Evidence forPPC1,a Determinant of the Pilei-Pellis Color ofAgaricus bisporusFruitbodies

An expanded genetic linkage map of an intervarietal Agaricus bisporus var. bisporus×A. bisporus var. burnettii hybrid based on AFLP, SSR and CAPS markers sheds light on the recombination behaviour of the species

“…For example, on LG IV, the recombination cold spot bracketed by PR047 and PR089 markers symbolised the centromeric position of the chromosome. This could be confirmed by the analysis of the heterokaryotic progeny of the intervarietal hybrid as already done by Callac et al (1998). Conversely, gaps located in the distal position of linkage groups, as observed on LG X or LG VIII, may result from higher recombination rates in the telomeric regions of chromosomes or from the lack of polymorphism.…”

“…The phenotypic MAT, BSN, and PPC1 loci and the alcohol dehydrogenase (ADH) allozyme marker, previously described (Callac et al, 1998;Imbernon et al, 1996;Moquet et al, 1999), were included in the mapping data.…”

Quantitative Trait Locus Mapping of Yield-Related Components and Oligogenic Control of the Cap Color of the Button Mushroom, Agaricus bisporus

“…bisporus strains. The population used in our study consisted of 89 second-generation hybrids obtained by crossing the homokaryotic offspring (H i ) of the hybrid JB3-83 ϫ U1-7 with the homokaryon U1-2 (3,10). The parental strains JB3 (wild isolate; ATCC 200853; ARP Collection) and U1 (commercial strain; Somycel, Langeais, France), the first-generation hybrid JB3-83 ϫ U1-7 (11), and three commercial cultivars (Amycel 2100, Euromycel 25, and Euromycel 48) were used as controls in the experiments.…”

“…More than 90% of the A. bisporus mushrooms cultivated in the world have a white pileus color, in line with consumer preferences. For this important quality trait, a locus (PPC1) had been identified and related to DNA markers (3,20), but our own observations during breeding programs showed that secondary loci may be involved in fine regulation of the cap color. The recent development of a comprehensive linkage map of A. bisporus (8) makes it possible to investigate the inheritance of complex traits in the species through QTL analyses.…”

Development of AFLP markers and phylogenetic analysis in Hypsizygus marmoreus

“…According to Sinden (1990), the two traditional classes of white Agaricus bisporus cultivars, white and off-white, could be mutant types from the wild colored types during cultivation. Callac et al (1998) demonstrated that the white pileipellis trait of A. bisporus was determined by a recessive allele at a single locus (PPC1). In another study with Ceratocystis resinifera, a sap-staining fungus, a spontaneous albino mutant was detected as a point mutation in the polyketide synthase gene (PKS1) (Tanguay et al, 2006).…”