Evidence of a major genetic factor conditioning freezing sensitivity in winter wheat

Abstract

The inheritance of freezing tolerance in F 2 -derived F 4 populations from all possible crosses of winter wheat cultivars ÔKestrelÕ, ÔEltanÕ, ÔTiberÕ, ÔFroidÕ and germplasm line Oregon Feed Wheat #5 (ORFW) was investigated. When frozen to a temperature equal to the LT 50 of the least freezing tolerant parent (ORFW), survival frequency distributions were skewed to greater survival in six of the 10 crosses, however, very few of the progeny from the four crosses to ORFW survived. The inheritance of this freezing sensitivity was investigated with freezing of F 2:4 populations from the crosses of ORFW to ÔEltanÕ or ÔTiberÕ to the LT 50 of the hardier parent. Very few of the F 2:4 populations survived as well as ÔEltanÕ or ÔTiberÕ, indicating a small number of strongly dominant genetic factors in ORFW that conditioned freezing sensitivity. Molecular analysis indicated these factors were not spring-type vernalization alleles.

Differential Response of Wheat Cultivars to Components of the Freezing Process in Saturated Soil

“…These clusters appear to represent groups of cultivars that employ somewhat diff erent combinations of mechanisms in their response to the various stresses imposed by the freezing process. This possibility is supported by previously published results (Skinner and Garland-Campbell, 2008). 1), the levels of freezing tolerance were very similar (Table 3), but the clear distinction of the clusters suggested that diff erent mechanisms were used in developing those similar levels of tolerance.…”

“…Among the cultivars comprising clusters 1 and 2 (Fig. 2 in Skinner and Garland-Campbell, 2008). For example, Froid and Kestrel expressed a level of freezing tolerance that was significantly greater than all other cultivars except Norstar (Table 3), yet Froid and Kestrel were grouped into diff erent clusters by their responses to the freezing process components (Fig.…”

“…For example, Froid and Kestrel expressed a level of freezing tolerance that was significantly greater than all other cultivars except Norstar (Table 3), yet Froid and Kestrel were grouped into diff erent clusters by their responses to the freezing process components (Fig. Thus, the broad variation in the Tiber by Kestrel progeny (Skinner and Garland-Campbell, 2008) was consistent with the hypothesis that cultivars distinguished by their responses to components of the freezing process ( Fig. This result suggested that high levels of freezing tolerance can be eff ected by multiple mechanisms and that the examination of the response of cultivars to the components of the freezing process can reveal groups of cultivars that use those diff erent mechanisms.…”

Phospholipase A2 Activity during Cold Acclimation of Wheat

“…Winter wheat cultivar Eltan (Peterson et al, 1991), spring wheat cultivar Louise (Kidwell et al, 2006), and germplasm line Oregon Feed Wheat #5 (described in DeNoma, 1990; Skinner and Garland‐Campbell, 2008) were used. Twenty seeds of each wheat line were planted into commercial soilless planting mix in each 3.8‐cm by 3.8‐cm by 6.3‐cm cell of a six‐cell insert in a horticultural growing tray (Model 806, Hummert, St. Louis, MO).…”

Dissecting the genetic architecture of frost tolerance in Central European winter wheat

“…Therefore, the genetic architecture of frost tolerance has been investigated for almost a century (Skinner and Garland-Campbell, 2008; Galiba et al , 2009). One prominent frost tolerance locus is Fr-1 (Sutka and Snape, 1989; McIntosh et al , 1998), which maps close to Vrn-A1 and thus has been supposed to represent a pleiotropic effect of Vrn-A1 (Dhillon et al , 2010).…”