Evidence of Exposure to SARS-CoV-2 in Dogs and Cats from Households and Animal Shelters in Korea

Bae, Da-Yun; Tark, Dongseob; Moon, Sung-Hyun; Oem, Jae‐Ku; Kim, Won-Il; Park, Chul; Na, Ki-Jeong; Park, Choi‐Kyu; Oh, Yeonsu; Cho, Ho-Seong

doi:10.3390/ani12202786

Cited by 12 publications

(6 citation statements)

References 37 publications

(48 reference statements)

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“…They have also been found to shed SARS-CoV-2 viral particles through their respiratory tract and intestines (11,12). Transmission of SARS-CoV-2 among domestic cats has been reported (13), as well as transmission from human to cat (14). A series of reports from various countries has con rmed that pet owners are frequent sources of SARS-CoV-2 infection for their cats (8,(14)(15)(16)(17).…”

Section: Full Textmentioning

confidence: 99%

No detection of SARS-CoV-2 in domestic cats exposed to infected owners during the Omicron Wave in São Paulo, Brazil

Lopes

Rigato

Kasinski

et al. 2023

Preprint

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been demonstrated to infect various mammals, including cats, dogs, and minks. Given the close coexistence of humans and cats, transmission of SARS-CoV-2 variants between these two species has been documented. This study involved the sampling of eight domestic cats that resided solely with their infected owners in Brazil, during the early stage of the Omicron variant wave. Nasal and anal secretion samples obtained from the eight cats were subjected to RT-qPCR analysis, which revealed no detection of SARS-CoV-2. Additionally, serum samples collected from these cats did not show the presence of anti-SARS-CoV-2 antibodies. The findings of this study indicate that the Omicron variant has a low potential to infect felines, which contributes to the absence of transmission. Therefore, cats do not appear to play a significant role in the transmission chain of SARS-CoV-2.

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Section: Full Textmentioning

confidence: 99%

No detection of SARS-CoV-2 in domestic cats exposed to infected owners during the Omicron Wave in São Paulo, Brazil

Lopes

Rigato

Kasinski

et al. 2023

Preprint

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“…The PCR mixture was composed of a 10 µL solution, which included 3 µL of a master mix consisting of polymerase, reverse transcriptase, buffer, and stabilizer, 1 µL of the primer/probe set, 1 µL of an internal positive control, and 5 µL of the extracted RNA, setting up the reaction. The thermal cycling parameters were set to reverse transcription at 50 • C for 10 min, initial denaturation at 95 • C for 3 min, followed by 45 cycles of denaturation at 95 • C for 9 s and annealing/extending at 58 • C for 30 s. A cycle threshold (Ct) value below 40 was indicative of a positive result for the detection of each targeted gene [13].…”

Section: Extraction Of Nucleic Acids and Real-time Qpcr For Reverse T...mentioning

confidence: 99%

“…Grasping the role of domestic livestock, alongside the recognized significance of pets in SARS-CoV-2 transmission, is vital for safeguarding both animal and human health. Advancements in this area will contribute to pinpointing domestic reservoirs and forecasting the virus's potential for endemic persistence [13]. Research indicates that dogs and cats can contract SARS-CoV-2; however, the susceptibility of farm animals to this particular virus and its prevalence in these populations under natural conditions remain underexplored [1].…”

Section: Introductionmentioning

confidence: 99%

“…The prevalence of infection among companion animals like dogs and cats has been extensively studied in various countries, including Italy, China, the United States, and Korea [13][14][15][16][17]. However, when it comes to the native animals of each country, specifically indigenous livestock, there is a lack of research on their infection status.…”

Section: Introductionmentioning

confidence: 99%

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Demonstration of SARS-CoV-2 Exposure in Korean Native Cattle and Korean Native Black Goats in Korea

Bae,

Yang,

Moon

et al. 2023

Animals

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The COVID-19 pandemic is caused by the zoonotic SARS-CoV-2 virus. A wide range of animals that interact with humans have been investigated to identify potential infections. As the extent of infection became more apparent, extensive animal monitoring became necessary to assess their susceptibility. This study analyzed nasal swabs and blood samples collected from randomly selected Korean native cattle and Korean native black goats. The tests conducted included real-time qPCR to detect SARS-CoV-2 antigens, an ELISA to detect antibodies, and a plaque reduction neutralization test (PRNT) to determine the presence of neutralizing antibodies. Among the 1798 animals tested (consisting of 1174 Korean native cattle and 624 Korean native black goats), SARS-CoV-2 viral RNA was detected in one Korean native cattle and one Korean native black goat. ELISA testing revealed positive results for antibodies in 54 Korean native cattle (4.60%) and 16 Korean native black goats (2.56%), while PRNTs yielded positive results in 51 Korean native cattle (4.34%) and 14 Korean native black goats (2.24%). The presence of SARS-CoV-2 antigens and/or antibodies was identified in animals on farms where farmworkers were already infected. It is challenging to completely rule out the possibility of reverse zoonotic transmission from humans to livestock in Korea, although the transmission is not to the same extent as it is in highly susceptible animal species like minks, cats, and dogs. This is due to the limited geographical area and the dense, intensive farming practices implemented in these regions. In conclusion, continuous viral circulation between humans and animals is inevitable, necessitating ongoing animal monitoring to ensure public health and safety.

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“…Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is one of the novel CoVs and is the etiological agent of the global COVID‐19 pandemic. The COVID‐19 pandemic is currently driven by human‐to‐human transmission; however, many spillover events from infected humans to companion animals have been reported (Bae et al., 2022 ; Fuentealba et al., 2021 ; Jairak et al., 2022 ; Jones et al., 2023 ; Pecora et al., 2022 ). Cats are the most frequently kept companion animal species in Europe (FEDIAF, 2020 ) and an increasing number of cats are kept in close contact with humans and at the same time a considerable proportion of these animals roam freely, thus serving as a link between household and the environment.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of SARS‐CoV‐2 in domestic cats (Felis catus) during COVID‐19 pandemic in Latvia

Mūrniece,

Šteingolde,

Cvetkova

et al. 2023

Veterinary Medicine & Sci

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BackgroundThe causative agent of the COVID‐19 pandemic, severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), is of zoonotic origin and has shown reverse zoonotic transmissibility.ObjectivesThe aim of this cross‐sectional study was to investigate the serological and molecular prevalence of SARS‐CoV‐2 infection in the domestic cat (Felis catus) population from Latvia in natural conditions and subsequently perform viral genome analysis.MethodsOropharyngeal and rectal swabs and blood samples were collected from 273 domestic cats during the second wave of COVID‐19 infection in Latvia. Molecular prevalence was determined by using reverse transcriptase‐polymerase chain reaction (RT‐PCR). Serum samples were analysed via double antigen enzyme‐linked immunosorbent assay targeting the antibody against the nucleocapsid protein of SARS‐CoV‐2. Positive swab samples were analysed using whole viral genome sequencing and subsequent phylogenetic analysis of the whole genome sequencing data of the samples was performed.ResultsThe overall SARS‐CoV‐2 RT‐PCR positivity and seroprevalence was 1.1% (3/273) and 2.6% (7/273), respectively. The SARS‐CoV‐2 genome sequences from three RT‐PCR positive cats were assigned to the three common lineages (PANGOLIN lineage S.1.; B.1.177.60. and B.1.1.7.) circulating in Latvia during the particular period of time.ConclusionsThese findings indicate that feline infection with SARS‐CoV‐2 occurred during the second wave of the COVID‐19 pandemic in Latvia, yet the overall prevalence was low. In addition, it seems like no special ‘cat’ pre‐adaptations were necessary for successful infection of cats by the common lineages of SARS‐CoV‐2.

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Evidence of Exposure to SARS-CoV-2 in Dogs and Cats from Households and Animal Shelters in Korea

Cited by 12 publications

References 37 publications

No detection of SARS-CoV-2 in domestic cats exposed to infected owners during the Omicron Wave in São Paulo, Brazil

No detection of SARS-CoV-2 in domestic cats exposed to infected owners during the Omicron Wave in São Paulo, Brazil

Demonstration of SARS-CoV-2 Exposure in Korean Native Cattle and Korean Native Black Goats in Korea

Prevalence of SARS‐CoV‐2 in domestic cats (Felis catus) during COVID‐19 pandemic in Latvia

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