Evidence of cytosolic iron release during post‐ischaemic reperfusion of isolated rat hearts Influence on spin‐trapping experiments with DMPO

Boucher, François; Pucheu, Sylvie; Coudray, Charles; Favier, A.; Leiris, Joël de

doi:10.1016/0014-5793(92)80455-p

Cited by 30 publications

(7 citation statements)

References 21 publications

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“…Release and redistribution of redox-active iron during IR in the liver as well as other organs has been observed by several research laboratories [162][163][164][165][166][167]. Increased levels of mobilized iron indicate a state of increased sensitivity for the cells and may represent the connecting link between H 2 O 2 production and tissue damage during reperfusion.…”

Section: Redox-active Iron In Hepatic Ir Injurymentioning

confidence: 95%

Oxidative Stress in Hepatic Ischemia-Reperfusion Injury: The Role of Antioxidants and Iron Chelating Compounds

Galaris¹,

Barbouti²,

Korantzopoulos

2006

CPD

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Ischemia-reperfusion (IR) injury is a multifactorial process triggered when the liver or other organs are transiently subjected to reduced blood supply followed by reperfusion. It has been shown that "reactive oxygen species" (ROS) are generated during ischemia and reperfusion and may represent pivotal mediators of the ensuing pathological complications. In some cases, however, moderate production of ROS may exert protective effects, a phenomenon presumably related to "ischemic preconditioning". This review will focus mainly on: a) describing the sources and the biochemical mechanisms of ROS generation during ischemia and reperfusion, b) discussing current developments in understanding the biochemical pathways by which ROS may induce toxic or protective effects, c) critically evaluating the results of previous attempts to counteract the toxic effects of ROS by using a variety of antioxidant and transition metalchelating agents, and d) if feasible, proposing potential new pharmaceutical agents aimed at ameliorating ROS-inducing deleterious effects during reperfusion. It is concluded that ROS are generated from different sources, at different periods during IR, and may act by a variety of not well understood biochemical mechanisms which ultimately lead to cell damage and tissue failure.

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Section: Redox-active Iron In Hepatic Ir Injurymentioning

confidence: 95%

Oxidative Stress in Hepatic Ischemia-Reperfusion Injury: The Role of Antioxidants and Iron Chelating Compounds

Galaris¹,

Barbouti²,

Korantzopoulos

2006

CPD

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“…Among several nitrones used as spin traps, 5,5-dimethyl-1-pyrroline N-oxide (DMPO) has received the most attention. Reaction of this spin trap with superoxide and hydroxyl radicals or radicals of a lipid nature derived from hydroxyl radicals produces spin-trapped adducts with characteristic EPR spectra (5,6). Moreover, spin trapping using DMPO has been used to detect and distinguish between carbon-centered, alkoxyl, and peroxyl radicals produced during metal ion-and lipoxygenase-catalyzed breakdown (7), as well as photolytic breakdown of peroxidized fatty acids (8).…”

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confidence: 99%

Virgin olive oil: Free radical production studied with spin‐trapping electron paramagnetic resonance spectroscopy

Skoutas

Haralabopoulos

Avramiotis

et al. 2001

J Americ Oil Chem Soc

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Spin trapping using 5,5-dimethyl-1-pyrroline Noxide (DMPO) has been used to detect and distinguish free radicals in samples of Greek extra virgin olive oils. A number of the samples examined immediately after the addition of the spin trap showed a spontaneous complex electron paramagnetic resonance (EPR) signal. The majority of DMPO-radical adducts formed (80-90%) represented peroxyl and alkoxyl radical adducts. Similar spectra were recorded when DMPO was added in oxidized triolein and then treated with Fe 2+ , Fe 3+ , or Cu 2+ or when EPR-silent olive oil samples were treated with these metallic ions. Metal ion-catalyzed decomposition of triolein hydroperoxides, as recorded by EPR signal intensity, increased with increasing metal ion concentration in the micromolar range. The relative concentration of alkoxyl-DMPO adducts increased with increasing Fe 2+ or Fe 3+ concentration, whereas that of peroxyl-DMPO species decreased. In contrast, the relative concentrations of alkoxyl and peroxyl species produced by Cu 2+ were similar over the whole metal concentration range examined. Exposure of EPR-silent virgin olive oil or oxidized triolein to ultraviolet light in the presence of DMPO resulted in the detection of a three-line spectrum characterized by wide line widths.Paper no. J9852 in JAOCS 78, 1121-1125 (November 2001).KEY WORDS: Copper, 5,5-dimethyl-1-pyrroline N-oxide (DMPO), electron paramagnetic resonance spectroscopy (EPR), free radicals, iron, olive oil, spin trapping.It has long been known that oxidative rancidity is the main deteriorative change of olive oil during storage and that it is due to the oxidation of unsaturated fatty acids and the subsequent formation of compounds possessing an unpleasant taste and odor (1). This general oxidation process affecting the stability of vegetable oils is often called autoxidation and involves a free radical mechanism. It is assumed that hydroperoxide groups attach to the carbon atom of unsaturated fatty compounds, and subsequently, the breakdown of hydroperoxides gives a chain reaction of autoxidation (2,3). Olive oil oxidation is affected by a number of factors, such as oxygen, temperature, presence of metals and chromophores, light, and ionizing radiation, whereas the resistance of virgin olive oil to oxidation is related to the high levels of monounsaturated triacylglycerols and the presence of natural antioxidants (1). Transition metals, especially iron and copper, are known contaminants of virgin olive oil and may act as pro-oxidant factors because they catalyze both the generation of free radicals and the decomposition of hydroperoxides (1-3). The oxidation of unsaturated fats is also accelerated by exposure to light, and direct photo-oxidation is due to free radicals produced by ultraviolet (UV) irradiation, which catalyzes the decomposition of hydroperoxides and other oxygen complexes of unsaturated lipids (2,3).Of the available methods for the detection of free radicals, only spin trapping offers the opportunity to simultaneously measure and distinguish amon...

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“…Iron metabolism is normally thought to be tightly controlled ; nevertheless, the chemical nature and subcellular distribution of free iron within cells are completely unknown, except that this pool seems to be normally kept very small (Crichton and Charloteaux-Wauters, 1987 ;Halliwell, 1992) . In addition, certain pathological conditions are known to increase free iron concentrations by releasing this ion from ferritin (Thomas and Aust, 1986;Boucher et al, 1992) . It should be noted that even the lowest concentration of iron (11) or (111) (30 pM) used in our studies was found to increase significantly the production of TOPA compounds over baseline autoxidation .…”

Section: Discussionmentioning

confidence: 99%

Iron‐Mediated Oxidation of 3,4‐Dihydroxyphenylalanine to an Excitotoxin

Newcomer

Rosenberg

Aizenman

1995

Journal of Neurochemistry

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2,4,5‐Trihydroxyphenylalanine (TOPA) oxidizes in solution to form a quinone derivative that is a non‐NMDA glutamatergic agonist and neurotoxin. DOPA can autoxidize in physiological solutions to form small amounts of both TOPA and TOPA quinone. We report here that this conversion can be dramatically enhanced by iron (II) alone, but more so by iron (II) in the presence of hydrogen peroxide. This conversion is of sufficient magnitude that the resulting product can elicit non‐NMDA, glutamate receptor‐mediated electrical responses in cultured cortical neurons isolated from rat. This finding suggests that TOPA quinone may play a role in pathological processes involving abnormal iron metabolism in catecholaminergic neurons.

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Evidence of cytosolic iron release during post‐ischaemic reperfusion of isolated rat hearts Influence on spin‐trapping experiments with DMPO

Cited by 30 publications

References 21 publications

Oxidative Stress in Hepatic Ischemia-Reperfusion Injury: The Role of Antioxidants and Iron Chelating Compounds

Oxidative Stress in Hepatic Ischemia-Reperfusion Injury: The Role of Antioxidants and Iron Chelating Compounds

Virgin olive oil: Free radical production studied with spin‐trapping electron paramagnetic resonance spectroscopy

Iron‐Mediated Oxidation of 3,4‐Dihydroxyphenylalanine to an Excitotoxin

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