Evidence of acrylamide induced oxidative stress and neurotoxicity in Drosophila melanogaster – Its amelioration with spice active enrichment: Relevance to neuropathy

Prasad, Sathya N; Muralidhara, .

doi:10.1016/j.neuro.2012.07.006

Cited by 87 publications

(47 citation statements)

References 65 publications

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“…Environmental toxins cause elevation in AchE activity and is associated with oxidative stress in neurological dysfunction, owing to decrease in acethylcholine levels in the brain [48]. Previous studies have demonstrated that rotenone and acrylamide also increased the activity of AchE in Drosophila [49,50]. In the present study, PQ exposure also caused elevation of AchE activity, and it can be as an Fig.…”

Section: Discussionsupporting

confidence: 71%

Neuroprotective Effect of Decalepis hamiltonii in Paraquat-Induced Neurotoxicity in Drosophila melanogaster: Biochemical and Behavioral Evidences

et al. 2013

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In this paper, we have demonstrated for the first time, the antioxidant and neuroprotective effects of Decalepis hamiltonii (Dh) root extract against paraquat (PQ)-induced oxidative stress and neurotoxicity in Drosophila melanogaster. Exposure of adult D. melanogaster (Oregon K) to PQ induced oxidative stress as evidenced by glutathione depletion, lipid peroxidation and enhanced activities of antioxidant enzymes such as catalase, superoxide dismutase as well as elevated levels of acetylcholine esterase. Pretreatment of flies by feeding with Dh extract (0.1, 0.5 %) for 14 days boosted the activities of antioxidant enzymes and prevented the PQ-induced oxidative stress. Dietary feeding of Dh extract prior to PQ exposure showed a lower incidence of mortality and enhanced motor activities of flies in a negative geotaxis assay; both suggesting the neuroprotective potential of Dh. Based on the results, we contemplate that the roots of Dh might prevent and ameliorate the human diseases caused by oxidative stress. The neuroprotective action of Dh can be attributed to the antioxidant constituents while the precise mechanism of its action needs further investigations.

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Section: Discussionsupporting

confidence: 71%

Neuroprotective Effect of Decalepis hamiltonii in Paraquat-Induced Neurotoxicity in Drosophila melanogaster: Biochemical and Behavioral Evidences

et al. 2013

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“…In this regard, the use of Drosophila as a PD model is rather well accepted by numerous researchers (Hirth 2010;Rand, 2010). Earlier our findings have shown the advantages and reproducibility of the ROT model and the efficacy of select medicinal plant extracts and spice active principles to attenuate oxidative stress and neurotoxicity in the Drosophila system (Hosamani and Muralidhara, 2009;Girish and Muralidhara, 2012;Prasad and Muralidhara, 2012). Accordingly, we have investigated the propensity of WS supplements to offset rotenone -induced oxidative stress, locomotor phenotype and mitochondrial dysfunctions in wild Drosophila .…”

Section: Discussionmentioning

confidence: 91%

“…Previously, we have successfully employed Drosophila as a model to understand the neuromodulatory properties of medicinal plants , spice bioactives (Prasad and Muralidhara 2012) and the Pteridophyte, Selaginella (Girish and Muralidhara 2012).…”

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confidence: 99%

Standardized extract of Withania somnifera (Ashwagandha) markedly offsets rotenone-induced locomotor deficits, oxidative impairments and neurotoxicity in Drosophila melanogaster

Manjunath

Muralidhara

2013

J Food Sci Technol

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Withania somnifera (Ashwagandha, WS) or Indian ginseng possesses multiple pharmacological properties which are mainly attributed to the active constituents, withanolides. Despite its extensive usage as a memory enhancer and a nerve tonic, few attempts have been made to ascertain its usage in the management of Parkinson's disease. In the present study, we investigated the neuroameliorative effects of WS in a rotenone (ROT) model of Drosophila melanogaster (Oregon-K). Initially, we ascertained the ability of WSenriched diet (0-0.05 %) to protect against ROT induced lethality and locomotor phenotype in adult male flies. Further, employing a co-exposure paradigm, we investigated the propensity of WS to offset ROT-induced oxidative stress, mitochondrial dysfunctions and neurotoxicity. WS conferred significant protection against ROT-induced lethality, while the survivor flies exhibited improved locomotor phenotype. Biochemical investigations revealed that ROT-induced oxidative stress was significantly diminished by WS enrichment. WS caused significant elevation in the levels of reduced GSH/ non-protein thiols. Furthermore, the altered activity levels of succinate dehydrogenase, MTT, membrane bound enzymes viz., NADH-cytochrome-c reductase and succinatecytochrome-c reductase were markedly restored to normalcy. Interestingly, ROT-induced perturbations in cholinergic function and depletion in dopamine levels were normalized by WS. Taken together these data suggests that the neuromodulatory effect of WS against ROT-induced neurotoxicity is probably mediated via suppression of oxidative stress and its potential to attenuate mitochondrial dysfunctions. Our further studies aim to understand the underlying neuroprotective mechanisms of WS and withanolides employing neuronal cell models.

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“…In recent times, the modulatory role of several nutraceuticals to attenuate endogenous redox status has been considered as an effective approach to achieve neuroprotection (Jimenez-Del-Rio et al, 2010;Dumont and Beal, 2011;Prasad and Muralidhara, 2012). Saffron extracts and its bioactive constituents have been demonstrated to possess excellent antioxidant properties (Hosseinzadeh et al, 2009;Karimi et al, 2010;Mashmoul et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Evidence of neuroprotective effects of saffron and crocin in a Drosophila model of parkinsonism

et al. 2016

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Evidence of acrylamide induced oxidative stress and neurotoxicity in Drosophila melanogaster – Its amelioration with spice active enrichment: Relevance to neuropathy

Cited by 87 publications

References 65 publications

Neuroprotective Effect of Decalepis hamiltonii in Paraquat-Induced Neurotoxicity in Drosophila melanogaster: Biochemical and Behavioral Evidences

Neuroprotective Effect of Decalepis hamiltonii in Paraquat-Induced Neurotoxicity in Drosophila melanogaster: Biochemical and Behavioral Evidences

Standardized extract of Withania somnifera (Ashwagandha) markedly offsets rotenone-induced locomotor deficits, oxidative impairments and neurotoxicity in Drosophila melanogaster

Evidence of neuroprotective effects of saffron and crocin in a Drosophila model of parkinsonism

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