Evidence of far ultraviolet light-mediated changes in plasma membrane structure and function

Braun, Andrew G.; Buckner, Christine A.

doi:10.1016/0005-2736(81)90042-0

Cited by 7 publications

(2 citation statements)

References 9 publications

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“…Flower petals which had been irradiated had a much decreased capacity, to take up sucrose (approximately 50%o, Table I). These results suggest that UV irradiation directly affects cell membranes and are in accordance with results reported for UV effects on lipid fluidity of membranes from mouse tumor cells (4) and on K+-ATPase activity of wheat root membranes (16).…”

supporting

confidence: 91%

Comparison between Ultraviolet Irradiation and Ethylene Effects on Senescence Parameters in Carnation Flowers

Borochov¹,

Faragher²

1983

Plant Physiol.

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The effects of ethylene aad ultraviolet (UV) (6,12). Several cell properties related to membranes have been reported to change with senescence. These include loss ofseveral membrane components (1, 2, 14), loss of semipermeability (12, 14), and a decrease in membrane lipid fluidity (3). Ethylene has been demonstrated to be directly involved in the regulation of flower senescence. Ethylene production increases sharply with senescence while exogenous application of ethylene enhanced flower senescence and wilting (6), increased permeability of petal cells (9, 14), and accelerated the decrease in cell membrane fluidity (15).Far-UV irradiation has been demonstrated to have several effects on plant cells. These include increase in cell membrane permeability (13), decrease in solute uptake capacity (5) raises the possibility of a common mechanism of action. The site of ethylene action is unknown, while that of UV irradiation might be more clearly identified physically. Therefore, a detailed comparison between the effects of these two agents might lead to a better understanding of senescence processes. MATERIALS AND METHODS Plant Material. Carnation flowers (Dianthus caryophyllus L. cvWhite Sim) were grown, harvested at the fully open stage, and prepared as outlined previously (11). Treatments with UV irradiation and ethylene were given 2 h after harvest and for the periods specified. Treatments and evaluations were carried out at 21°C, RH 75 ± 5%, and unless otherwise specified, at light intensity of Chemical Treatments. Ethylene was applied to the flowers at 10 ,4/l for 6 h as described previously (11). Silver thiosulfate was applied as a pulse treatment through the stems for 10 min at 4 mm as described by Reid et al. (10). Petal In-Rolling. Wilting of the flower petals was monitored by measuring the in-rolling of the petal tips. This was measured as the angle through which the petal tip had rolled, relative to the plane of the petal base.Petal Eletrolyte Leakage. The leakage of electrolytes from the petals into a 0.5 M mannitol bathing solution was measured essentially as described by Suttle and Kende (14). Ten petals were washed for 10 min and placed into 10 ml bathing solution for 10 h at 30°C. The conductivity ofthe resulting solution was measured using a Radiometer Copenhagen CDM 2e conductivity meter.Ethylene Measurements. Ethylene production by whole flowers was measured as described previously (11). Ethylene production of isolated petals was measured by sealing them in quartz cuvettes of 3.5 ml volume from which samples were taken at 15-intervals for ethylene determinations. After each sampling, the cuvettes were aerated with ethylene-free air.Sucrose Uptake.

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confidence: 91%

Comparison between Ultraviolet Irradiation and Ethylene Effects on Senescence Parameters in Carnation Flowers

Borochov¹,

Faragher²

1983

Plant Physiol.

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“…The hydrocarbon chain order of SM increased with mild oxidation and decreased with strong oxidation [167]. Other studies have reported that lipid oxidation orders the hydrophobic chains [148,[168][169][170][171][172][173][174][175][176]. It is not clear if the increased order of hydrophobic chains due to the lipid oxidation correlates with the binding of α-crystallin to the membrane and cataract formation.…”

Section: Lipid Peroxidation and Cataract Formationmentioning

confidence: 96%

Association of Alpha-Crystallin with Fiber Cell Plasma Membrane of the Eye Lens Accompanied by Light Scattering and Cataract Formation

Timsina

Mainali

2021

Membranes

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α-crystallin is a major protein found in the mammalian eye lens that works as a molecular chaperone by preventing the aggregation of proteins and providing tolerance to stress in the eye lens. These functions of α-crystallin are significant for maintaining lens transparency. However, with age and cataract formation, the concentration of α-crystallin in the eye lens cytoplasm decreases with a corresponding increase in the membrane-bound α-crystallin, accompanied by increased light scattering. The purpose of this review is to summarize previous and recent findings of the role of the: 1) lens membrane components, i.e., the major phospholipids (PLs) and sphingolipids, cholesterol (Chol), cholesterol bilayer domains (CBDs), and the integral membrane proteins aquaporin-0 (AQP0; formally MIP26) and connexins, and 2) α-crystallin mutations and post-translational modifications (PTMs) in the association of α-crystallin to the eye lens’s fiber cell plasma membrane, providing thorough insights into a molecular basis of such an association. Furthermore, this review highlights the current knowledge and need for further studies to understand the fundamental molecular processes involved in the association of α-crystallin to the lens membrane, potentially leading to new avenues for preventing cataract formation and progression.

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Leukotriene Synthesis by UV-Irradiated Macrophage Cell Cultures

Hardcastle¹,

Minoui²

1987

Prostaglandin and Lipid Metabolism in Radiation Injury

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Evidence of far ultraviolet light-mediated changes in plasma membrane structure and function

Cited by 7 publications

References 9 publications

Comparison between Ultraviolet Irradiation and Ethylene Effects on Senescence Parameters in Carnation Flowers

Comparison between Ultraviolet Irradiation and Ethylene Effects on Senescence Parameters in Carnation Flowers

Association of Alpha-Crystallin with Fiber Cell Plasma Membrane of the Eye Lens Accompanied by Light Scattering and Cataract Formation

Leukotriene Synthesis by UV-Irradiated Macrophage Cell Cultures

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