Evidence of an anti‐A₁ inhibited by EDTA

Abstract

EDTA is added to some commercial A and B cells as a means of preventing hemolysis in serum testing. Unexpected results in serum testing have been reported with EDTA-dependent agglutinins, but not with agglutinins inhibited by EDTA. A serum sample was found to contain an anti-A1 undetected by serum testing, but resulting in incompatible crossmatches. Studies indicated that reactivity was inhibited by the EDTA present in the diluent of the A reagent red cells.

In vitro reactions with red blood cells that are not due to blood group antibodies: a review

“…28 Reports in the earlier literature, of calcium-and citrate-dependent agglutinins, may sometimes be explained by the same mechanism. [30][31][32] In 1997, Joshi 33 described a citratedependent autoantibody that caused an error in ABO grouping.Tedrow and Zeigler 34 described an anti-A 1 that was inhibited by EDTA (i.e., it did not react with the group A RBCs from the manufacturer that uses EDTA in its suspending medium). Green et al 35 described a patient with cold agglutinin syndrome associated with an IgM anti-Pr 1d .…”

Equivalence of spray-dried K2EDTA, spray-dried K3EDTA, and liquid K3EDTA anticoagulated blood samples for routine blood center or transfusion service testing

“…1,2 EDTA in blood collection tubes may induce platelet agglutination and falsely low platelet counts (EDTA-dependent pseudothrombocytopenia), [3][4][5][6][7][8][9][10][11] requiring specific validation of K 2 EDTA-and K 3 EDTAanticoagulated blood samples for various hematology analyzers. [12][13][14][15] Also, EDTA in blood collection tubes has been reported to cause agglutination of RBCs tested in blood banks, [16][17][18][19][20] to inhibit the detection of anti-A 1 when reagent RBCs were suspended in EDTA, 21 and to cause prolonged in vitro hemolysis in samples collected from a patient after a delayed hemolytic reaction. 22 Therefore, it is important that new EDTAanticoagulated blood collection tubes be validated for their suitability for routine blood bank testing.…”