Evidence of a wall friction effect in the consolidation of beds of packing materials in chromatographic columns

Guiochon, Georges; Drumm, Eric C.; Cherrak, Djamel E

doi:10.1016/s0021-9673(98)01068-1

Cited by 42 publications

(25 citation statements)

References 19 publications

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“…The load is changed at convenient time intervals such that the estimated degree of consolidation exceeds 90%. In the tests made on the packing materials which are reported here, a load increase was applied to the sample every 24 h. This was consistent with laboratory observations that the whole primary consolidation of the sample was well completed after less than 24 h. This is also consistent with observations made in chromatography that the beds are consoli-Ž dated after less than an hour Guiochon et al, 1999;Guiochon and Sarker, 1995;Sarker et al, 1996;Stanley et al, 1996;. Yew et al, 2003 .…”

Section: Methodssupporting

confidence: 86%

“…After Terzaghi 1925and Taylor 1948 Guiochon and Sarker, 1995 . This result agrees with the common observation that column beds consolidate rapidly Ž under stress Guiochon et al, 1999;Guiochon and Sarker, . 1995;Sarker et al, 1996 .…”

Section: Introductionsupporting

confidence: 92%

“…11 and a shear strength at the interface between the wall of a chromatographic column and a bed of Zorbax C18 prepared from slurries of the material in different solvents which Ž . depends on the nature of the solvent Guiochon et al, 1999 . This aspect of the problem warrants further investigation.…”

Section: Shear Strength Of the Interface Between Packing Materials Andmentioning

confidence: 99%

“…Since then, direct observations have demonstrated that there is friction between the column Ž . bed and its wall Guiochon et al, 1999 . This result validated the earlier conclusions of the review regarding the origin of the radial heterogeneity of the column bed.…”

Section: Introductionmentioning

confidence: 99%

“…It is common to observe a slow, small shrinkage of a column bed during measurements made over periods of several weeks, even though consolidation seemed to have been completed within the first few minutes of the bed compres-Ž . sion Guiochon et al, 1999 . While the first consolidation of a newly packed column bed is accompanied by an important and irreversible decrease in its length, further consolidations after unloading are nearly Ž reversible Cherrak et al, 2001;Guiochon et al, 1999;Guio-. chon and Sarker, 1995;Sarker et al, 1996;Stanley et al, 1996 and follow the law of elasticity, as expected for these particulate materials.…”

Section: Introductionmentioning

confidence: 99%

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Mechanics of column beds: I. Acquisition of the relevant parameters

2003

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The efficiency of chromatographic columns is ad®ersely affected by large-scale radial ®ariations of the packing density or ®oid ratio of the material used to prepare the bed. This heterogeneity is due to wall friction effects that take place during the preparation of the column and to seepage effects operating during the packing process and the subsequent operation of the column. The dependence of the bed's ®oid fraction on the stress applied during its consolidation was determined, as well as its permeability at ®arious stages of the consolidation process and the coefficient of friction between typical packing materials and the stainless steel wall of chromatographic columns. These results are required to de®elop and use numerical models of the ®olumetric response to axial compression of the bed and models of the coupled mechanical-seepage rheology of particulate materials.

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Section: Methodssupporting

confidence: 86%

Section: Introductionsupporting

confidence: 92%

Section: Shear Strength Of the Interface Between Packing Materials Andmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mechanics of column beds: I. Acquisition of the relevant parameters

2003

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Large‐Scale Chromatography Columns, Modeling Flow Distribution

Fang

2010

Encyclopedia of Industrial Biotechnology

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Liquid chromatography has become an indispensable tool in the pharmaceutical and biotechnology industries for preparative and large‐scale purifications to obtain high purity biopharmaceutical products. However, imperfect packing of the column bed and complex rheological behaviors of resin particles pose a great challenge for any modeling effort. A systematic study, based on rigorous physics, which attempts to understand the intrinsic complicated interrelationships between column packing, hardware design, flow distribution, and separation efficiency would be of great help to chromatography practitioners. Seeing this need, this article makes an effort to perform a systematic study of the chromatography processes from start to finish and to provide mathematical models and case studies for each stage. First, the author discusses the challenges in scaling up chromatography column packing and examines the wall effect through a dimensional analysis, then follows it by introducing a one‐dimensional model, describing column packing and scale‐up. Next, the author presents a case study on a commercial scale column with a special headplate design and investigates the impact of hardware design on flow distribution, using the technique of computational fluid dynamics (CFD). Finally, this article presents simulations on the transport of elute and computation on the height equivalent to a theoretic plate (HETP) in a modeled, large column set for no retention, ion exchange, and affinity systems. The author discovers that the hardware design exerts a great impact on the flow distribution in the columns and thus affects the column separation efficiency to a large extent.

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