Evidence for widespread Leishmania infantum infection among wild carnivores in L. infantum periendemic northern Spain

Rı́o, Laura Del; Chitimia, Lidia; Cubas, A.; Victoriano, I.; Rúa, Pilar De la; Gerrikagoitia, Xeider; Barral, Marta; Muñoz-García, Claudia I.; Goyena, E.; García‐Martínez, Daniel; Fisa, Roser; Riera, Cristina; Murcia, Laura; Segovia, Manuel; Berriatua, E.

doi:10.1016/j.prevetmed.2013.12.001

Cited by 53 publications

(57 citation statements)

References 27 publications

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“…The potential role of wildlife species as reservoirs of the parasite should be taken into account as well. Among mustelids, L. infantum infection has been identified by PCR in liver and/or spleen tissue samples from a polecat (Mustela putorius), a European mink (Mustela lutreola) and other wild mustelids (Meles meles, Martes foina and Martes martes) in the northern Iberian Peninsula (Spain) (Del Río et al, 2014). None of the animals showed typical canine leishmaniosis lesions at post-mortem examination.…”

Section: Introductionmentioning

confidence: 99%

First report on natural infection with Leishmania infantum in a domestic ferret (Mustela putorius furo) in Spain

Giner

Basurco

Alcover

et al. 2020

Veterinary Parasitology: Regional Studies and Reports

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Section: Introductionmentioning

confidence: 99%

First report on natural infection with Leishmania infantum in a domestic ferret (Mustela putorius furo) in Spain

Giner

Basurco

Alcover

et al. 2020

Veterinary Parasitology: Regional Studies and Reports

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“…Whilst the dog is the main reservoir host of the parasite in the domestic environment, humans are considered an accidental host (Otranto et al, ; Quinnell & Courtenay, ). Moreover, other mammals, including a wide range of wildlife, have been molecularly diagnosed as L. infantum ‐positive (Del Río et al, ; Millán, Ferroglio, & Solano‐Gallego, ; Risueño et al, ). However, clinical signs are rarely reported in wildlife and most infected individuals have low parasite loads.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and phylogenetic relationships between Leishmania infantum from dogs, humans and wildlife in south‐east Spain

Ortuño

Latrofa

Iborra

et al. 2019

Zoonoses and Public Health

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Leishmania infantum causes human and canine leishmaniosis. The parasite, transmitted by phlebotomine sand flies, infects species other than dogs and people, including wildlife, although their role as reservoirs of infection remains unknown for most species. Molecular typing of parasites to investigate genetic variability and evolutionary proximity can help understand transmission cycles and designing control strategies. We investigated Leishmania DNA variability in kinetoplast (kDNA) and internal transcribed spacer 2 (ITS2) sequences in asymptomatically infected wildlife (n = 58) and symptomatically and asymptomatically infected humans (n = 38) and dogs (n = 15) from south‐east Spain, using single nucleotide polymorphisms (SNPs) and in silico restriction fragment length polymorphism (RFLP) analyses. All ITS2 sequences (n = 76) displayed a 99%–100% nucleotide identity with a L. infantum reference sequence, except one with a 98% identity to a reference Leishmania panamensis sequence, from an Ecuadorian patient. No heterogeneity was recorded in the 73 L. infantum ITS2 sequences except for one SNP in a human parasite sequence. In contrast, kDNA analysis of 44 L. infantum sequences revealed 11 SNP genotypes (nucleotide variability up to 4.3%) and four RFLP genotypes including B, F and newly described S and T genotypes. Genotype frequency was significantly greater in symptomatic compared to asymptomatic individuals. Both methods similarly grouped parasites as predominantly or exclusively found in humans, in dogs, in wildlife or in all three of them. Accordingly, the phylogenetic analysis of kDNA sequences revealed three main clusters, two as a paraphyletic human parasites clade and a third including dogs, people and wildlife parasites. Results suggest that Leishmania infantum genetics is complex even in small geographical areas and that, probably, several independent transmission cycles take place simultaneously including some connecting animals and humans. Investigating these transmission networks may be useful in understanding the transmission dynamics, infection risk and therefore in planning L. infantum control strategies.

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“…The dogis the main reservoir, although it has been demonstrated that other wild mammalscan play an important role in the maintenance and transmission of the parasite (Molina et al 2012, Del Río et al 2013, Ruiz-Fons et al 2013, Jiménez et al 2014). …”

Section: Introductionmentioning

confidence: 99%

Identification of blood meals in field captured sand flies by a PCR-RFLP approach based on cytochrome b gene

et al. 2015

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Identification of blood meals in field captured sand flies by a PCR-RFLP approach based on cytochrome b gene.Acta Tropica http://dx.doi.org/10. 1016/j.actatropica.2015.08.020 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Therefore, thisapproachis anefficient and reliable alternative method to be applied in entomological surveys.

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Evidence for widespread Leishmania infantum infection among wild carnivores in L. infantum periendemic northern Spain

Cited by 53 publications

References 27 publications

First report on natural infection with Leishmania infantum in a domestic ferret (Mustela putorius furo) in Spain

First report on natural infection with Leishmania infantum in a domestic ferret (Mustela putorius furo) in Spain

Genetic diversity and phylogenetic relationships between Leishmania infantum from dogs, humans and wildlife in south‐east Spain

Identification of blood meals in field captured sand flies by a PCR-RFLP approach based on cytochrome b gene

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