Evidence for three separate genes encoding the proteins of the mycobacterial antigen 85 complex

Wiker, Harald G.; Sletten, K; Nagai, Shoichiro; Harboe, Morten

doi:10.1128/iai.58.1.272-274.1990

Cited by 69 publications

(49 citation statements)

References 14 publications

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“…The 85 complex proteins, which were also secreted from the mycobacteria, consisted of at least four or five proteins [26]. The homologies of these proteins in BCG were from 41% to 78% [27][28][29][30][31][32][33]. Similarly, the genes encoding MPB70 and homologues may constitute a gene family.…”

Section: Discussionmentioning

confidence: 99%

Cloning and Sequencing of an MPB70 Homologue Corresponding to MPB83 from Mycobacterium bovis BCG

Matsuo

Ohara

et al. 1996

Scand J Immunol

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MPB70 is secreted in high concentrations by Mycobacterium bovis BCG substrain Tokyo (BCG Tokyo), but little by substrains Pasteur (BCG Pasteur) and M. tuberculosis. The gene encoding a MPB70 homologue secreted by BCG Tokyo was found at the upstream region of the gene encoding MPB70, with approximately 2.3 kilobase pairs (kbp) spacing: the same gene was also found in BCG Pasteur. This gene was cloned and sequenced from BCG Tokyo. The DNA sequence which contained a 663 base pair (bp) open reading frame beginning at position 1 and ending with a TAA codon at position 661 was found. Its theoretical molecular mass was calculated to be 22.068 kDa. This gene was highly homologous to the coding region of mpb70 and the deduced amino acid sequence was very similar to MPB83 reported by Harboe et al. It was speculated that the gene the authors characterized probably corresponded to the mpb83 gene.

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Section: Discussionmentioning

confidence: 99%

Cloning and Sequencing of an MPB70 Homologue Corresponding to MPB83 from Mycobacterium bovis BCG

Matsuo

Ohara

et al. 1996

Scand J Immunol

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“…Experiments are in progress concerning similar comparison of MPB70 and MPB80 to see whether these three proteins constitute a second family of secreted mycobacterial proteins. The first family of secreted proteins [13,27] includes the antigen 85 complex, its three components 85A, 85B, and 85C being coded by three homologous genes [28,29] being localized at different sites in the genome in M.leprae [30] as well as M.tuberculosis and BCG [31]. The fourth member of this family, MPB51 [13,27] is coded by a gene localized closely to the 85 A gene in the same opcron, in M.leprae [30] as well as in BCG [32].…”

Section: Discussionmentioning

confidence: 99%

Homology Between the MPB70 and MPB83 Proteins of Mycobacterium bovis BCG

Harboe

Nagai

et al. 1995

Scand J Immunol

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Isolation of MPB83 from Mycobacterium bovis BCG Tokyo culture fluid is described. MPB70 and MPB83 have similar molecular mass as judged by SDS-PAGE but differ in isoelectric points. Peptides isolated after CNBr cleavage of MPB83 revealed extensive homology as well as distinct differences from corresponding parts of the amino acid sequence deduced from the mpb70 gene cloned by Terasaka et al. Antibodies produced by immunization with MPB70 and MPB83 had distinctly different fine specificity revealing cross-reactivity between the proteins. These findings indicate that two distinct, homologous genes code for these proteins. Sensitization with live BCG Tokyo also induced T cell responses to MPB83 with development of delayed type hypersensitivity in guinea pigs.

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“…Interestingly, very recent results from Belisle and colleages indicate that AG85B holds mycolyl-transferase activity indicating that outer cell wall synthesis may be the primary function of this molecule [165]. The three components are encoded by three separate genes [166,167]. Signal sequences have been identified.…”

Section: Culture Filtrate Proteinsmentioning

confidence: 99%

Host Responses and Antigens Involved in Protective Immunity to Mycobacterium tuberculosis

1997

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Andersen P. Host Responses and Antigens Involved in Protective Immunity to Mycobacterium tuberculosis. Scand J Immunol 1997;45:115-131 Tuberculosis (TB) is the largest single infectious cause of human mortality. The incidence of TB has remained high in most of the developing world and the disease has recently re-emerged as a public health problem in industrialized countries. The development of a new improved TB vaccine is a highly prioritized international research area, which has been further stimulated by the appearance of multi-drug resistant strains of Mycobacterium tuberculosis. The present status of the attempts to characterize the protective immune response to TB will be reviewed with special emphasis on recent progress in the identification and characterization of target molecules recognized by protective cells. This paper will focus on proteins released from live bacteria and discuss their role in the host-pathogen interaction and the ongoing attempts to use these molecules in TB subunit vaccines.

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Evidence for three separate genes encoding the proteins of the mycobacterial antigen 85 complex

Cited by 69 publications

References 14 publications

Cloning and Sequencing of an MPB70 Homologue Corresponding to MPB83 from Mycobacterium bovis BCG

Cloning and Sequencing of an MPB70 Homologue Corresponding to MPB83 from Mycobacterium bovis BCG

Homology Between the MPB70 and MPB83 Proteins of Mycobacterium bovis BCG

Host Responses and Antigens Involved in Protective Immunity to Mycobacterium tuberculosis

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