Evidence for two leukotriene receptor types in the guinea-pig isolated ileum

Gardiner, P.J.; Abram, Trevor S.; Cuthbert, Nigel J.

doi:10.1016/0014-2999(90)90288-h

Cited by 41 publications

(14 citation statements)

References 15 publications

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“…In contrast, the expected affinity of LTD 4 for CysLT 2 was approximately 50-fold lower than that of LTC 4 (10) in radioligand binding studies, while LTC 4 and LTD 4 induced calcium mobilization with equipotency (10 -12). Surprisingly, human CysLT 2 was able be detected at low levels in the lung and trachea, which were generally thought the existence of CysLT 2 in other species (7,(13)(14)(15). Interestingly, in the human heart, strong expression of CysLT 2 was observed but little or no CysLT 1 mRNA was detected (8,9).…”

mentioning

confidence: 85%

Functional Characterization of Cysteinyl Leukotriene CysLT2 Receptor on Human Coronary Artery Smooth Muscle Cells

Kamohara¹,

Takasaki²,

Matsumoto³

et al. 2001

Biochemical and Biophysical Research Communications

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mentioning

confidence: 85%

Functional Characterization of Cysteinyl Leukotriene CysLT2 Receptor on Human Coronary Artery Smooth Muscle Cells

Kamohara¹,

Takasaki²,

Matsumoto³

et al. 2001

Biochemical and Biophysical Research Communications

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“…The biological effects of LTE 4 have been studied less, perhaps because this leukotriene was found to be an incomplete and less potent agonist than LTC 4 and LTD 4 in some smooth muscle preparations such the guinea pig ileum [131][132][133]. However, LTE 4 has been documented to possess a bronchoconstrictor activity in vitro and in vivo which is very similar to that of LTC 4 and LTD 4 [134,135].…”

Section: Biological Effects Of the Cysteinyl-leukotrienesmentioning

confidence: 99%

Asthma and leukotrienes: antileukotrienes as novel anti‐asthmatic drugs

Claesson¹,

1999

Journal of Internal Medicine

101

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“…4C). Previous reports showed functional antagonism of Bay-u9773 to the contraction activities of CysLT2 in organ bath assay of guinea pig (18,19). We showed that Bay-u9773 behaved as an antagonist and a partial agonist for gpCysLT2 in intracellular calcium response as well as other species (23) and that Bay-u9773 induced cellular proliferation by itself and with the following stimulation of LTC4.…”

Section: Intracellular Calcium Elevation Through Gpcyslt2mentioning

confidence: 48%

“…Based on functional smooth muscle studies, it was reported that gpCysLT2 was expressed in the ileum (18) and trachea (19). We investigated the tissue distribution of guinea pig CysLT2 gene by quantitative real time PCR (Fig.…”

Section: Tissue Distribution Of Gpcyslt2 Expressionmentioning

confidence: 99%

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Guinea pig cysteinyl leukotriene receptor 2 (gpCysLT2) mediates cell proliferation and intracellular calcium mobilization by LTC4 and LTD4

Ito¹,

Hirano²,

Umemoto³

et al. 2008

BMB Reports

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We cloned and pharmacologically characterized the guinea pig cysteinyl leukotriene (CysLT) 2 receptor (gpCysLT2). gpCysLT2 consists of 317 amino acids with 75.3%, 75.2%, 73.3% identity to those of humans, mice and rats, respectively. The gpCysLT2 gene is highly expressed in the lung, moderately in eosinophils, skin, spleen, stomach, colon, and modestly in the small intestine. CysLTs accelerated the proliferation of gpCysLT2-expressing HEK293. Leukotriene C4 (LTC4) and Leukotriene D4 (LTD4) enhanced the cell proliferation higher than Bay-u9773, a CysLT2 selective partial agonist and a nonselective antagonist for CysLT receptors. Bay-u9773 did not antagonize the cell proliferation by LTC4 and LTD4. Despite the equipotency of the mitogenic effect among these chemicals, calcium mobilization (CM) levels were variable (LTC4＞ LTD4＞＞ Bay-u9773), and Bay-u9773 antagonized the CM by LTC4. Moreover, the Gi/o inhibitor pertussis toxin perfectly inhibited agonist-induced cell proliferation. These results reveal that cell proliferation via CysLT2 signaling was mediated by Gi/o signaling but independent of calcium mobilization. [BMB reports 2008; 41(2): 139-145]

show abstract

Evidence for two leukotriene receptor types in the guinea-pig isolated ileum

Cited by 41 publications

References 15 publications

Functional Characterization of Cysteinyl Leukotriene CysLT2 Receptor on Human Coronary Artery Smooth Muscle Cells

Functional Characterization of Cysteinyl Leukotriene CysLT2 Receptor on Human Coronary Artery Smooth Muscle Cells

Asthma and leukotrienes: antileukotrienes as novel anti‐asthmatic drugs

Guinea pig cysteinyl leukotriene receptor 2 (gpCysLT2) mediates cell proliferation and intracellular calcium mobilization by LTC4 and LTD4

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