Evidence of circulation of an epidemic strain of Pasteurella multocida in Jiangsu, China by multi-locus sequence typing (MLST)

Wang, Yanhong; Jun, Zhu; Chen, Lu; Wu, Bozeng; Liu, Dandan; Wei, Hang; Liu, Huimou; Liu, Xiufan

doi:10.1016/j.meegid.2013.07.027

Cited by 13 publications

(12 citation statements)

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“…Pasteurellosis is one of the most common diseases of some wild and domestic animals worldwide. The cases of P. multocida infected animals have been widely reported in recent years in China [ 38 , 43 ]. However, the distribution and prevalence of serotypes and pathotypes of P. multocida isolates may vary considerably from different regions and times.…”

Section: Discussionmentioning

confidence: 99%

“…Fowl cholera is an acute, fatal septicemic disease of various domestic and wild bird species, which are responsible for significant economic loss in poultry industries around the world [ 18 , 28 , 32 ]. Pasteurella multocida ( P. multocida ) has been reported to be the cause of fowl cholera outbreaks [ 43 ]. P. multocida can also lead to atrophic rhinitis in swine, hemorrhagic septicemia in bovine and buffaloes, fowl cholera, snuffles in rabbits and infected finger bone in humans [ 44 ].…”

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confidence: 99%

“…Gene technologies such as 16S rRNA , restriction endonuclease analysis (REA), ribotyping, random amplification of polymorphic DNA (RAPD)-PCR, pulsed-field gel electrophoresis (PFGE) and multilocus sequence type analysis (MLST), have pushed forward studies of molecular epidemiology and genetic diversity of P. multocida [ 24 , 35 , 43 ]. MLST is considered the appropriate bacterial typing techniques for global and long-term studies [ 13 , 36 ] and the results can be easily compared among different laboratories for long-term and global surveillance of bacterial strains [ 36 , 43 ]. MLST is effective in identifying cases or outbreaks related to P. multocida infection, as well as identifying the source and dissemination events [ 33 ].…”

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confidence: 99%

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Investigation of genetic diversity and epidemiological characteristics of <i>Pasteurella multocida</i> isolates from poultry in southwest China by population structure, multi-locus sequence typing and virulence-associated gene profile analysis

Cheng

Lan

et al. 2018

The Journal of Veterinary Medical Science

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Fowl cholera caused by Pasteurella multocida has always been a disease of global importance for poultry production. The aim of this study was to obtain more information about the epidemiology of avian P. multocida infection in southwest China and the genetic characteristics of clinical isolates. P. multocida isolates were characterized by biochemical and molecular-biological methods. The distributions of the capsular serogroups, the phenotypic antimicrobial resistance profiles, lipopolysaccharide (LPS) genotyping and the presence of 19 virulence genes were investigated in 45 isolates of P. multocida that were associated with clinical disease in poultry. The genetic diversity of P. multocida strains was performed by 16S rRNA and rpoB gene sequence analysis as well as multilocus sequence typing (MLST). The results showed that most (80.0%) of the P. multocida isolates in this study represented special P. multocida subspecies, and 71.1% of the isolates showed multiple-drug resistance. 45 isolates belonged to capsular types: A (100%) and two LPS genotypes: L1 (95.6%) and L3 (4.4%). MLST revealed two new alleles (pmi77 and gdh57) and one new sequence type (ST342). ST129 types dominated in 45 P. multocida isolates. Isolates belonging to ST129 were with the genes ompH+plpB+ptfA+tonB, whereas ST342 included isolates with fur+hgbA+tonB genes. Population genetic analysis and the MLST results revealed that at least one new ST genotype was present in the avian P. multocida in China. These findings provide novel insights into the epidemiological characteristics of avian P. multocida isolates in southwest China.

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Section: Discussionmentioning

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Investigation of genetic diversity and epidemiological characteristics of <i>Pasteurella multocida</i> isolates from poultry in southwest China by population structure, multi-locus sequence typing and virulence-associated gene profile analysis

Cheng

Lan

et al. 2018

The Journal of Veterinary Medical Science

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“…Phylogenetic analyses were conducted on the basis of the concatenation of seven MLST loci ( purA, fumC, icd, mdh, recA, gyrB and adk ) using the MEGA v4.1 software as previously described [31]. The result is presented as a neighbour-joining tree; the robustness of the groupings was assessed by bootstrap resampling of 1,000 replicates.…”

Section: Methodsmentioning

confidence: 99%

Characterization and virulence clustering analysis of extraintestinal pathogenic Escherichia coli isolated from swine in China

Zhu

Wang

et al. 2017

BMC Vet Res

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BackgroundSwine extraintestinal pathogenic Escherichia coli (ExPEC) is an important pathogen that leads to economic and welfare costs in the swine industry worldwide, and is occurring with increasing frequency in China. By far, various virulence factors have been recognized in ExPEC. Here, we investigated the virulence genotypes and clonal structure of collected strains to improve the knowledge of phylogenetic traits of porcine ExPECs in China.ResultsWe isolated 64 Chinese porcine ExPEC strains from 2013 to 14 in China. By multiplex PCR, the distribution of isolates belonging to phylogenetic groups B1, B2, A and D was 9.4%, 10.9%, 57.8% and 21.9%, respectively. Nineteen virulence-related genes were detected by PCR assay; ompA, fimH, vat, traT and iutA were highly prevalent. Virulence-related genes were remarkably more prevalent in group B2 than in groups A, B1 and D; notably, usp, cnf1, hlyD, papA and ibeA were only found in group B2 strains. Genotyping analysis was performed and four clusters of strains (named I to IV) were identified. Cluster IV contained all isolates from group B2 and Cluster IV isolates had the strongest pathogenicity in a mouse infection model. As phylogenetic group B2 and D ExPEC isolates are generally considered virulent, multilocus sequence typing (MLST) analysis was performed for these isolates to further investigate genetic relationships. Two novel sequence types, ST5170 and ST5171, were discovered. Among the nine clonal complexes identified among our group B2 and D isolates, CC12 and CC95 have been indicated to have high zoonotic pathogenicity. The distinction between group B2 and non-B2 isolates in virulence and genotype accorded with MLST analysis.ConclusionThis study reveals significant genetic diversity among ExPEC isolates and helps us to better understand their pathogenesis. Importantly, our data suggest group B2 (Cluster IV) strains have the highest risk of causing animal disease and illustrate the correlation between genotype and virulence.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-017-0975-x) contains supplementary material, which is available to authorized users.

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“…ST 282 isolated from chicken is a SLV of ST 68 which has been isolated from Chimpanzee of Ivory Coast (Table 2). ST 129 isolated from chicken has already been isolated from fowl cholera cases in China (Wang et al, 2013) and from HS cases in Sri Lanka. This suggest that probably this strain has been transmitted from Sri Lanka and/or China to India or vice versa because of movement of animals/birds via border or trade in past.…”

Section: Discussionmentioning

confidence: 99%

Molecular Epidemiology ofPasteurella multocidaCirculating in India by Multilocus Sequence Typing

Sarangi

Thomas

Gupta

et al. 2014

Transbound Emerg Dis

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Multilocus sequence typing (MLST), a sequence-based typing method for bacterial pathogens, is currently the best method for long-term epidemiological study and to understand the population structure of the bacteria. This investigation was carried out to study the diversity of Pasteurella multocida isolates circulating in India. Ten different sequence types (ST) identified in this study are ST 122 from cattle, goat, mithun and pig; ST 50 from pig; ST 9 from cattle and sheep; ST 229 from cattle and goat; ST 71 and ST 277 from cattle; and ST 129, ST 280, ST 281 and ST 282 from avian species. Of these, ST 277, ST 280, ST 281 and ST 282 were identified for the first time. The analysis of results provides novel epidemiological information on the circulation of multiple STs across India. The majority of STs or their variants identified in this study have already been reported from different parts of the globe. This suggests that probably transboundary spread of strains across countries and continents has occurred across evolutionary time and is still happening. The isolation of ST 122 from small ruminants and pigs suggests that these species may be included in the preventive vaccination policy for effective control of haemorrhagic septicaemia in India.

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Evidence of circulation of an epidemic strain of Pasteurella multocida in Jiangsu, China by multi-locus sequence typing (MLST)

Cited by 13 publications

References 23 publications

Investigation of genetic diversity and epidemiological characteristics of <i>Pasteurella multocida</i> isolates from poultry in southwest China by population structure, multi-locus sequence typing and virulence-associated gene profile analysis

Investigation of genetic diversity and epidemiological characteristics of <i>Pasteurella multocida</i> isolates from poultry in southwest China by population structure, multi-locus sequence typing and virulence-associated gene profile analysis

Characterization and virulence clustering analysis of extraintestinal pathogenic Escherichia coli isolated from swine in China

Molecular Epidemiology ofPasteurella multocidaCirculating in India by Multilocus Sequence Typing

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