the generation of free radicals and their removal was assumed since lipid peroxides could be reduced by the addition of scavenging agents to the preservation solutions [5,26,27].…”

Section: Discussionsupporting

confidence: 80%

“…It was demonstrated that the hypothermic preservation of organs resulted in increased contents of lipid peroxidation products [5]. An imbalance between the generation of free radicals and their removal was assumed since lipid peroxides could be reduced by the addition of scavenging agents to the preservation solutions [5,26,27]. Oxygen-free radicals are believed to produce renal dysfunction after renal ischemia and reperfusion as a result of altered antioxidant mechanisms [6,27].…”

Section: Discussionmentioning

confidence: 99%

“…Variations in antioxidant systems of various organs have been postulated as causes of different vulnerability of organs to drug-induced toxicity and dysfunction [5,6] . It has been suggested that lipid peroxidation is involved in pathologic processes during normothermic ischemia and reperfusion and is therefore one determinant responsible for successful clinical transplantation [5] . As organ donors often sustain periods of hyponutrition, the antioxidative status of the harvested organs might be affected [7].…”

mentioning

confidence: 99%

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Effect of Starvation on Antioxidant Enzymes and Respiratory Mitochondrial Functions in Kidney and Liver from Rats.

Jung

Henke

1997

J. Clin. Biochem. Nutr.

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SummaryThe influence of the nutritional status on the antioxidant defence system and on the mitochondrial respiratory functions of rat kidney and liver was studied. For that purpose, the antioxidant enzymes catalase, glutathione peroxidase, glutathione reductase, glutathione Stransferase, superoxide dismutase, the total glutathione and the respiration rates of states 3 and 4, the respiratory control ratio, and ADP/O ratio were measured before and after 2 and 4 days of starvation. The behaviour of the enzymes was not uniform. Only the liver glutathione peroxidase decreased by about 18% at second day of starvation, whereas all other enzymes in liver and kidney increased or remained unchanged. All mitochondrial parameters studied remained unaffected by starvation except the moderately reduced state 3 respiration and respiratory control ratio in liver mitochondria. We conclude from our experimental data that the enzymatic antioxidative status is of minor importance for the assumed vulnerability of kidney and liver after short-term starvation.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Effect of Starvation on Antioxidant Enzymes and Respiratory Mitochondrial Functions in Kidney and Liver from Rats.

Jung

Henke

1997

J. Clin. Biochem. Nutr.

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“…Since then many changes in the composition of solutions have been performed. Aiming at prolonging renal graft preservation time without, however, altering the organ's viability and function, studies have been performed by adding different substances to the perfusion solution such as oxygen 3 , steroids 4 , free radical scavengers 5,4 and calcium channel blockers such as trifluoperazine 6 , verapamil 7 and diltiazem 8,9 .…”

Section: Introductionmentioning

confidence: 99%

Histologic alterations of rat kidneys perfused with a Euro-Collins diltiazem solution

et al. 2010

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Purpose: Analyse the histologic changes of rat kidneys perfused with isotonic saline solution (ISS), Euro-Collins solution (ECS) and Euro-Collins solution with diltiazem (ECSD). Methods: Thirty-six Wistar rats were used divided equally, as follow: group A (ISS), group B (ECS) and group C (ECSD). Through a catheter placed into the abdominal aorta, a renal perfusion was performed using a solution according to the group to which the animal belonged. After the complete perfusion, bilateral nephrectomy was performed and the organs were preserved under hypothermia for five distinct periods of time. Glomerulus and tubule were evaluated through optical microscopy. Results: Renal perfusion with ECS and ECSD proved effectiveness in the preservation of the organs up to 36 hours and an increase in the percentage of injured glomeruli was noticed only in the period of 48 hours. Conclusions: The results showed that exists an association between the tubular injury and the glomeruli lesion degree; kidneys with a higher degree of tubular damage were related to severe glomerular lesion. Also, the addition of a calcium channel blocker, diltiazem, to the ECS for the renal perfusion does not decrease the percentage of glomerular lesion. Key words: Diltiazem. Perfusion. Kidney. Rats. RESUMOObjetivo: Analisar as alterações histológicas nos rins de ratos perfundidos com solução salina isotônica (ISS), solução Euro-Collins (ECS) e solução Euro-Collins com diltiazem (ECSD). Métodos: Foram divididos, de forma igual, 36 ratos Wistar, como se segue: grupo A (ISS), grupo B (ECS), grupo C (ECSD). Através de um cateter localizado na aorta abdominal, foi realizada a perfusão renal com a solução de acordo com o grupo ao qual o animal pertencia. Após a perfusão total, realizou-se nefrectomia bilateral com a preservação dos órgãos sob hipotermia por cinco períodos distintos de tempo. Glomérulos e túbulos foram avaliados por microscopia óptica. Resultados: Tanto a perfusão renal com ECS quanto a com ECSD provaram sua efetividade na preservação dos órgãos em até 36 horas e aumento da porcentagem de glomérulos injuriados foi notada apenas no período de 48 horas. Conclusões: Os resultados mostraram haver uma correlação entre a injúria tubular e o grau de lesão glomerular; rins com um maior grau de dano tubular foram relacionados com lesão glomerular severa. Além disso, a adição de um bloqueador de canal de cálcio, diltiazem, à ECS para a perfusão renal não diminui a porcentagem de lesão glomerular. Descritores: Diltiazem. Perfusão. Rim. Ratos.

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“…One proposed hypothesis of injury during hypothermia and freezing is the formation of oxygen-free radicals [5][6][7][8]. Whitley et al [9] have shown a rise in MDA levels of rat liver homogenates stored at high subzero temperatures.…”

Section: Introductionmentioning

confidence: 99%

Bone Marrow Cryopreservation: Improved Recovery Due to Bioantioxidant Additives in the Freezing Solution

Limaye

1997

STEM CELLS

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One hypothesis of cryoinjury is the damaging effect of oxygen-free radicals formed during freezing and thawing. Addition of physiologically acceptable antioxidants into the preservation solution improved the cryoprotection of bone marrow cells. Bone marrow nucleated cells were frozen using rate-controlled freezing devices. Antioxidants used in combination with 10% dimethylsulfoxide were α tocopheryl acetate, catalase, ascorbic acid, superoxide dismutase and reduced glutathione. The parameters used to assess the efficacy of cryopreservation were viability, nucleated cell recovery, and colony-forming unit assays: granulocytemacrophage and granulocyte-erythroid-macrophagemegakaryocyte. Results obtained indicate that the first three antioxidants increase the post-thaw recovery of cells, particularly in terms of early and late progenitors. Superoxide dismutase and reduced glutathione, however, have no beneficial effect on the preservation. The response of cryopreserved cells to suboptimal concentrations of colony-stimulating factors in in vitro assays was also restored to some extent when the cells were frozen with antioxidants.

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Evidence of Free-Radical-Induced Damage in Rabbit Kidneys After Simple Hypothermic Preservation and Autotransplantation

Cited by 76 publications

References 0 publications

Effect of Starvation on Antioxidant Enzymes and Respiratory Mitochondrial Functions in Kidney and Liver from Rats.

Effect of Starvation on Antioxidant Enzymes and Respiratory Mitochondrial Functions in Kidney and Liver from Rats.

Histologic alterations of rat kidneys perfused with a Euro-Collins diltiazem solution

Bone Marrow Cryopreservation: Improved Recovery Due to Bioantioxidant Additives in the Freezing Solution

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