Evidence of Cholecystokinin Release by Bombesin in the Dog

Abstract

1The intravenous infusion of bombesin elicited in the dog a contraction of the gall bladder with decreased opening pressure of the choledocho-duodenal junction and stimulation of pancreatic secretion. 2 The pancreatic juice -produced under the influence of bombesin was poor in bicarbonate and rich in protein. Threshold doses of the peptide were of the order of 0.25 ,ug kg-' h-1 and maximum protein output was obtained with 1 pg kg'1 h-1. The pancreatic protein response to bombesin was very similar, in its onset and duration, to that elicited by intraduodenal infusion of L-tryptophan. Infusions of bombesin repeated at short intervals produced tachyphylaxis. 3 Antrectomy did not affect the stimulant action of bombesin on the pancreas. Atropine however, reduced the pancreatic protein response to bombesin. 4 It is suggested that bombesin acts on the gall bladder and the exocrine pancreas through release of cholecystokinin from the duodenal mucosa. No release of secretin could be demonstrated. It is likely that the releasing activity of bombesin is limited, in the field of gastrointestinal peptides, to those belonging to the gastrin-cholecystokinin family..

Gastrin Release by Bombesin in the Dog

“…However, it is also possible that at least the increase in gastric acid secretion is elicited by cholecystokinin released by bombesin. There is in fact strong evidence that bombesin besides releasing gastrin may also cause a release of cholecystokinin from the duodenal mucosa of the dog (Erspamer, Improta, Melchiorri & Sopranzi, 1974).…”

In vitro action of bombesin and bombesin-like peptides on amylase secretion, calcium efflux, and adenylate cyclase activity in the rat pancreas: a comparison with other secretagogues.

“…The Italian investigators have recently demonstrated that a perfusion of bombesin markedly stimulates the secretion of pancreatic juice rich in hydrolases in man (15), dog (16), and chicken (17). Erspamer et al (16) have suggested that the pancreatic response and the acceleration of gall bladder emptying (16,18) are secondary to the release of CCK-PZ from the duodenal mucosa.…”

In vitro action of bombesin on amylase secretion, membrane potential, and membrane resistance in rat and mouse pancreatic acinar cells. A comparison with other secretagogues.

“…The tetradecapeptide bombesin, that can be isolated from amphibian skin, is a powerful stimulant of pancreatic secretion in several species including man (1)(2)(3)(4). Bombesin-like peptides are present in the mamReceivedfor publication 11 April 1977 and in revised form 6 July 1977. malian gastrointestinal tract (5,6).…”