Evidence in the Legionella pneumophila genome for exploitation of host cell functions and high genome plasticity

Cazalet, Christel; Rusniok, Christophe; Brüggemann, Holger; Zidane, Nora; Magnier, A.; Ma, Laurence; Tichit, Magalie; Jarraud, Sophie; Bouchier, Christiane; Vandenesch, François; Kunst, Frank; Etienne, Jérôme; Glaser, Philippe; Buchrieser, Carmen

doi:10.1038/ng1447

Cited by 588 publications

(774 citation statements)

References 48 publications

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“…As reported for L. pneumophila Paris (Cazalet et al, 2004), L. oakridgensis seems to exhibit a T5SS, a putative outer membrane autotransporter protein with no significant identity to any known Legionella protein (Fig. 5E).…”

Section: General Features Of the Genomesupporting

confidence: 61%

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Legionella oakridgensis ATCC 33761 genome sequence and phenotypic characterization reveals its replication capacity in amoebae

Brzuszkiewicz

Schulz

Rydzewski

et al. 2013

International Journal of Medical Microbiology

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Legionella oakridgensis is able to cause Legionnaires` disease, but is less virulent compared to L. pneumophila strains and very rarely associated with human disease. L. oakridgensis is the only species of the family legionellae which is able to grow on media without additional cysteine. In contrast to earlier publications, we found that L. oakridgensis is able to multiply in amoebae. We sequenced the genome of L. oakridgensis type strain OR-10 (ATCC 33761).The genome is smaller than the other yet sequenced Legionella genomes and has a higher G+C-content of 40.9%. L. oakridgensis lacks a flagellum and it also lacks all genes of the flagellar regulon except of the alternative sigma-28 factor FliA and the anti-sigma-28 factor

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Section: General Features Of the Genomesupporting

confidence: 61%

“…Similar plasmids have also been described for L. pneumophila Paris and Lens (Cazalet et al, 2004). In general, plasmids are described for L. micdadei, L. longbeachae, L. gormanii and L.…”

Section: General Features Of the Genomementioning

confidence: 60%

Legionella oakridgensis ATCC 33761 genome sequence and phenotypic characterization reveals its replication capacity in amoebae

Brzuszkiewicz

Schulz

Rydzewski

et al. 2013

International Journal of Medical Microbiology

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“…Experiments were done with L. pneumophila Sg1 strain Corby (Jepras et al 1985) and L. pneumophila Sg1 strain Paris (Cazalet et al 2004) and their isogenic ΔrpoN, ΔfleQ and ΔfliA mutant strains (Heuner et al 2002;Jacobi et al 2004;Albert-Weissenberger et al 2010;Brüggemann et al 2006). Further Corby strains used in this study were ΔflaA, ΔmotA and ΔfliD (Dietrich 2000;Heuner and Albert-Weissenberger 2008).…”

Section: Bacterial Strains and Amoebamentioning

confidence: 99%

“…It seems as if fliA belongs to the class III genes in strain Paris, but is not directly linked to the flagellar regulon in strain Corby (Albert-Weissenberger et al 2010;Jacobi et al 2004). Both strains are virulent L. pneumophila Sg1 isolates with only small differences within their genomes (Cazalet et al 2004;Jepras et al 1985;Glöckner et al 2008). Our intention was to clarify the regulation of fliA expression in L. pneumophila and to examine the differences between both strains.…”

Section: Introductionmentioning

confidence: 99%

FliA expression analysis and influence of the regulatory proteins RpoN, FleQ and FliA on virulence and in vivo fitness in Legionella pneumophila

et al. 2012

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“…However the SBT is rather expensive and might therefore not applicable to all strains and in all laboratories. The specific gene content of each strain sequenced so far is constituted of 7-11% of gene specific to each genome [10][11][12]. In addition, variable elements/ pathogenicity island are present in some strains [13].…”

mentioning

confidence: 99%

Variable genetic element typing: a quick method for epidemiological subtyping of Legionella pneumophila

Pannier

Heuner

Lück

2010

Eur J Clin Microbiol Infect Dis

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A total of 57 isolates of L. pneumophila were randomly selected from the German National Legionella strain collection and typed by monoclonal antibody subgrouping, seven gene locus sequence-based typing (SBT) scheme and a newly developed variable element typing (VET) system based on the presence or absence of ten variable genetic elements. These elements were detected while screening of a genomic library of strain Corby as well as taken from published data for PAI-1 (pathogenicity island) from strain Philadelphia. Specific primers were designed and used in gel based PCRs. PCR amplification of the mip gene that served as a control. The endpoint was the presence / absence of a PCR product on an ethidiumbromide strained gel. In the present study the index of discrimination was somewhat lower than that of the SBT (0.87 versus 0.97). Nevertheless, the results obtained showed as a 'proof of principle' that this simple and quick typing assay might be useful for the epidemiological characterization of Legionella pneumophila strains.

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Evidence in the Legionella pneumophila genome for exploitation of host cell functions and high genome plasticity

Cited by 588 publications

References 48 publications

Legionella oakridgensis ATCC 33761 genome sequence and phenotypic characterization reveals its replication capacity in amoebae

Legionella oakridgensis ATCC 33761 genome sequence and phenotypic characterization reveals its replication capacity in amoebae

FliA expression analysis and influence of the regulatory proteins RpoN, FleQ and FliA on virulence and in vivo fitness in Legionella pneumophila

Variable genetic element typing: a quick method for epidemiological subtyping of Legionella pneumophila

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