Evidence of bovine leukemia virus circulating in sheep and buffaloes in Colombia: insights into multispecies infection

Olaya-Galán, Nury Nathalia; Corredor-Figueroa, Adriana P.; Velandia-Álvarez, Sebastian; Vargas-Bermúdez, Diana S.; Fonseca-Ahumada, Nathalia; Nuñez, Kerlimber; Jaime, J.; Gutiérrez, María Lucía Lahoz

doi:10.1007/s00705-021-05285-7

Cited by 8 publications

(3 citation statements)

References 56 publications

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“…It causes enzootic bovine leukemia (EBL) mainly in dairy and beef cattle [ 44 ]. Recently, BLV was found to be in circulation in sheep in Colombia [ 45 ]. Infection affects B cells in cattle, impairs the immune system, reduces milk yield, shortens lifespan, and causes tumor formation in approximately 1–5% of animals [ 46 , 47 ].…”

Section: Ruminant Viral Diseases and Lamp Assays For Diagnosismentioning

confidence: 99%

Diagnosis of Ruminant Viral Diseases with Loop-Mediated Isothermal Amplification

2023

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Infectious diseases in livestock industry are major problems for animal health, food safety, and the economy. Zoonotic diseases from farm animals are significant threat to human population as well. These are notifiable diseases listed by the World Organization for Animal Health (OIE). Rapid diagnostic methods can help keep infectious diseases under control in herds. Loop-mediated isothermal amplification (LAMP) is a simple and rapid nucleic acid amplification method that is studied widely for detection of many infectious diseases in the field. LAMP allows biosensing of target DNA or RNA under isothermal conditions with high specificity in a short period of time. An untrained user can analyze results based on color change or turbidity. Here we review LAMP assays to diagnose OIE notifiable ruminant viral diseases in literature highlighting properties of LAMP method considering what is expected from an efficient, field usable diagnostic test.

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Section: Ruminant Viral Diseases and Lamp Assays For Diagnosismentioning

confidence: 99%

Diagnosis of Ruminant Viral Diseases with Loop-Mediated Isothermal Amplification

2023

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“…Even if cattle is the natural host of the virus, evidence of the presence of BLV has also been reported in other species (Lee et al, 2012;Barez et al, 2015;Olaya-Galán et al, 2021a). Thus, BLV has been described as a versatile agent that could infect multiple hosts both naturally and under controlled conditions in the laboratory (Gillet et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

In vitro Susceptibility of Human Cell Lines Infection by Bovine Leukemia Virus

et al. 2022

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Evidence of the presence of bovine leukemia virus (BLV) in human beings and its association with breast cancer has been published in the literature, proposing it as a zoonotic infection. However, not enough evidence exists about transmission pathways nor biological mechanisms in human beings. This study was aimed at gathering experimental evidence about susceptibility of human cell lines to BLV infection. Malignant and non-malignant human cell lines were co-cultured with BLV-infected FLK cells using a cell-to-cell model of infection. Infected human cell lines were harvested and cultured for 3 to 6 months to determine stability of infection. BLV detection was performed through liquid-phase PCR and visualized through in situ PCR. Seven out of nine cell lines were susceptible to BLV infection as determined by at least one positive liquid-phase PCR result in the 3-month culture period. iSLK and MCF7 cell lines were able to produce a stable infection throughout the 3-month period, with both cytoplasmic and/or nuclear BLV-DNA visualized by IS-PCR. Our results support experimental evidence of BLV infection in humans by demonstrating the susceptibility of human cells to BLV infection, supporting the hypothesis of a natural transmission from cattle to humans.

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“…Bovine leukemia virus (BLV) is a deltaretrovirus which is able to infect B-lymphocytes in cattle and induces proliferation in these cells, which subsequently leads to enzootic bovine leukosis (EBL) in cattle—a malignant neoplastic disease that mainly involve the lymph nodes, in the form of lymphoma or lymphosarcoma [ 2 ]. Since BLV has been shown to be transmitted to sheep, buffalos and other small ruminants, causing an EBL-like disease in these animals [ 3 , 4 , 5 ], and there are a lot of morphological similarities between EBL and adult camel leukosis, there has been a lot of interest in seeing whether this virus can also lead to adult camel leukosis in dromedaries. Although preliminary studies using PCR amplification for BLV on blood samples of apparently healthy dromedaries have not shown any positive results [ 6 , 7 ], there is still concern as to whether BLV may be associated with adult camel leukosis in dromedaries, as PCR amplification may not be able to pick up BLV or its genetic variants with significant differences from BLV, and the virus may be present in the tissue of dromedaries with adult camel leukosis instead of blood in healthy camels.…”

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confidence: 99%

Usefulness of Next-Generation Sequencing in Excluding Bovine Leukemia Virus as a Cause of Adult Camel Leukosis in Dromedaries

Wernery,

Teng,

et al. 2023

Pathogens

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Adult camel leukosis is an emerging hematological and neoplastic disease in dromedaries. It has been hypothesized that bovine leukemia virus (BLV) or its genetic variants may be associated with adult camel leukosis. In this study, we used next-generation sequencing (NGS) to detect all possible viruses in five lung samples from five dromedaries with histopathological evidence of adult camel leukosis and four tissue samples from two control dromedaries. A total throughput of 114.7 Gb was achieved, with an average of 12.7 Gb/sample. For each sample, all the pair-end 151-bp reads were filtered to remove rRNA sequences, bacterial genomes and redundant sequences, resulting in 1–7 Gb clean reads, of which <3% matched to viruses. The largest portion of these viral sequences was composed of bacterial phages. About 100–300 reads in each sample matched “multiple sclerosis-associated retrovirus”, but manual analysis showed that they were only repetitive sequences commonly present in mammalian genomes. All viral reads were also extracted for analysis, confirming that no BLV or its genetic variants or any other virus was detected in the nine tissue samples. NGS is not only useful for detecting microorganisms associated with infectious diseases, but also important for excluding an infective cause in scenarios where such a possibility is suspected.

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Evidence of bovine leukemia virus circulating in sheep and buffaloes in Colombia: insights into multispecies infection

Cited by 8 publications

References 56 publications

Diagnosis of Ruminant Viral Diseases with Loop-Mediated Isothermal Amplification

Diagnosis of Ruminant Viral Diseases with Loop-Mediated Isothermal Amplification

In vitro Susceptibility of Human Cell Lines Infection by Bovine Leukemia Virus

Usefulness of Next-Generation Sequencing in Excluding Bovine Leukemia Virus as a Cause of Adult Camel Leukosis in Dromedaries

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