Evidence for the production of hydroxyl radicals from the adriamycin semiquinone and H<sub>2</sub>O<sub>2</sub>

Winterbourn, Christine C.

doi:10.1016/0014-5793(81)81220-3

Cited by 80 publications

(21 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With the use of electron paramagnetic resonance (EPR) spectroscopy to directly detect radicals in vivo (18), we observed that hyperthermia progressively increased portal venous content of the following: semiquinone radical, a biomarker of mitochondrial reductive stress (52); ceruloplasmin, an acute phase antioxidant protein that acts to reduce metal-catalyzed oxidant production (29); and penta-coordinate iron(II) nitrosyl-heme (heme-⅐NO) (27). The respective characteristics of these radicals suggest that heat stress stimulates ⅐NO and reactive oxygen species (ROS) production within splanchnic viscera leading to transition metal activation and cellular oxidative stress.…”

mentioning

confidence: 99%

Mechanisms of circulatory and intestinal barrier dysfunction during whole body hyperthermia

Hall¹,

Buettner

Oberley

et al. 2001

American Journal of Physiology-Heart and Circulatory Physiology

386

338

View full text Add to dashboard Cite

This work tested the hypotheses that splanchnic oxidant generation is important in determining heat tolerance and that inappropriate.NO production may be involved in circulatory dysfunction with heat stroke. We monitored colonic temperature (T(c)), heart rate, mean arterial pressure, and splanchnic blood flow (SBF) in anesthetized rats exposed to 40 degrees C ambient temperature. Heating rate, heating time, and thermal load determined heat tolerance. Portal blood was regularly collected for determination of radical and endotoxin content. Elevating T(c) from 37 to 41.5 degrees C reduced SBF by 40% and stimulated production of the radicals ceruloplasmin, semiquinone, and penta-coordinate iron(II) nitrosyl-heme (heme-.NO). Portal endotoxin concentration rose from 28 to 59 pg/ml (P < 0.05). Compared with heat stress alone, heat plus treatment with the nitric oxide synthase (NOS) antagonist N(omega)-nitro-L-arginine methyl ester (L-NAME) dose dependently depressed heme-.NO production and increased ceruloplasmin and semiquinone levels. L-NAME also significantly reduced lowered SBF, increased portal endotoxin concentration, and reduced heat tolerance (P < 0.05). The NOS II and diamine oxidase antagonist aminoguanidine, the superoxide anion scavenger superoxide dismutase, and the xanthine oxidase antagonist allopurinol slowed the rates of heme-.NO production, decreased ceruloplasmin and semiquinone levels, and preserved SBF. However, only aminoguanidine and allopurinol improved heat tolerance, and only allpourinol eliminated the rise in portal endotoxin content. We conclude that hyperthermia stimulates xanthine oxidase production of reactive oxygen species that activate metals and limit heat tolerance by promoting circulatory and intestinal barrier dysfunction. In addition, intact NOS activity is required for normal stress tolerance, whereas overproduction of.NO may contribute to the nonprogrammed splanchnic dilation that precedes vascular collapse with heat stroke.

show abstract

mentioning

confidence: 99%

Mechanisms of circulatory and intestinal barrier dysfunction during whole body hyperthermia

Hall¹,

Buettner

Oberley

et al. 2001

American Journal of Physiology-Heart and Circulatory Physiology

386

338

View full text Add to dashboard Cite

show abstract

“…Subsequent autoxidation causes the catalytic production of oxygencentred radicals [2]. Recently adriamycin semiquinone, generated by the reduction of adriamycin by superoxide radicals formed from the reaction of xanthine oxidase with xanthine, in combination with hydrogen peroxide has been shown to generate hydroxyl radicals [4]. However, despite suggestive evidence using isolated microsomes and enzyme systems, the definite generation of inactive oxygen species in intact cells by adriamycin has not yet been demonstrated.…”

Section: Introduction 2 Materials and Methodsmentioning

confidence: 99%

The production of hydroxyl radicals by adriamycin in red blood cells

Bannister

Thornalley

1983

FEBS Letters

View full text Add to dashboard Cite

Spin trapping of the free radicals formed from the interaction between adriamycin and red blood cells resulted in the formation of a hydroxyl spin adduct. The formation of hydroxyl radicah was found to be inhibited by mannitol. Hemoglobin was found not to be obligatory for the formation of hydroxyl radicals which probably result from the reduction of hydrogen peroxide by adriamycin semiquinone. AdriamycinRed blood cell EPR Spin trapping Hydroxyl radical

show abstract

“…Winterbourn et al (113,114) also found that hydroxyl radicals can also be formed from the reaction between adriamycin semiquinone or paraquat radical and H 2 0 2 • Oxygen was not required but a ferric catalyst may be required.…”

Section: Dxfe 2 + + H 2 0 2 ) DX -Fe 3 + + Oh-+ Oh·mentioning

confidence: 95%

Mechanisms of Membrane Lipid Peroxidation

McGirr

O’Brien

1985

Recent Advances in Biological Membrane Studies

View full text Add to dashboard Cite

Evidence for the production of hydroxyl radicals from the adriamycin semiquinone and H₂O₂

Cited by 80 publications

References 20 publications

Mechanisms of circulatory and intestinal barrier dysfunction during whole body hyperthermia

Mechanisms of circulatory and intestinal barrier dysfunction during whole body hyperthermia

The production of hydroxyl radicals by adriamycin in red blood cells

Mechanisms of Membrane Lipid Peroxidation

Contact Info

Product

Resources

About

Evidence for the production of hydroxyl radicals from the adriamycin semiquinone and H2O2

Cited by 80 publications

References 20 publications

Mechanisms of circulatory and intestinal barrier dysfunction during whole body hyperthermia

Mechanisms of circulatory and intestinal barrier dysfunction during whole body hyperthermia

The production of hydroxyl radicals by adriamycin in red blood cells

Mechanisms of Membrane Lipid Peroxidation

Contact Info

Product

Resources

About

Evidence for the production of hydroxyl radicals from the adriamycin semiquinone and H₂O₂